A pseudobiospecific hollow fiber cartridge for in vitro adsorption of autoantibodies from pathological serum

Detalhes bibliográficos
Autor(a) principal: Ventura,R.C.A.
Data de Publicação: 2000
Outros Autores: Zollner,R.L., Legallais,C., Vijayalakshmi,M.A., Bueno,S.M.A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Chemical Engineering
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322000000400052
Resumo: The affinity filtration technique using histidine as a pseudobiospecific ligand immobilized on poly(ethylene vinyl alcohol) hollow fiber membranes (His-PEVA) was used to remove autoantibodies from serum of patients with autoimmune disease. The effects of buffering solution conditions on the efficiency of autoantibodies removal was studied. The removal of anti-dsDNA, anti-SS-A/Ro, anti-Sm, anti-Sm/RNP and anti-cardiolipin autoantibodies present in the serum was investigated, comparing the efficiency between Hepes and Tris-HCl buffers. The results showed the potential of the membrane to remove all the autoantibodies studied. Anti SS-A/Ro was removed more efficiently in Tris-HCl buffer system rather than with the Hepes buffer.
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spelling A pseudobiospecific hollow fiber cartridge for in vitro adsorption of autoantibodies from pathological serumautoantibodiesauto-immune diseasesextracorporeal circulationhistidineaffinity membranesThe affinity filtration technique using histidine as a pseudobiospecific ligand immobilized on poly(ethylene vinyl alcohol) hollow fiber membranes (His-PEVA) was used to remove autoantibodies from serum of patients with autoimmune disease. The effects of buffering solution conditions on the efficiency of autoantibodies removal was studied. The removal of anti-dsDNA, anti-SS-A/Ro, anti-Sm, anti-Sm/RNP and anti-cardiolipin autoantibodies present in the serum was investigated, comparing the efficiency between Hepes and Tris-HCl buffers. The results showed the potential of the membrane to remove all the autoantibodies studied. Anti SS-A/Ro was removed more efficiently in Tris-HCl buffer system rather than with the Hepes buffer.Brazilian Society of Chemical Engineering2000-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322000000400052Brazilian Journal of Chemical Engineering v.17 n.4-7 2000reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322000000400052info:eu-repo/semantics/openAccessVentura,R.C.A.Zollner,R.L.Legallais,C.Vijayalakshmi,M.A.Bueno,S.M.A.eng2001-03-16T00:00:00Zoai:scielo:S0104-66322000000400052Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2001-03-16T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false
dc.title.none.fl_str_mv A pseudobiospecific hollow fiber cartridge for in vitro adsorption of autoantibodies from pathological serum
title A pseudobiospecific hollow fiber cartridge for in vitro adsorption of autoantibodies from pathological serum
spellingShingle A pseudobiospecific hollow fiber cartridge for in vitro adsorption of autoantibodies from pathological serum
Ventura,R.C.A.
autoantibodies
auto-immune diseases
extracorporeal circulation
histidine
affinity membranes
title_short A pseudobiospecific hollow fiber cartridge for in vitro adsorption of autoantibodies from pathological serum
title_full A pseudobiospecific hollow fiber cartridge for in vitro adsorption of autoantibodies from pathological serum
title_fullStr A pseudobiospecific hollow fiber cartridge for in vitro adsorption of autoantibodies from pathological serum
title_full_unstemmed A pseudobiospecific hollow fiber cartridge for in vitro adsorption of autoantibodies from pathological serum
title_sort A pseudobiospecific hollow fiber cartridge for in vitro adsorption of autoantibodies from pathological serum
author Ventura,R.C.A.
author_facet Ventura,R.C.A.
Zollner,R.L.
Legallais,C.
Vijayalakshmi,M.A.
Bueno,S.M.A.
author_role author
author2 Zollner,R.L.
Legallais,C.
Vijayalakshmi,M.A.
Bueno,S.M.A.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ventura,R.C.A.
Zollner,R.L.
Legallais,C.
Vijayalakshmi,M.A.
Bueno,S.M.A.
dc.subject.por.fl_str_mv autoantibodies
auto-immune diseases
extracorporeal circulation
histidine
affinity membranes
topic autoantibodies
auto-immune diseases
extracorporeal circulation
histidine
affinity membranes
description The affinity filtration technique using histidine as a pseudobiospecific ligand immobilized on poly(ethylene vinyl alcohol) hollow fiber membranes (His-PEVA) was used to remove autoantibodies from serum of patients with autoimmune disease. The effects of buffering solution conditions on the efficiency of autoantibodies removal was studied. The removal of anti-dsDNA, anti-SS-A/Ro, anti-Sm, anti-Sm/RNP and anti-cardiolipin autoantibodies present in the serum was investigated, comparing the efficiency between Hepes and Tris-HCl buffers. The results showed the potential of the membrane to remove all the autoantibodies studied. Anti SS-A/Ro was removed more efficiently in Tris-HCl buffer system rather than with the Hepes buffer.
publishDate 2000
dc.date.none.fl_str_mv 2000-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322000000400052
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322000000400052
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0104-66322000000400052
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
dc.source.none.fl_str_mv Brazilian Journal of Chemical Engineering v.17 n.4-7 2000
reponame:Brazilian Journal of Chemical Engineering
instname:Associação Brasileira de Engenharia Química (ABEQ)
instacron:ABEQ
instname_str Associação Brasileira de Engenharia Química (ABEQ)
instacron_str ABEQ
institution ABEQ
reponame_str Brazilian Journal of Chemical Engineering
collection Brazilian Journal of Chemical Engineering
repository.name.fl_str_mv Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)
repository.mail.fl_str_mv rgiudici@usp.br||rgiudici@usp.br
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