Observance of polymorphic behaviour during dissolution of insulin and lysozyme

Detalhes bibliográficos
Autor(a) principal: Bernardo,A.
Data de Publicação: 2005
Outros Autores: Calmanovici,C. E., Miranda,E. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Chemical Engineering
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322005000300002
Resumo: Although protein crystallization is a unit operation with potentially high separation factors, it has not been widely used in industry. Protein crystallization studies and practices have hitherto been largely limited to crystallography protocols. Knowledge of the behaviour of protein in solution would help to overcome empiric limitations in protein crystallisation. Thus, dissolution of porcine insulin and hen egg white lysozyme was studied and an unusual variation in solute concentration, with a concentration peak for short dissolution times, was verified. Polymorphic behaviour of protein in solution was observed, which altered physical properties such as solubility.
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spelling Observance of polymorphic behaviour during dissolution of insulin and lysozymeInsulinLysozymeDissolutionPseudopolymorphismSolvatomorphismSolubilityAlthough protein crystallization is a unit operation with potentially high separation factors, it has not been widely used in industry. Protein crystallization studies and practices have hitherto been largely limited to crystallography protocols. Knowledge of the behaviour of protein in solution would help to overcome empiric limitations in protein crystallisation. Thus, dissolution of porcine insulin and hen egg white lysozyme was studied and an unusual variation in solute concentration, with a concentration peak for short dissolution times, was verified. Polymorphic behaviour of protein in solution was observed, which altered physical properties such as solubility.Brazilian Society of Chemical Engineering2005-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322005000300002Brazilian Journal of Chemical Engineering v.22 n.3 2005reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322005000300002info:eu-repo/semantics/openAccessBernardo,A.Calmanovici,C. E.Miranda,E. A.eng2005-09-26T00:00:00Zoai:scielo:S0104-66322005000300002Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2005-09-26T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false
dc.title.none.fl_str_mv Observance of polymorphic behaviour during dissolution of insulin and lysozyme
title Observance of polymorphic behaviour during dissolution of insulin and lysozyme
spellingShingle Observance of polymorphic behaviour during dissolution of insulin and lysozyme
Bernardo,A.
Insulin
Lysozyme
Dissolution
Pseudopolymorphism
Solvatomorphism
Solubility
title_short Observance of polymorphic behaviour during dissolution of insulin and lysozyme
title_full Observance of polymorphic behaviour during dissolution of insulin and lysozyme
title_fullStr Observance of polymorphic behaviour during dissolution of insulin and lysozyme
title_full_unstemmed Observance of polymorphic behaviour during dissolution of insulin and lysozyme
title_sort Observance of polymorphic behaviour during dissolution of insulin and lysozyme
author Bernardo,A.
author_facet Bernardo,A.
Calmanovici,C. E.
Miranda,E. A.
author_role author
author2 Calmanovici,C. E.
Miranda,E. A.
author2_role author
author
dc.contributor.author.fl_str_mv Bernardo,A.
Calmanovici,C. E.
Miranda,E. A.
dc.subject.por.fl_str_mv Insulin
Lysozyme
Dissolution
Pseudopolymorphism
Solvatomorphism
Solubility
topic Insulin
Lysozyme
Dissolution
Pseudopolymorphism
Solvatomorphism
Solubility
description Although protein crystallization is a unit operation with potentially high separation factors, it has not been widely used in industry. Protein crystallization studies and practices have hitherto been largely limited to crystallography protocols. Knowledge of the behaviour of protein in solution would help to overcome empiric limitations in protein crystallisation. Thus, dissolution of porcine insulin and hen egg white lysozyme was studied and an unusual variation in solute concentration, with a concentration peak for short dissolution times, was verified. Polymorphic behaviour of protein in solution was observed, which altered physical properties such as solubility.
publishDate 2005
dc.date.none.fl_str_mv 2005-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322005000300002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322005000300002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0104-66322005000300002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
dc.source.none.fl_str_mv Brazilian Journal of Chemical Engineering v.22 n.3 2005
reponame:Brazilian Journal of Chemical Engineering
instname:Associação Brasileira de Engenharia Química (ABEQ)
instacron:ABEQ
instname_str Associação Brasileira de Engenharia Química (ABEQ)
instacron_str ABEQ
institution ABEQ
reponame_str Brazilian Journal of Chemical Engineering
collection Brazilian Journal of Chemical Engineering
repository.name.fl_str_mv Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)
repository.mail.fl_str_mv rgiudici@usp.br||rgiudici@usp.br
_version_ 1754213171897303040

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