The Effect of Alcohol Consumption on the Development of Periapical Lesion Induced in Rats: a Micro-CT Analysis

• Autor(a) principal: Guimarães, Alexandre de Freitas Caetano
• Data de Publicação: 2020
• Outros Autores: Pinto, Karem Paula, Ferreira, Cláudio Malizia Alves, Lima, Carolina Oliveira de, Sassone, Luciana Moura, Silva, Emmanuel João Nogueira Leal da
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista Brasileira de Odontologia
• Texto Completo: https://revista.aborj.org.br/index.php/rbo/article/view/1859
• topic: DENTAL MEDICINE (DENTISTRY); Alcoholism; Micro-computed tomography; Osteoclastogenesis; Periapical periodontitis; endodontics; MEDICINA DENTÁRIA (ODONTOLOGIA); Alcoolismo; Microtomografia por raio-x; Osteoclastogênese; Periodontite periapical.; Endodontia

Objective: the aim of this study was to evaluate the influence of alcohol consumption on the increase of periapical bone destruction in rats. Material and Methods: the sample included 12 Wistar male rats, randomly assigned into a control group and an alcohol group (n=6). Rats in the alcohol group were submitted to self-administration of a 25% pure alcoholic solution. The control group received only filtered water throughout the study. After 5 weeks of adaptation to the alcohol dose, all animals were anesthetized and the pulps of their mandibular left first molar were exposed to the oral cavity to induce periapical lesion. Twenty-eight days after the pulp exposure, those rats were euthanized due to overdose of anesthesia and their mandibles were removed and sectioned to obtain a micro-computed tomographic (micro-CT) scan. The rats’ left hemimandibles were fixed and scanned on the SkyScan 1173 (Bruker, Konitch, Belgium) microtomograph. The size of the periradicular lesions was measured from the images obtained on the micro-CT and the surface area and volume were calculated. It was also evaluated the weight gain rate and the ingestion of solid/liquid of both groups. Data were analyzed by the Student’s t-test (p