Evaluation of the genotypic pattern of HIV-1 resistance in AIDS patients failing antiretroviral therapy

Detalhes bibliográficos
Autor(a) principal: Bahia,Fabianna
Data de Publicação: 2004
Outros Autores: Pedroso,Célia, Netto,Eduardo Martins, Figueiredo,Ricardo, Pinto Neto,Lauro, Brites,Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Infectious Diseases
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000400003
Resumo: We analyzed the first 96 patients tested for HIV resistance to antiretroviral therapy in three Brazilian states. The HIV-1 reverse transcriptase (RT) and protease (PR) were sequenced by using the ABI ViroSeq system. The drugs previously used for each patient were recorded and correlated with the mutations found in the samples. Viral load (VL) and CD4 count were also recorded. Only one patient had the wild type sequence. The most prevalent mutations were: 184V (59%), 41L (47.9%), 63P (53%), 215Y (50%), 36I (46%), 10I (35%), 67N (42%), 77I (37%), 90M (36%) and 210W (33%). A positive correlation between the number of previously used ARVs and the number of mutations was observed (p<0.05). Associations between mutations and ARV drugs were identified at positions 69, 118, 184 and 215 with previous exposure to NRTI, mutations at positions 98, 100, 103, 181 and 190 with previous NNRTI use and at positions 10, 20, 30, 46, 53, 54, 71, 73, 82, 84, 88 and 90 with previous PI therapy (p<0.05). Previous exposure to ARV drugs was associated with previous genotypic resistance to specific drugs, leading to treatment failure in HIV patients. Genotypic resistance was clearly associated with virological and immunological failure.
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spelling Evaluation of the genotypic pattern of HIV-1 resistance in AIDS patients failing antiretroviral therapyHIV-1mutationsresistanceantiretroviralsWe analyzed the first 96 patients tested for HIV resistance to antiretroviral therapy in three Brazilian states. The HIV-1 reverse transcriptase (RT) and protease (PR) were sequenced by using the ABI ViroSeq system. The drugs previously used for each patient were recorded and correlated with the mutations found in the samples. Viral load (VL) and CD4 count were also recorded. Only one patient had the wild type sequence. The most prevalent mutations were: 184V (59%), 41L (47.9%), 63P (53%), 215Y (50%), 36I (46%), 10I (35%), 67N (42%), 77I (37%), 90M (36%) and 210W (33%). A positive correlation between the number of previously used ARVs and the number of mutations was observed (p<0.05). Associations between mutations and ARV drugs were identified at positions 69, 118, 184 and 215 with previous exposure to NRTI, mutations at positions 98, 100, 103, 181 and 190 with previous NNRTI use and at positions 10, 20, 30, 46, 53, 54, 71, 73, 82, 84, 88 and 90 with previous PI therapy (p<0.05). Previous exposure to ARV drugs was associated with previous genotypic resistance to specific drugs, leading to treatment failure in HIV patients. Genotypic resistance was clearly associated with virological and immunological failure.Brazilian Society of Infectious Diseases2004-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000400003Brazilian Journal of Infectious Diseases v.8 n.4 2004reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1590/S1413-86702004000400003info:eu-repo/semantics/openAccessBahia,FabiannaPedroso,CéliaNetto,Eduardo MartinsFigueiredo,RicardoPinto Neto,LauroBrites,Carloseng2004-11-19T00:00:00Zoai:scielo:S1413-86702004000400003Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2004-11-19T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false
dc.title.none.fl_str_mv Evaluation of the genotypic pattern of HIV-1 resistance in AIDS patients failing antiretroviral therapy
title Evaluation of the genotypic pattern of HIV-1 resistance in AIDS patients failing antiretroviral therapy
spellingShingle Evaluation of the genotypic pattern of HIV-1 resistance in AIDS patients failing antiretroviral therapy
Bahia,Fabianna
HIV-1
mutations
resistance
antiretrovirals
title_short Evaluation of the genotypic pattern of HIV-1 resistance in AIDS patients failing antiretroviral therapy
title_full Evaluation of the genotypic pattern of HIV-1 resistance in AIDS patients failing antiretroviral therapy
title_fullStr Evaluation of the genotypic pattern of HIV-1 resistance in AIDS patients failing antiretroviral therapy
title_full_unstemmed Evaluation of the genotypic pattern of HIV-1 resistance in AIDS patients failing antiretroviral therapy
title_sort Evaluation of the genotypic pattern of HIV-1 resistance in AIDS patients failing antiretroviral therapy
author Bahia,Fabianna
author_facet Bahia,Fabianna
Pedroso,Célia
Netto,Eduardo Martins
Figueiredo,Ricardo
Pinto Neto,Lauro
Brites,Carlos
author_role author
author2 Pedroso,Célia
Netto,Eduardo Martins
Figueiredo,Ricardo
Pinto Neto,Lauro
Brites,Carlos
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Bahia,Fabianna
Pedroso,Célia
Netto,Eduardo Martins
Figueiredo,Ricardo
Pinto Neto,Lauro
Brites,Carlos
dc.subject.por.fl_str_mv HIV-1
mutations
resistance
antiretrovirals
topic HIV-1
mutations
resistance
antiretrovirals
description We analyzed the first 96 patients tested for HIV resistance to antiretroviral therapy in three Brazilian states. The HIV-1 reverse transcriptase (RT) and protease (PR) were sequenced by using the ABI ViroSeq system. The drugs previously used for each patient were recorded and correlated with the mutations found in the samples. Viral load (VL) and CD4 count were also recorded. Only one patient had the wild type sequence. The most prevalent mutations were: 184V (59%), 41L (47.9%), 63P (53%), 215Y (50%), 36I (46%), 10I (35%), 67N (42%), 77I (37%), 90M (36%) and 210W (33%). A positive correlation between the number of previously used ARVs and the number of mutations was observed (p<0.05). Associations between mutations and ARV drugs were identified at positions 69, 118, 184 and 215 with previous exposure to NRTI, mutations at positions 98, 100, 103, 181 and 190 with previous NNRTI use and at positions 10, 20, 30, 46, 53, 54, 71, 73, 82, 84, 88 and 90 with previous PI therapy (p<0.05). Previous exposure to ARV drugs was associated with previous genotypic resistance to specific drugs, leading to treatment failure in HIV patients. Genotypic resistance was clearly associated with virological and immunological failure.
publishDate 2004
dc.date.none.fl_str_mv 2004-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000400003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000400003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1413-86702004000400003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
dc.source.none.fl_str_mv Brazilian Journal of Infectious Diseases v.8 n.4 2004
reponame:Brazilian Journal of Infectious Diseases
instname:Brazilian Society of Infectious Diseases (BSID)
instacron:BSID
instname_str Brazilian Society of Infectious Diseases (BSID)
instacron_str BSID
institution BSID
reponame_str Brazilian Journal of Infectious Diseases
collection Brazilian Journal of Infectious Diseases
repository.name.fl_str_mv Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)
repository.mail.fl_str_mv bjid@bjid.org.br||lgoldani@ufrgs.br
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