Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Infectious Diseases |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100019 |
Resumo: | Abstract Background: Sepsis is an illness with a high morbidity for which no effective treatment exists. Its treatment has a high cost because it usually requires an intensive care unit and expensive antibiotics. The present study focus in the production of reactive oxygen species in the early stages of sepsis. This study aimed at investigating the production of reactive oxygen specie during the inflammatory response in patients with sepsis. Methods: Reactive oxygen specie production and insoluble myeloperoxidase obtained from fresh whole blood were measured by photon counting chemiluminescence in the blood of 18 septic patients and 12 healthy individuals. Modified red blood cells were evaluated by staining of blood smears. The production of reactive oxygen species by macrophages and polymorphonuclear leukocytes put into contact with modified red blood cells were also assessed by photon counting chemiluminescence. Results: The appearance of oxidatively modified erythrocytes, which is an evidence of oxidative stress, was supported by the detection of reactive oxygen species and insoluble myeloperoxidase in the whole blood of all septic patients. Peroxynitrite was the main reactive oxygen species found in the whole blood. Oxidatively modified erythrocytes activated phagocytic cells in vitro, leading to the considerable production of free radicals. Conclusion: It was found that sepsis led to a high oxidative stress and to extensive modification of erythrocytes. It is proposed that a positive feedback mechanism, involving the activation of circulating leukocytes by these modified erythrocytes would maintain the pro-oxidative state even after the disappearance of bacteria. |
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Brazilian Journal of Infectious Diseases |
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Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanismSepsisOxidative stressInflammationModified erythrocytesAbstract Background: Sepsis is an illness with a high morbidity for which no effective treatment exists. Its treatment has a high cost because it usually requires an intensive care unit and expensive antibiotics. The present study focus in the production of reactive oxygen species in the early stages of sepsis. This study aimed at investigating the production of reactive oxygen specie during the inflammatory response in patients with sepsis. Methods: Reactive oxygen specie production and insoluble myeloperoxidase obtained from fresh whole blood were measured by photon counting chemiluminescence in the blood of 18 septic patients and 12 healthy individuals. Modified red blood cells were evaluated by staining of blood smears. The production of reactive oxygen species by macrophages and polymorphonuclear leukocytes put into contact with modified red blood cells were also assessed by photon counting chemiluminescence. Results: The appearance of oxidatively modified erythrocytes, which is an evidence of oxidative stress, was supported by the detection of reactive oxygen species and insoluble myeloperoxidase in the whole blood of all septic patients. Peroxynitrite was the main reactive oxygen species found in the whole blood. Oxidatively modified erythrocytes activated phagocytic cells in vitro, leading to the considerable production of free radicals. Conclusion: It was found that sepsis led to a high oxidative stress and to extensive modification of erythrocytes. It is proposed that a positive feedback mechanism, involving the activation of circulating leukocytes by these modified erythrocytes would maintain the pro-oxidative state even after the disappearance of bacteria.Brazilian Society of Infectious Diseases2017-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100019Brazilian Journal of Infectious Diseases v.21 n.1 2017reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1016/j.bjid.2016.11.004info:eu-repo/semantics/openAccessOliveira,Yanaihara Pinchemel Amorim dePontes-de-Carvalho,Lain CarlosCouto,Ricardo DavidNoronha-Dutra,Alberto Augustoeng2017-03-06T00:00:00Zoai:scielo:S1413-86702017000100019Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2017-03-06T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false |
dc.title.none.fl_str_mv |
Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism |
title |
Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism |
spellingShingle |
Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism Oliveira,Yanaihara Pinchemel Amorim de Sepsis Oxidative stress Inflammation Modified erythrocytes |
title_short |
Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism |
title_full |
Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism |
title_fullStr |
Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism |
title_full_unstemmed |
Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism |
title_sort |
Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism |
author |
Oliveira,Yanaihara Pinchemel Amorim de |
author_facet |
Oliveira,Yanaihara Pinchemel Amorim de Pontes-de-Carvalho,Lain Carlos Couto,Ricardo David Noronha-Dutra,Alberto Augusto |
author_role |
author |
author2 |
Pontes-de-Carvalho,Lain Carlos Couto,Ricardo David Noronha-Dutra,Alberto Augusto |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Oliveira,Yanaihara Pinchemel Amorim de Pontes-de-Carvalho,Lain Carlos Couto,Ricardo David Noronha-Dutra,Alberto Augusto |
dc.subject.por.fl_str_mv |
Sepsis Oxidative stress Inflammation Modified erythrocytes |
topic |
Sepsis Oxidative stress Inflammation Modified erythrocytes |
description |
Abstract Background: Sepsis is an illness with a high morbidity for which no effective treatment exists. Its treatment has a high cost because it usually requires an intensive care unit and expensive antibiotics. The present study focus in the production of reactive oxygen species in the early stages of sepsis. This study aimed at investigating the production of reactive oxygen specie during the inflammatory response in patients with sepsis. Methods: Reactive oxygen specie production and insoluble myeloperoxidase obtained from fresh whole blood were measured by photon counting chemiluminescence in the blood of 18 septic patients and 12 healthy individuals. Modified red blood cells were evaluated by staining of blood smears. The production of reactive oxygen species by macrophages and polymorphonuclear leukocytes put into contact with modified red blood cells were also assessed by photon counting chemiluminescence. Results: The appearance of oxidatively modified erythrocytes, which is an evidence of oxidative stress, was supported by the detection of reactive oxygen species and insoluble myeloperoxidase in the whole blood of all septic patients. Peroxynitrite was the main reactive oxygen species found in the whole blood. Oxidatively modified erythrocytes activated phagocytic cells in vitro, leading to the considerable production of free radicals. Conclusion: It was found that sepsis led to a high oxidative stress and to extensive modification of erythrocytes. It is proposed that a positive feedback mechanism, involving the activation of circulating leukocytes by these modified erythrocytes would maintain the pro-oxidative state even after the disappearance of bacteria. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjid.2016.11.004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
dc.source.none.fl_str_mv |
Brazilian Journal of Infectious Diseases v.21 n.1 2017 reponame:Brazilian Journal of Infectious Diseases instname:Brazilian Society of Infectious Diseases (BSID) instacron:BSID |
instname_str |
Brazilian Society of Infectious Diseases (BSID) |
instacron_str |
BSID |
institution |
BSID |
reponame_str |
Brazilian Journal of Infectious Diseases |
collection |
Brazilian Journal of Infectious Diseases |
repository.name.fl_str_mv |
Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID) |
repository.mail.fl_str_mv |
bjid@bjid.org.br||lgoldani@ufrgs.br |
_version_ |
1754209244090990592 |