Thin Layer chromatography as innovative technique for qualitative characterization of Biodiesel produced by esterification

• Autor(a) principal: Stracke, Marcelo Paulo
• Data de Publicação: 2019
• Outros Autores: dos Santos, Antonio Vanderlei, Marchetti, Mauro Cesar, da Cunha, Caroline Barlette, Brondani, Michel, Wancura, João Henrique Cabral, Mayer, Flávio Dias, Hoffmann, Ronaldo
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista GEINTEC: Gestão. Inovação e Tecnologias
• Texto Completo: http://www.revistageintec.net/index.php/revista/article/view/1221

The objective of this work was to evaluate the use of TLC, using the iodine vapor visualization method, for identification and preliminary characterization of biodiesel produced by esterification. The esterification reaction was performed at constant temperature of 65 °C for 2 hours, using dimethyl sulfate as alkylating agent and dodecanoic acid as oleaginous raw material. The chromatographic plate was subjected to the method of revelation after adding 5 µL of a sample of fatty acid and biodiesel on the chromatographic vessel. The indication of biodiesel formation was obtained by calculating the retention factor (Rf) and validated by hydrogen nuclear magnetic resonance (H1NMR). The Rf values and of the region of peaks of chemical functions were identical to those found in other studies, which allowed to confirm the formation of biodiesel. It can be concluded that TLC can be used as a innovative technique for preliminary qualitative characterization of biodiesel formation by esterification.