Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism

Detalhes bibliográficos
Autor(a) principal: Oliveira, Yanaihara Pinchemel Amorim de
Data de Publicação: 2017
Outros Autores: Pontes-de-Carvalho, Lain Carlos, Couto, Ricardo David, Dutra, Alberto Augusto Noronha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/17857
Resumo: FIOCRUZ-Bahia (Centro dePesquisas Gonçalo Moniz) for allowing the experiments whichneeded to be done, FAPESB for the financial support as well asthe Institutions and individuals who agreed to take part in thisProject.
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spelling Oliveira, Yanaihara Pinchemel Amorim dePontes-de-Carvalho, Lain CarlosCouto, Ricardo DavidDutra, Alberto Augusto Noronha2017-02-20T16:15:00Z2017-02-20T16:15:00Z2017OLIVEIRA, Y. P. A. et al. Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism. Brazilian Journal of Infectious Disease, v. 21, n. 1, p. 19–26, 2017.1413-8670https://www.arca.fiocruz.br/handle/icict/1785710.1016/j.bjid.2016.11.004FIOCRUZ-Bahia (Centro dePesquisas Gonçalo Moniz) for allowing the experiments whichneeded to be done, FAPESB for the financial support as well asthe Institutions and individuals who agreed to take part in thisProject.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilSepsis is an illness with a high morbidity for which no effective treatment exists. Its treatment has a high cost because it usually requires an intensive care unit and expensive antibiotics. The present study focus in the production of reactive oxygen species in the early stages of sepsis. This study aimed at investigating the production of reactive oxygen specie during the inflammatory response in patients with sepsis.Methods: Reactive oxygen specie production and insoluble myeloperoxidase obtained fromfresh whole blood were measured by photon counting chemiluminescence in the blood of18 septic patients and 12 healthy individuals. Modified red blood cells were evaluated bystaining of blood smears. The production of reactive oxygen species by macrophages andpolymorphonuclear leukocytes put into contact with modified red blood cells were alsoassessed by photon counting chemiluminescence.Results: The appearance of oxidatively modified erythrocytes, which is an evidence ofoxidative stress, was supported by the detection of reactive oxygen species and insolublemyeloperoxidase in the whole blood of all septic patients. Peroxynitrite was the main reac-tive oxygen species found in the whole blood. Oxidatively modified erythrocytes activatedphagocytic cells in vitro, leading to the considerable production of free radicals.Conclusion: It was found that sepsis led to a high oxidative stress and to extensive modifi-cation of erythrocytes. It is proposed that a positive feedback mechanism, involving theactivation of circulating leukocytes by these modified erythrocytes would maintain thepro-oxidative state even after the disappearance of bacteria.engElsevierSepsisEstresse oxidativoInflamaçãoEritrócitosSepsisOxidative stressInflammationModified erythrocytesaOxidative stress in sepsis. 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dc.title.pt_BR.fl_str_mv Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism
title Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism
spellingShingle Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism
Oliveira, Yanaihara Pinchemel Amorim de
Sepsis
Estresse oxidativo
Inflamação
Eritrócitos
Sepsis
Oxidative stress
Inflammation
Modified erythrocytesa
title_short Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism
title_full Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism
title_fullStr Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism
title_full_unstemmed Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism
title_sort Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism
author Oliveira, Yanaihara Pinchemel Amorim de
author_facet Oliveira, Yanaihara Pinchemel Amorim de
Pontes-de-Carvalho, Lain Carlos
Couto, Ricardo David
Dutra, Alberto Augusto Noronha
author_role author
author2 Pontes-de-Carvalho, Lain Carlos
Couto, Ricardo David
Dutra, Alberto Augusto Noronha
author2_role author
author
author
dc.contributor.author.fl_str_mv Oliveira, Yanaihara Pinchemel Amorim de
Pontes-de-Carvalho, Lain Carlos
Couto, Ricardo David
Dutra, Alberto Augusto Noronha
dc.subject.other.pt_BR.fl_str_mv Sepsis
Estresse oxidativo
Inflamação
Eritrócitos
topic Sepsis
Estresse oxidativo
Inflamação
Eritrócitos
Sepsis
Oxidative stress
Inflammation
Modified erythrocytesa
dc.subject.en.pt_BR.fl_str_mv Sepsis
Oxidative stress
Inflammation
Modified erythrocytesa
description FIOCRUZ-Bahia (Centro dePesquisas Gonçalo Moniz) for allowing the experiments whichneeded to be done, FAPESB for the financial support as well asthe Institutions and individuals who agreed to take part in thisProject.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-02-20T16:15:00Z
dc.date.available.fl_str_mv 2017-02-20T16:15:00Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Y. P. A. et al. Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism. Brazilian Journal of Infectious Disease, v. 21, n. 1, p. 19–26, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/17857
dc.identifier.issn.pt_BR.fl_str_mv 1413-8670
dc.identifier.doi.none.fl_str_mv 10.1016/j.bjid.2016.11.004
identifier_str_mv OLIVEIRA, Y. P. A. et al. Oxidative stress in sepsis. Possible production of free radicals through an erythrocyte-mediated positive feedback mechanism. Brazilian Journal of Infectious Disease, v. 21, n. 1, p. 19–26, 2017.
1413-8670
10.1016/j.bjid.2016.11.004
url https://www.arca.fiocruz.br/handle/icict/17857
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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