Molecular characterization of potato cultivars using SSR markers
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Horticultura Brasileira |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000400017 |
Resumo: | The potato crop has a very narrow genetic base, so the use of molecular markers is a very important tool in the characterization of germplasm banks and evaluation of genetic divergence. The objective of this study was to identify, using microsatellite or simple sequence repeat (SSR) markers, 38 accessions of potato from two collections of commercial cultivars. For the molecular characterization 10 loci were used, generating a total of 46 alleles, which were analyzed as binary data. A cluster analysis was performed with the Jaccard´s similarity coefficient and the UPGMA method, using the software NTSYSpc. On average, the number of alleles per locus was 4.6, ranging from two alleles for primers STM1049, STM 1053 and STM 1104 to 12 alleles per locus for primer STM0019a. Of the 46 alleles, only five were monomorphic, therefore presenting 89.1% polymorphism. The polymorphism information content (PIC) varied from 0.13 to 0.86, with an average of 0.54. The Jaccard´s coefficient varied from 0.41 to 0.93, showing high genetic variability among accessions. Two possible duplicates [Atlantic (Canada) and Atlantic (Chile), and Colorado and Ágata (EPAMIG) (duplicates with these SSRs, which did not separate them)] were identified. High similarity was also shown by cultivars Chipie and Melodie (EPAMIG), Voyager and Gourmandine (EPAMIG), Eole and Caesar (EPAMIG), and Cupido and Santé (Pirassu). The most genetically divergent accessions (Lady Rosetta and HPC-7B) were also identified. |
id |
ABH-1_89e90797659cd34a5baa6ac7f9f486fc |
---|---|
oai_identifier_str |
oai:scielo:S0102-05362011000400017 |
network_acronym_str |
ABH-1 |
network_name_str |
Horticultura Brasileira |
repository_id_str |
|
spelling |
Molecular characterization of potato cultivars using SSR markersSolanum tuberosumgermplasm banksgenetic diversitymicrosatellitesThe potato crop has a very narrow genetic base, so the use of molecular markers is a very important tool in the characterization of germplasm banks and evaluation of genetic divergence. The objective of this study was to identify, using microsatellite or simple sequence repeat (SSR) markers, 38 accessions of potato from two collections of commercial cultivars. For the molecular characterization 10 loci were used, generating a total of 46 alleles, which were analyzed as binary data. A cluster analysis was performed with the Jaccard´s similarity coefficient and the UPGMA method, using the software NTSYSpc. On average, the number of alleles per locus was 4.6, ranging from two alleles for primers STM1049, STM 1053 and STM 1104 to 12 alleles per locus for primer STM0019a. Of the 46 alleles, only five were monomorphic, therefore presenting 89.1% polymorphism. The polymorphism information content (PIC) varied from 0.13 to 0.86, with an average of 0.54. The Jaccard´s coefficient varied from 0.41 to 0.93, showing high genetic variability among accessions. Two possible duplicates [Atlantic (Canada) and Atlantic (Chile), and Colorado and Ágata (EPAMIG) (duplicates with these SSRs, which did not separate them)] were identified. High similarity was also shown by cultivars Chipie and Melodie (EPAMIG), Voyager and Gourmandine (EPAMIG), Eole and Caesar (EPAMIG), and Cupido and Santé (Pirassu). The most genetically divergent accessions (Lady Rosetta and HPC-7B) were also identified.Associação Brasileira de Horticultura2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000400017Horticultura Brasileira v.29 n.4 2011reponame:Horticultura Brasileirainstname:Associação Brasileira de Horticultura (ABH)instacron:ABH10.1590/S0102-05362011000400017info:eu-repo/semantics/openAccessFavoretto,PatríciaVeasey,Elizabeth AnnMelo,Paulo César Tavares deeng2012-01-05T00:00:00Zoai:scielo:S0102-05362011000400017Revistahttp://cms.horticulturabrasileira.com.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||hortbras@gmail.com1806-99910102-0536opendoar:2012-01-05T00:00Horticultura Brasileira - Associação Brasileira de Horticultura (ABH)false |
dc.title.none.fl_str_mv |
Molecular characterization of potato cultivars using SSR markers |
title |
Molecular characterization of potato cultivars using SSR markers |
spellingShingle |
Molecular characterization of potato cultivars using SSR markers Favoretto,Patrícia Solanum tuberosum germplasm banks genetic diversity microsatellites |
title_short |
Molecular characterization of potato cultivars using SSR markers |
title_full |
Molecular characterization of potato cultivars using SSR markers |
title_fullStr |
Molecular characterization of potato cultivars using SSR markers |
title_full_unstemmed |
Molecular characterization of potato cultivars using SSR markers |
title_sort |
Molecular characterization of potato cultivars using SSR markers |
author |
Favoretto,Patrícia |
author_facet |
Favoretto,Patrícia Veasey,Elizabeth Ann Melo,Paulo César Tavares de |
author_role |
author |
author2 |
Veasey,Elizabeth Ann Melo,Paulo César Tavares de |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Favoretto,Patrícia Veasey,Elizabeth Ann Melo,Paulo César Tavares de |
dc.subject.por.fl_str_mv |
Solanum tuberosum germplasm banks genetic diversity microsatellites |
topic |
Solanum tuberosum germplasm banks genetic diversity microsatellites |
description |
The potato crop has a very narrow genetic base, so the use of molecular markers is a very important tool in the characterization of germplasm banks and evaluation of genetic divergence. The objective of this study was to identify, using microsatellite or simple sequence repeat (SSR) markers, 38 accessions of potato from two collections of commercial cultivars. For the molecular characterization 10 loci were used, generating a total of 46 alleles, which were analyzed as binary data. A cluster analysis was performed with the Jaccard´s similarity coefficient and the UPGMA method, using the software NTSYSpc. On average, the number of alleles per locus was 4.6, ranging from two alleles for primers STM1049, STM 1053 and STM 1104 to 12 alleles per locus for primer STM0019a. Of the 46 alleles, only five were monomorphic, therefore presenting 89.1% polymorphism. The polymorphism information content (PIC) varied from 0.13 to 0.86, with an average of 0.54. The Jaccard´s coefficient varied from 0.41 to 0.93, showing high genetic variability among accessions. Two possible duplicates [Atlantic (Canada) and Atlantic (Chile), and Colorado and Ágata (EPAMIG) (duplicates with these SSRs, which did not separate them)] were identified. High similarity was also shown by cultivars Chipie and Melodie (EPAMIG), Voyager and Gourmandine (EPAMIG), Eole and Caesar (EPAMIG), and Cupido and Santé (Pirassu). The most genetically divergent accessions (Lady Rosetta and HPC-7B) were also identified. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000400017 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000400017 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-05362011000400017 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Horticultura |
publisher.none.fl_str_mv |
Associação Brasileira de Horticultura |
dc.source.none.fl_str_mv |
Horticultura Brasileira v.29 n.4 2011 reponame:Horticultura Brasileira instname:Associação Brasileira de Horticultura (ABH) instacron:ABH |
instname_str |
Associação Brasileira de Horticultura (ABH) |
instacron_str |
ABH |
institution |
ABH |
reponame_str |
Horticultura Brasileira |
collection |
Horticultura Brasileira |
repository.name.fl_str_mv |
Horticultura Brasileira - Associação Brasileira de Horticultura (ABH) |
repository.mail.fl_str_mv |
||hortbras@gmail.com |
_version_ |
1754213081198624768 |