Effects of different alcohol concentrations on the development of apical periodontitis in rats
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2019.104538 http://hdl.handle.net/11449/190614 |
Resumo: | Aim: To investigate the effect of different alcohol concentrations on the development of apical periodontitis (AP) in rats. Methods: Forty Wistar rats were arranged into five groups: (C) – control rats receiving sterile water as the only liquid; (G5) – animals receiving an alcohol solution at 5%, (G10) – alcohol solution at 10%, (G15) – alcohol solution at 15%, and (G20) – alcohol solution at 20%. The alcoholic solution or water was given to the groups as the sole source of hydration throughout the 30 days of the experiment. AP was induced in the mandibular molars on the first day. In the end, the animals were euthanized for histopathological and IL-1b, RANKL, OPG, and TRAP analyses. The Kruskal-Wallis test was used for nonparametric data, and ANOVA followed by the Tukey test were performed for parametric data, p < 0.05. Results: G15 and G20 had a greater chronic inflammatory infiltrate (Score 3) and AP size bigger (1.59 ± 0.41 and 1.83 ± 0.38, respectively) than the C, G5 and G10 (p < 0.05). No significant difference was found in the IL-1b analyses. The G15 and G20 showed the highest immunolabeling pattern for RANKL and the lowest for OPG. The G20 had greater TRAP cells per mm (4.70 ± 0.99) compared to the C, G5, and G10 (p < 0.05). Furthermore, G15 presented 3.92 ± 0.64 TRAP cells/mm, higher than C (p < 0.05). Conclusions: G5 and G10 did not exert a protective or aggravating effect on the AP development. However, G15 and G20 had a significant effect on the AP severity, exacerbating the inflammation and osteoclast markers. |
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Effects of different alcohol concentrations on the development of apical periodontitis in ratsAlcoholismEndodonticsPeriapical periodontitisAim: To investigate the effect of different alcohol concentrations on the development of apical periodontitis (AP) in rats. Methods: Forty Wistar rats were arranged into five groups: (C) – control rats receiving sterile water as the only liquid; (G5) – animals receiving an alcohol solution at 5%, (G10) – alcohol solution at 10%, (G15) – alcohol solution at 15%, and (G20) – alcohol solution at 20%. The alcoholic solution or water was given to the groups as the sole source of hydration throughout the 30 days of the experiment. AP was induced in the mandibular molars on the first day. In the end, the animals were euthanized for histopathological and IL-1b, RANKL, OPG, and TRAP analyses. The Kruskal-Wallis test was used for nonparametric data, and ANOVA followed by the Tukey test were performed for parametric data, p < 0.05. Results: G15 and G20 had a greater chronic inflammatory infiltrate (Score 3) and AP size bigger (1.59 ± 0.41 and 1.83 ± 0.38, respectively) than the C, G5 and G10 (p < 0.05). No significant difference was found in the IL-1b analyses. The G15 and G20 showed the highest immunolabeling pattern for RANKL and the lowest for OPG. The G20 had greater TRAP cells per mm (4.70 ± 0.99) compared to the C, G5, and G10 (p < 0.05). Furthermore, G15 presented 3.92 ± 0.64 TRAP cells/mm, higher than C (p < 0.05). Conclusions: G5 and G10 did not exert a protective or aggravating effect on the AP development. However, G15 and G20 had a significant effect on the AP severity, exacerbating the inflammation and osteoclast markers.Department of Endodontics São Paulo State University (Unesp) – School of DentistryDepartment of Basic Sciences São Paulo State University (Unesp) – School of DentistryDepartment of Restorative Dentistry Endodontics School of Dentistry Federal University of AlagoasDepartment of Endodontics São Paulo State University (Unesp) – School of DentistryDepartment of Basic Sciences São Paulo State University (Unesp) – School of DentistryUniversidade Estadual Paulista (Unesp)Federal University of AlagoasDal Fabbro, Renan [UNESP]Marques-de-Almeida, Melyna [UNESP]Cosme-Silva, Leopoldo [UNESP]Capalbo, Letícia Cabrera [UNESP]Ervolino, Edilson [UNESP]Cintra, Luciano Tavares Angelo [UNESP]Gomes Filho, João Eduardo [UNESP]2019-10-06T17:19:10Z2019-10-06T17:19:10Z2019-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.archoralbio.2019.104538Archives of Oral Biology, v. 108.1879-15060003-9969http://hdl.handle.net/11449/19061410.1016/j.archoralbio.2019.1045382-s2.0-85071434994440809551734684692357430816673620000-0003-4859-0583Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biologyinfo:eu-repo/semantics/openAccess2021-10-23T20:19:29Zoai:repositorio.unesp.br:11449/190614Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T20:19:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of different alcohol concentrations on the development of apical periodontitis in rats |
