Genomic instability in patients with type 2 diabetes mellitus on hemodialysis

Detalhes bibliográficos
Autor(a) principal: Palazzo,Roberta Passos
Data de Publicação: 2012
Outros Autores: Bagatini,Pamela Brambilla, Schefer,Patrícia Brandt, Andrade,Fabiana Michelsen de, Maluf,Sharbel Weidner
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista brasileira de hematologia e hemoterapia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842012000100011
Resumo: OBJECTIVE: A previous study by our research group evaluated the levels of DNA damage using the comet assay in hemodialysis patients with type 2 diabetes mellitus. The same blood samples were also evaluated using the cytochalasin B micronucleus assay. A comparison of the results of the two assays is presented here. METHODS: Whole blood samples were collected from 22 type 2 diabetes mellitus patients on hemodialysis and from 22 control subjects. Samples were collected from patients early in the morning on Mondays, before the first weekly hemodialysis session. The cytokinesis-block micronucleus assay (CBMN) was used to evaluate genomic instability. RESULTS: The frequencies of micronuclei and nuclear buds were higher in patients than in controls (p-value = 0.001 and p-value < 0.001, respectively). There was a correlation between the frequency of micronuclei and DNA damage with the results of the comet assay (p-value < 0.001). The difference in the frequency of micronuclei and nuclear buds between patients and controls was more pronounced in the group with higher median comet values than in the group with lower comet values. CONCLUSIONS: Our results suggest that the increased rates of DNA damage as measured by the comet assay and influenced by the weekly routine therapy of these patients has a mutagenic effect, thereby increasing the risk of cancer in this group.
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spelling Genomic instability in patients with type 2 diabetes mellitus on hemodialysisDiabetes Mellitus, type 2Micronucleus testsComet assayGenomic instabilityRenal dialysisOBJECTIVE: A previous study by our research group evaluated the levels of DNA damage using the comet assay in hemodialysis patients with type 2 diabetes mellitus. The same blood samples were also evaluated using the cytochalasin B micronucleus assay. A comparison of the results of the two assays is presented here. METHODS: Whole blood samples were collected from 22 type 2 diabetes mellitus patients on hemodialysis and from 22 control subjects. Samples were collected from patients early in the morning on Mondays, before the first weekly hemodialysis session. The cytokinesis-block micronucleus assay (CBMN) was used to evaluate genomic instability. RESULTS: The frequencies of micronuclei and nuclear buds were higher in patients than in controls (p-value = 0.001 and p-value < 0.001, respectively). There was a correlation between the frequency of micronuclei and DNA damage with the results of the comet assay (p-value < 0.001). The difference in the frequency of micronuclei and nuclear buds between patients and controls was more pronounced in the group with higher median comet values than in the group with lower comet values. CONCLUSIONS: Our results suggest that the increased rates of DNA damage as measured by the comet assay and influenced by the weekly routine therapy of these patients has a mutagenic effect, thereby increasing the risk of cancer in this group.Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842012000100011Revista Brasileira de Hematologia e Hemoterapia v.34 n.1 2012reponame:Revista brasileira de hematologia e hemoterapia (Online)instname:Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC)instacron:ABHHTC10.5581/1516-8484.20120011info:eu-repo/semantics/openAccessPalazzo,Roberta PassosBagatini,Pamela BrambillaSchefer,Patrícia BrandtAndrade,Fabiana Michelsen deMaluf,Sharbel Weidnereng2012-03-13T00:00:00Zoai:scielo:S1516-84842012000100011Revistahttp://www.rbhh.org/pt/archivo/https://old.scielo.br/oai/scielo-oai.phpsbhh@terra.com.br||secretaria@rbhh.org1806-08701516-8484opendoar:2012-03-13T00:00Revista brasileira de hematologia e hemoterapia (Online) - Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC)false
dc.title.none.fl_str_mv Genomic instability in patients with type 2 diabetes mellitus on hemodialysis
title Genomic instability in patients with type 2 diabetes mellitus on hemodialysis
spellingShingle Genomic instability in patients with type 2 diabetes mellitus on hemodialysis
Palazzo,Roberta Passos
Diabetes Mellitus, type 2
Micronucleus tests
Comet assay
Genomic instability
Renal dialysis
title_short Genomic instability in patients with type 2 diabetes mellitus on hemodialysis
title_full Genomic instability in patients with type 2 diabetes mellitus on hemodialysis
title_fullStr Genomic instability in patients with type 2 diabetes mellitus on hemodialysis
title_full_unstemmed Genomic instability in patients with type 2 diabetes mellitus on hemodialysis
title_sort Genomic instability in patients with type 2 diabetes mellitus on hemodialysis
author Palazzo,Roberta Passos
author_facet Palazzo,Roberta Passos
Bagatini,Pamela Brambilla
Schefer,Patrícia Brandt
Andrade,Fabiana Michelsen de
Maluf,Sharbel Weidner
author_role author
author2 Bagatini,Pamela Brambilla
Schefer,Patrícia Brandt
Andrade,Fabiana Michelsen de
Maluf,Sharbel Weidner
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Palazzo,Roberta Passos
Bagatini,Pamela Brambilla
Schefer,Patrícia Brandt
Andrade,Fabiana Michelsen de
Maluf,Sharbel Weidner
dc.subject.por.fl_str_mv Diabetes Mellitus, type 2
Micronucleus tests
Comet assay
Genomic instability
Renal dialysis
topic Diabetes Mellitus, type 2
Micronucleus tests
Comet assay
Genomic instability
Renal dialysis
description OBJECTIVE: A previous study by our research group evaluated the levels of DNA damage using the comet assay in hemodialysis patients with type 2 diabetes mellitus. The same blood samples were also evaluated using the cytochalasin B micronucleus assay. A comparison of the results of the two assays is presented here. METHODS: Whole blood samples were collected from 22 type 2 diabetes mellitus patients on hemodialysis and from 22 control subjects. Samples were collected from patients early in the morning on Mondays, before the first weekly hemodialysis session. The cytokinesis-block micronucleus assay (CBMN) was used to evaluate genomic instability. RESULTS: The frequencies of micronuclei and nuclear buds were higher in patients than in controls (p-value = 0.001 and p-value < 0.001, respectively). There was a correlation between the frequency of micronuclei and DNA damage with the results of the comet assay (p-value < 0.001). The difference in the frequency of micronuclei and nuclear buds between patients and controls was more pronounced in the group with higher median comet values than in the group with lower comet values. CONCLUSIONS: Our results suggest that the increased rates of DNA damage as measured by the comet assay and influenced by the weekly routine therapy of these patients has a mutagenic effect, thereby increasing the risk of cancer in this group.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842012000100011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842012000100011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5581/1516-8484.20120011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular
publisher.none.fl_str_mv Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular
dc.source.none.fl_str_mv Revista Brasileira de Hematologia e Hemoterapia v.34 n.1 2012
reponame:Revista brasileira de hematologia e hemoterapia (Online)
instname:Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC)
instacron:ABHHTC
instname_str Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC)
instacron_str ABHHTC
institution ABHHTC
reponame_str Revista brasileira de hematologia e hemoterapia (Online)
collection Revista brasileira de hematologia e hemoterapia (Online)
repository.name.fl_str_mv Revista brasileira de hematologia e hemoterapia (Online) - Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC)
repository.mail.fl_str_mv sbhh@terra.com.br||secretaria@rbhh.org
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