Characterization of potato genotypes using molecular markers

Detalhes bibliográficos
Autor(a) principal: Collares, Eliana Antonia Silveira
Data de Publicação: 2004
Outros Autores: Choer, Eva, Pereira, Arione da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/6851
Resumo: The objective of this work was to characterize 27 potato genotypes, using molecular markers. Polyacrylamide gel electrophoresis, RAPD techniques and isozymes of esterase, phosphoglucomutase and soluble proteins were analyzed in tubers, and isocitrate dehydrogenase, aspartate transaminase, phosphoglucomutase and peroxidase, in leaves. Eighteen primers were tested and four were chosen, kits OPX (01, 04 and 09) and OPY (07), to analyze RAPD markers in leaf extracts. Similarity and cluster analysis were conducted using Jaccard coefficient and the unweighted pair-group method using arithmetic average. Despite the differences detected in the analysis of proteins and isozymes in the tubers, as well as of isozymes in the leaves, the characterization of all genotypes through gel electrophoresis was not possible, while RAPD markers were efficient to characterize all the 27 genotypes.
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spelling Characterization of potato genotypes using molecular markersCaracterização de genótipos de batata por marcadores molecularesSolanum tuberosum; electrophoresis; isozymes; RAPD; genetic similaritySolanum tuberosum; eletroforese; isoenzimas; RAPD; similaridade genéticaThe objective of this work was to characterize 27 potato genotypes, using molecular markers. Polyacrylamide gel electrophoresis, RAPD techniques and isozymes of esterase, phosphoglucomutase and soluble proteins were analyzed in tubers, and isocitrate dehydrogenase, aspartate transaminase, phosphoglucomutase and peroxidase, in leaves. Eighteen primers were tested and four were chosen, kits OPX (01, 04 and 09) and OPY (07), to analyze RAPD markers in leaf extracts. Similarity and cluster analysis were conducted using Jaccard coefficient and the unweighted pair-group method using arithmetic average. Despite the differences detected in the analysis of proteins and isozymes in the tubers, as well as of isozymes in the leaves, the characterization of all genotypes through gel electrophoresis was not possible, while RAPD markers were efficient to characterize all the 27 genotypes.O objetivo deste trabalho foi caracterizar, por meio de marcadores moleculares, 27 genótipos de batata. Foram empregadas técnicas de eletroforese em gel de poliacrilamida e RAPD, e analisadas isoenzimas de esterase, fosfoglucomutase e proteína em tubérculos, isocitrato desidrogenase, aspartato transaminase, fosfoglucomutase e peroxidase, em folhas. Dos 18 primers testados, foram selecionados quatro dos kits OPX (01, 04 e 09) e OPY (07) para análise de RAPD em folhas. As análises de similaridade e de agrupamento entre os genótipos foram feitas empregando-se o coeficiente de Jaccard e o método da média aritmética não ponderada. Apesar das diferenças observadas nas análises de proteína e de isoenzimas, em tubérculos e em folhas, não foi possível a caracterização de todos os genótipos por esta técnica, enquanto os marcadores RAPD mostram-se eficientes em caracterizar as cultivares e clones de batata.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCollares, Eliana Antonia SilveiraChoer, EvaPereira, Arione da Silva2004-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6851Pesquisa Agropecuaria Brasileira; v.39, n.9, set. 2004; 871-878Pesquisa Agropecuária Brasileira; v.39, n.9, set. 2004; 871-8781678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/6851/3907info:eu-repo/semantics/openAccess2014-08-26T18:02:38Zoai:ojs.seer.sct.embrapa.br:article/6851Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-08-26T18:02:38Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Characterization of potato genotypes using molecular markers
Caracterização de genótipos de batata por marcadores moleculares
title Characterization of potato genotypes using molecular markers
spellingShingle Characterization of potato genotypes using molecular markers
Collares, Eliana Antonia Silveira
Solanum tuberosum; electrophoresis; isozymes; RAPD; genetic similarity
Solanum tuberosum; eletroforese; isoenzimas; RAPD; similaridade genética
title_short Characterization of potato genotypes using molecular markers
title_full Characterization of potato genotypes using molecular markers
title_fullStr Characterization of potato genotypes using molecular markers
title_full_unstemmed Characterization of potato genotypes using molecular markers
title_sort Characterization of potato genotypes using molecular markers
author Collares, Eliana Antonia Silveira
author_facet Collares, Eliana Antonia Silveira
Choer, Eva
Pereira, Arione da Silva
author_role author
author2 Choer, Eva
Pereira, Arione da Silva
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Collares, Eliana Antonia Silveira
Choer, Eva
Pereira, Arione da Silva
dc.subject.por.fl_str_mv Solanum tuberosum; electrophoresis; isozymes; RAPD; genetic similarity
Solanum tuberosum; eletroforese; isoenzimas; RAPD; similaridade genética
topic Solanum tuberosum; electrophoresis; isozymes; RAPD; genetic similarity
Solanum tuberosum; eletroforese; isoenzimas; RAPD; similaridade genética
description The objective of this work was to characterize 27 potato genotypes, using molecular markers. Polyacrylamide gel electrophoresis, RAPD techniques and isozymes of esterase, phosphoglucomutase and soluble proteins were analyzed in tubers, and isocitrate dehydrogenase, aspartate transaminase, phosphoglucomutase and peroxidase, in leaves. Eighteen primers were tested and four were chosen, kits OPX (01, 04 and 09) and OPY (07), to analyze RAPD markers in leaf extracts. Similarity and cluster analysis were conducted using Jaccard coefficient and the unweighted pair-group method using arithmetic average. Despite the differences detected in the analysis of proteins and isozymes in the tubers, as well as of isozymes in the leaves, the characterization of all genotypes through gel electrophoresis was not possible, while RAPD markers were efficient to characterize all the 27 genotypes.
publishDate 2004
dc.date.none.fl_str_mv 2004-09-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6851
url https://seer.sct.embrapa.br/index.php/pab/article/view/6851
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6851/3907
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.39, n.9, set. 2004; 871-878
Pesquisa Agropecuária Brasileira; v.39, n.9, set. 2004; 871-878
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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