Molecular characterization of potato cultivars using SSR markers

Detalhes bibliográficos
Autor(a) principal: Favoretto,Patrícia
Data de Publicação: 2011
Outros Autores: Veasey,Elizabeth Ann, Melo,Paulo César Tavares de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Horticultura Brasileira
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000400017
Resumo: The potato crop has a very narrow genetic base, so the use of molecular markers is a very important tool in the characterization of germplasm banks and evaluation of genetic divergence. The objective of this study was to identify, using microsatellite or simple sequence repeat (SSR) markers, 38 accessions of potato from two collections of commercial cultivars. For the molecular characterization 10 loci were used, generating a total of 46 alleles, which were analyzed as binary data. A cluster analysis was performed with the Jaccard´s similarity coefficient and the UPGMA method, using the software NTSYSpc. On average, the number of alleles per locus was 4.6, ranging from two alleles for primers STM1049, STM 1053 and STM 1104 to 12 alleles per locus for primer STM0019a. Of the 46 alleles, only five were monomorphic, therefore presenting 89.1% polymorphism. The polymorphism information content (PIC) varied from 0.13 to 0.86, with an average of 0.54. The Jaccard´s coefficient varied from 0.41 to 0.93, showing high genetic variability among accessions. Two possible duplicates [Atlantic (Canada) and Atlantic (Chile), and Colorado and Ágata (EPAMIG) (duplicates with these SSRs, which did not separate them)] were identified. High similarity was also shown by cultivars Chipie and Melodie (EPAMIG), Voyager and Gourmandine (EPAMIG), Eole and Caesar (EPAMIG), and Cupido and Santé (Pirassu). The most genetically divergent accessions (Lady Rosetta and HPC-7B) were also identified.
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spelling Molecular characterization of potato cultivars using SSR markersSolanum tuberosumgermplasm banksgenetic diversitymicrosatellitesThe potato crop has a very narrow genetic base, so the use of molecular markers is a very important tool in the characterization of germplasm banks and evaluation of genetic divergence. The objective of this study was to identify, using microsatellite or simple sequence repeat (SSR) markers, 38 accessions of potato from two collections of commercial cultivars. For the molecular characterization 10 loci were used, generating a total of 46 alleles, which were analyzed as binary data. A cluster analysis was performed with the Jaccard´s similarity coefficient and the UPGMA method, using the software NTSYSpc. On average, the number of alleles per locus was 4.6, ranging from two alleles for primers STM1049, STM 1053 and STM 1104 to 12 alleles per locus for primer STM0019a. Of the 46 alleles, only five were monomorphic, therefore presenting 89.1% polymorphism. The polymorphism information content (PIC) varied from 0.13 to 0.86, with an average of 0.54. The Jaccard´s coefficient varied from 0.41 to 0.93, showing high genetic variability among accessions. Two possible duplicates [Atlantic (Canada) and Atlantic (Chile), and Colorado and Ágata (EPAMIG) (duplicates with these SSRs, which did not separate them)] were identified. High similarity was also shown by cultivars Chipie and Melodie (EPAMIG), Voyager and Gourmandine (EPAMIG), Eole and Caesar (EPAMIG), and Cupido and Santé (Pirassu). The most genetically divergent accessions (Lady Rosetta and HPC-7B) were also identified.Associação Brasileira de Horticultura2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000400017Horticultura Brasileira v.29 n.4 2011reponame:Horticultura Brasileirainstname:Associação Brasileira de Horticultura (ABH)instacron:ABH10.1590/S0102-05362011000400017info:eu-repo/semantics/openAccessFavoretto,PatríciaVeasey,Elizabeth AnnMelo,Paulo César Tavares deeng2012-01-05T00:00:00Zoai:scielo:S0102-05362011000400017Revistahttp://cms.horticulturabrasileira.com.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||hortbras@gmail.com1806-99910102-0536opendoar:2012-01-05T00:00Horticultura Brasileira - Associação Brasileira de Horticultura (ABH)false
dc.title.none.fl_str_mv Molecular characterization of potato cultivars using SSR markers
title Molecular characterization of potato cultivars using SSR markers
spellingShingle Molecular characterization of potato cultivars using SSR markers
Favoretto,Patrícia
Solanum tuberosum
germplasm banks
genetic diversity
microsatellites
title_short Molecular characterization of potato cultivars using SSR markers
title_full Molecular characterization of potato cultivars using SSR markers
title_fullStr Molecular characterization of potato cultivars using SSR markers
title_full_unstemmed Molecular characterization of potato cultivars using SSR markers
title_sort Molecular characterization of potato cultivars using SSR markers
author Favoretto,Patrícia
author_facet Favoretto,Patrícia
Veasey,Elizabeth Ann
Melo,Paulo César Tavares de
author_role author
author2 Veasey,Elizabeth Ann
Melo,Paulo César Tavares de
author2_role author
author
dc.contributor.author.fl_str_mv Favoretto,Patrícia
Veasey,Elizabeth Ann
Melo,Paulo César Tavares de
dc.subject.por.fl_str_mv Solanum tuberosum
germplasm banks
genetic diversity
microsatellites
topic Solanum tuberosum
germplasm banks
genetic diversity
microsatellites
description The potato crop has a very narrow genetic base, so the use of molecular markers is a very important tool in the characterization of germplasm banks and evaluation of genetic divergence. The objective of this study was to identify, using microsatellite or simple sequence repeat (SSR) markers, 38 accessions of potato from two collections of commercial cultivars. For the molecular characterization 10 loci were used, generating a total of 46 alleles, which were analyzed as binary data. A cluster analysis was performed with the Jaccard´s similarity coefficient and the UPGMA method, using the software NTSYSpc. On average, the number of alleles per locus was 4.6, ranging from two alleles for primers STM1049, STM 1053 and STM 1104 to 12 alleles per locus for primer STM0019a. Of the 46 alleles, only five were monomorphic, therefore presenting 89.1% polymorphism. The polymorphism information content (PIC) varied from 0.13 to 0.86, with an average of 0.54. The Jaccard´s coefficient varied from 0.41 to 0.93, showing high genetic variability among accessions. Two possible duplicates [Atlantic (Canada) and Atlantic (Chile), and Colorado and Ágata (EPAMIG) (duplicates with these SSRs, which did not separate them)] were identified. High similarity was also shown by cultivars Chipie and Melodie (EPAMIG), Voyager and Gourmandine (EPAMIG), Eole and Caesar (EPAMIG), and Cupido and Santé (Pirassu). The most genetically divergent accessions (Lady Rosetta and HPC-7B) were also identified.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000400017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000400017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-05362011000400017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Horticultura
publisher.none.fl_str_mv Associação Brasileira de Horticultura
dc.source.none.fl_str_mv Horticultura Brasileira v.29 n.4 2011
reponame:Horticultura Brasileira
instname:Associação Brasileira de Horticultura (ABH)
instacron:ABH
instname_str Associação Brasileira de Horticultura (ABH)
instacron_str ABH
institution ABH
reponame_str Horticultura Brasileira
collection Horticultura Brasileira
repository.name.fl_str_mv Horticultura Brasileira - Associação Brasileira de Horticultura (ABH)
repository.mail.fl_str_mv ||hortbras@gmail.com
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