title |
Effects of different alcohol concentrations on the development of apical periodontitis in rats |
spellingShingle |
Effects of different alcohol concentrations on the development of apical periodontitis in rats Dal Fabbro, Renan [UNESP] Alcoholism Endodontics Periapical periodontitis |
title_short |
Effects of different alcohol concentrations on the development of apical periodontitis in rats |
title_full |
Effects of different alcohol concentrations on the development of apical periodontitis in rats |
title_fullStr |
Effects of different alcohol concentrations on the development of apical periodontitis in rats |
title_full_unstemmed |
Effects of different alcohol concentrations on the development of apical periodontitis in rats |
title_sort |
Effects of different alcohol concentrations on the development of apical periodontitis in rats |
author |
Dal Fabbro, Renan [UNESP] |
author_facet |
Dal Fabbro, Renan [UNESP] Marques-de-Almeida, Melyna [UNESP] Cosme-Silva, Leopoldo [UNESP] Capalbo, Letícia Cabrera [UNESP] Ervolino, Edilson [UNESP] Cintra, Luciano Tavares Angelo [UNESP] Gomes Filho, João Eduardo [UNESP] |
author_role |
author |
author2 |
Marques-de-Almeida, Melyna [UNESP] Cosme-Silva, Leopoldo [UNESP] Capalbo, Letícia Cabrera [UNESP] Ervolino, Edilson [UNESP] Cintra, Luciano Tavares Angelo [UNESP] Gomes Filho, João Eduardo [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Federal University of Alagoas |
dc.contributor.author.fl_str_mv |
Dal Fabbro, Renan [UNESP] Marques-de-Almeida, Melyna [UNESP] Cosme-Silva, Leopoldo [UNESP] Capalbo, Letícia Cabrera [UNESP] Ervolino, Edilson [UNESP] Cintra, Luciano Tavares Angelo [UNESP] Gomes Filho, João Eduardo [UNESP] |
dc.subject.por.fl_str_mv |
Alcoholism Endodontics Periapical periodontitis |
topic |
Alcoholism Endodontics Periapical periodontitis |
description |
Aim: To investigate the effect of different alcohol concentrations on the development of apical periodontitis (AP) in rats. Methods: Forty Wistar rats were arranged into five groups: (C) – control rats receiving sterile water as the only liquid; (G5) – animals receiving an alcohol solution at 5%, (G10) – alcohol solution at 10%, (G15) – alcohol solution at 15%, and (G20) – alcohol solution at 20%. The alcoholic solution or water was given to the groups as the sole source of hydration throughout the 30 days of the experiment. AP was induced in the mandibular molars on the first day. In the end, the animals were euthanized for histopathological and IL-1b, RANKL, OPG, and TRAP analyses. The Kruskal-Wallis test was used for nonparametric data, and ANOVA followed by the Tukey test were performed for parametric data, p < 0.05. Results: G15 and G20 had a greater chronic inflammatory infiltrate (Score 3) and AP size bigger (1.59 ± 0.41 and 1.83 ± 0.38, respectively) than the C, G5 and G10 (p < 0.05). No significant difference was found in the IL-1b analyses. The G15 and G20 showed the highest immunolabeling pattern for RANKL and the lowest for OPG. The G20 had greater TRAP cells per mm (4.70 ± 0.99) compared to the C, G5, and G10 (p < 0.05). Furthermore, G15 presented 3.92 ± 0.64 TRAP cells/mm, higher than C (p < 0.05). Conclusions: G5 and G10 did not exert a protective or aggravating effect on the AP development. However, G15 and G20 had a significant effect on the AP severity, exacerbating the inflammation and osteoclast markers. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T17:19:10Z 2019-10-06T17:19:10Z 2019-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2019.104538 Archives of Oral Biology, v. 108. 1879-1506 0003-9969 http://hdl.handle.net/11449/190614 10.1016/j.archoralbio.2019.104538 2-s2.0-85071434994 4408095517346846 9235743081667362 0000-0003-4859-0583 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2019.104538 http://hdl.handle.net/11449/190614 |
identifier_str_mv |
Archives of Oral Biology, v. 108. 1879-1506 0003-9969 10.1016/j.archoralbio.2019.104538 2-s2.0-85071434994 4408095517346846 9235743081667362 0000-0003-4859-0583 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965017008766976 |