Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additives
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista GEINTEC: Gestão. Inovação e Tecnologias |
Texto Completo: | http://www.revistageintec.net/index.php/revista/article/view/1377 |
Resumo: | Enzymatic stability is undoubtedly one of the most important factors in biotechnology. In this sense, the stability of cellulases present in crude extracts of submerged cultures of Bacillus sp. SMIA-2 during freezing at 3 °C and in the presence of additives was studied. Cellulases were stable during storage for 154 days at 3 °C. The activity of avicellase and CMCase was maintained when crude extracts containing the enzymes were incubated in the presence of ethylene glycol (1M), glycerol (1%) and polyethylene glycol (0.01M) for 1 hour at room temperature. |
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Revista GEINTEC: Gestão. Inovação e Tecnologias |
spelling |
Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additivesEnzymatic stability is undoubtedly one of the most important factors in biotechnology. In this sense, the stability of cellulases present in crude extracts of submerged cultures of Bacillus sp. SMIA-2 during freezing at 3 °C and in the presence of additives was studied. Cellulases were stable during storage for 154 days at 3 °C. The activity of avicellase and CMCase was maintained when crude extracts containing the enzymes were incubated in the presence of ethylene glycol (1M), glycerol (1%) and polyethylene glycol (0.01M) for 1 hour at room temperature.API - Associação Acadêmica de Propriedade IntelectualCruz, EricaBarbosa, Joao BatistaTalma, Simone VilelaCosta, Edite AndradeMartins, Meire Lelis Leal2019-03-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttp://www.revistageintec.net/index.php/revista/article/view/137710.7198/geintec.v9i1.1377Revista GEINTEC - Gestão, Inovação e Tecnologias; v. 9, n. 1 (2019); 4740-47442237-0722reponame:Revista GEINTEC: Gestão. Inovação e Tecnologiasinstname:Ensino Superior do Piauí (AESPI)instacron:AESPIporhttp://www.revistageintec.net/index.php/revista/article/view/1377/894Direitos autorais 2019 Revista GEINTEC - Gestão, Inovação e Tecnologiashttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccess2019-10-06T00:05:22Zoai:ojs.pkp.sfu.ca:article/1377Revistahttp://www.revistageintec.net/index.php/revista/oai2237-07222237-0722opendoar:null2020-06-25 22:43:27.132Revista GEINTEC: Gestão. Inovação e Tecnologias - Ensino Superior do Piauí (AESPI)true |
dc.title.none.fl_str_mv |
Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additives |
title |
Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additives |
spellingShingle |
Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additives Cruz, Erica |
title_short |
Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additives |
title_full |
Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additives |
title_fullStr |
Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additives |
title_full_unstemmed |
Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additives |
title_sort |
Stability cellulases of Bacillus sp. SMIA-2 during storage under freezing and in the presence of additives |
author |
Cruz, Erica |
author_facet |
Cruz, Erica Barbosa, Joao Batista Talma, Simone Vilela Costa, Edite Andrade Martins, Meire Lelis Leal |
author_role |
author |
author2 |
Barbosa, Joao Batista Talma, Simone Vilela Costa, Edite Andrade Martins, Meire Lelis Leal |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Cruz, Erica Barbosa, Joao Batista Talma, Simone Vilela Costa, Edite Andrade Martins, Meire Lelis Leal |
dc.subject.none.fl_str_mv |
|
dc.description.none.fl_txt_mv |
Enzymatic stability is undoubtedly one of the most important factors in biotechnology. In this sense, the stability of cellulases present in crude extracts of submerged cultures of Bacillus sp. SMIA-2 during freezing at 3 °C and in the presence of additives was studied. Cellulases were stable during storage for 154 days at 3 °C. The activity of avicellase and CMCase was maintained when crude extracts containing the enzymes were incubated in the presence of ethylene glycol (1M), glycerol (1%) and polyethylene glycol (0.01M) for 1 hour at room temperature. |
description |
Enzymatic stability is undoubtedly one of the most important factors in biotechnology. In this sense, the stability of cellulases present in crude extracts of submerged cultures of Bacillus sp. SMIA-2 during freezing at 3 °C and in the presence of additives was studied. Cellulases were stable during storage for 154 days at 3 °C. The activity of avicellase and CMCase was maintained when crude extracts containing the enzymes were incubated in the presence of ethylene glycol (1M), glycerol (1%) and polyethylene glycol (0.01M) for 1 hour at room temperature. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-03-24 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.revistageintec.net/index.php/revista/article/view/1377 10.7198/geintec.v9i1.1377 |
url |
http://www.revistageintec.net/index.php/revista/article/view/1377 |
identifier_str_mv |
10.7198/geintec.v9i1.1377 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www.revistageintec.net/index.php/revista/article/view/1377/894 |
dc.rights.driver.fl_str_mv |
Direitos autorais 2019 Revista GEINTEC - Gestão, Inovação e Tecnologias https://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Direitos autorais 2019 Revista GEINTEC - Gestão, Inovação e Tecnologias https://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
API - Associação Acadêmica de Propriedade Intelectual |
publisher.none.fl_str_mv |
API - Associação Acadêmica de Propriedade Intelectual |
dc.source.none.fl_str_mv |
Revista GEINTEC - Gestão, Inovação e Tecnologias; v. 9, n. 1 (2019); 4740-4744 2237-0722 reponame:Revista GEINTEC: Gestão. Inovação e Tecnologias instname:Ensino Superior do Piauí (AESPI) instacron:AESPI |
reponame_str |
Revista GEINTEC: Gestão. Inovação e Tecnologias |
collection |
Revista GEINTEC: Gestão. Inovação e Tecnologias |
instname_str |
Ensino Superior do Piauí (AESPI) |
instacron_str |
AESPI |
institution |
AESPI |
repository.name.fl_str_mv |
Revista GEINTEC: Gestão. Inovação e Tecnologias - Ensino Superior do Piauí (AESPI) |
repository.mail.fl_str_mv |
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