A BIDISPERSE MODEL TO STUDY THE HYDROLYSIS OF MALTOSE USING GLUCOAMYLASE IMMOBILIZED IN SILICA AND WRAPPED IN PECTIN GEL

Detalhes bibliográficos
Autor(a) principal: Gonçalves,L.R.B.
Data de Publicação: 1997
Outros Autores: Giordano,R.L.C., Giordano,R.C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Chemical Engineering
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321997000400006
Resumo: In this work, a bidisperse model is built to represent the hydrolysis of maltose using immobilized glucoamylase. The experimental set is a mixed-batch reactor, maintained at 30ºC, with pectin gel spherical particles that contain enzyme immobilized in macroporous silica. The possibility of substrate adsorption on the pectin gel is also studied because this phenomenon may result in smaller values of diffusivity. Equilibrium assays are then performed for different substrates (maltose, lactose and glucose) at different temperatures and pHs. These assays show that adsorption on the pectin gel is not important for the three dextrins analysed. The bidisperse model presents a good fit with the experimental data, when using previously-estimated kinetic and mass transfer parameters (Gonçalves et al., 1997). This result shows that the methodology used (wrapping the silica in pectin gel) is appropriate for experimental studies with silica, since it allows a higher degree of agitation without causing shearing
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spelling A BIDISPERSE MODEL TO STUDY THE HYDROLYSIS OF MALTOSE USING GLUCOAMYLASE IMMOBILIZED IN SILICA AND WRAPPED IN PECTIN GELMathematical modelsmaltose hydrolysisprocess simulationIn this work, a bidisperse model is built to represent the hydrolysis of maltose using immobilized glucoamylase. The experimental set is a mixed-batch reactor, maintained at 30ºC, with pectin gel spherical particles that contain enzyme immobilized in macroporous silica. The possibility of substrate adsorption on the pectin gel is also studied because this phenomenon may result in smaller values of diffusivity. Equilibrium assays are then performed for different substrates (maltose, lactose and glucose) at different temperatures and pHs. These assays show that adsorption on the pectin gel is not important for the three dextrins analysed. The bidisperse model presents a good fit with the experimental data, when using previously-estimated kinetic and mass transfer parameters (Gonçalves et al., 1997). This result shows that the methodology used (wrapping the silica in pectin gel) is appropriate for experimental studies with silica, since it allows a higher degree of agitation without causing shearingBrazilian Society of Chemical Engineering1997-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321997000400006Brazilian Journal of Chemical Engineering v.14 n.4 1997reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66321997000400006info:eu-repo/semantics/openAccessGonçalves,L.R.B.Giordano,R.L.C.Giordano,R.C.eng1998-10-06T00:00:00Zoai:scielo:S0104-66321997000400006Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:1998-10-06T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false
dc.title.none.fl_str_mv A BIDISPERSE MODEL TO STUDY THE HYDROLYSIS OF MALTOSE USING GLUCOAMYLASE IMMOBILIZED IN SILICA AND WRAPPED IN PECTIN GEL
title A BIDISPERSE MODEL TO STUDY THE HYDROLYSIS OF MALTOSE USING GLUCOAMYLASE IMMOBILIZED IN SILICA AND WRAPPED IN PECTIN GEL
spellingShingle A BIDISPERSE MODEL TO STUDY THE HYDROLYSIS OF MALTOSE USING GLUCOAMYLASE IMMOBILIZED IN SILICA AND WRAPPED IN PECTIN GEL
Gonçalves,L.R.B.
Mathematical models
maltose hydrolysis
process simulation
title_short A BIDISPERSE MODEL TO STUDY THE HYDROLYSIS OF MALTOSE USING GLUCOAMYLASE IMMOBILIZED IN SILICA AND WRAPPED IN PECTIN GEL
title_full A BIDISPERSE MODEL TO STUDY THE HYDROLYSIS OF MALTOSE USING GLUCOAMYLASE IMMOBILIZED IN SILICA AND WRAPPED IN PECTIN GEL
title_fullStr A BIDISPERSE MODEL TO STUDY THE HYDROLYSIS OF MALTOSE USING GLUCOAMYLASE IMMOBILIZED IN SILICA AND WRAPPED IN PECTIN GEL
title_full_unstemmed A BIDISPERSE MODEL TO STUDY THE HYDROLYSIS OF MALTOSE USING GLUCOAMYLASE IMMOBILIZED IN SILICA AND WRAPPED IN PECTIN GEL
title_sort A BIDISPERSE MODEL TO STUDY THE HYDROLYSIS OF MALTOSE USING GLUCOAMYLASE IMMOBILIZED IN SILICA AND WRAPPED IN PECTIN GEL
author Gonçalves,L.R.B.
author_facet Gonçalves,L.R.B.
Giordano,R.L.C.
Giordano,R.C.
author_role author
author2 Giordano,R.L.C.
Giordano,R.C.
author2_role author
author
dc.contributor.author.fl_str_mv Gonçalves,L.R.B.
Giordano,R.L.C.
Giordano,R.C.
dc.subject.por.fl_str_mv Mathematical models
maltose hydrolysis
process simulation
topic Mathematical models
maltose hydrolysis
process simulation
description In this work, a bidisperse model is built to represent the hydrolysis of maltose using immobilized glucoamylase. The experimental set is a mixed-batch reactor, maintained at 30ºC, with pectin gel spherical particles that contain enzyme immobilized in macroporous silica. The possibility of substrate adsorption on the pectin gel is also studied because this phenomenon may result in smaller values of diffusivity. Equilibrium assays are then performed for different substrates (maltose, lactose and glucose) at different temperatures and pHs. These assays show that adsorption on the pectin gel is not important for the three dextrins analysed. The bidisperse model presents a good fit with the experimental data, when using previously-estimated kinetic and mass transfer parameters (Gonçalves et al., 1997). This result shows that the methodology used (wrapping the silica in pectin gel) is appropriate for experimental studies with silica, since it allows a higher degree of agitation without causing shearing
publishDate 1997
dc.date.none.fl_str_mv 1997-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321997000400006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66321997000400006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0104-66321997000400006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
dc.source.none.fl_str_mv Brazilian Journal of Chemical Engineering v.14 n.4 1997
reponame:Brazilian Journal of Chemical Engineering
instname:Associação Brasileira de Engenharia Química (ABEQ)
instacron:ABEQ
instname_str Associação Brasileira de Engenharia Química (ABEQ)
instacron_str ABEQ
institution ABEQ
reponame_str Brazilian Journal of Chemical Engineering
collection Brazilian Journal of Chemical Engineering
repository.name.fl_str_mv Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)
repository.mail.fl_str_mv rgiudici@usp.br||rgiudici@usp.br
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