Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China

Detalhes bibliográficos
Autor(a) principal: Hou,Jinna
Data de Publicação: 2018
Outros Autores: An,Sufang, Liu,Yongjuan, Lei,Li, Li,Baoquan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000200155
Resumo: Abstract The lack of genetic diversity is an obstacle for genetic improvement of upland cotton in China; thus, new technologies must be developed to produce more polymorphic molecular markers associated with agricultural traits of the existing resources for breeding. A highly efficient and economical technology of sequence-related amplified polymorphism (SRAP) molecular markers with an automated fragment analyzer ABI3500xl was developed, to detect genetic diversity in upland cotton. Using this new strategy, we easily screened for polymorphisms with 7,872 pairs of SRAP primers, and detected 504 polymorphic markers. Of all these, 165 were used for genetic diversity analysis in 128 upland cotton varieties collected nationwide in China. Our method combined a traditional molecular marker development technology with an economical and easy operation strategy for breeders.
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spelling Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in ChinaSRAPgenetic diversityhigh efficient molecular markerupland cottonAbstract The lack of genetic diversity is an obstacle for genetic improvement of upland cotton in China; thus, new technologies must be developed to produce more polymorphic molecular markers associated with agricultural traits of the existing resources for breeding. A highly efficient and economical technology of sequence-related amplified polymorphism (SRAP) molecular markers with an automated fragment analyzer ABI3500xl was developed, to detect genetic diversity in upland cotton. Using this new strategy, we easily screened for polymorphisms with 7,872 pairs of SRAP primers, and detected 504 polymorphic markers. Of all these, 165 were used for genetic diversity analysis in 128 upland cotton varieties collected nationwide in China. Our method combined a traditional molecular marker development technology with an economical and easy operation strategy for breeders.Crop Breeding and Applied Biotechnology2018-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000200155Crop Breeding and Applied Biotechnology v.18 n.2 2018reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332018v18n2a22info:eu-repo/semantics/openAccessHou,JinnaAn,SufangLiu,YongjuanLei,LiLi,Baoquaneng2018-04-25T00:00:00Zoai:scielo:S1984-70332018000200155Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2018-04-25T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China
title Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China
spellingShingle Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China
Hou,Jinna
SRAP
genetic diversity
high efficient molecular marker
upland cotton
title_short Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China
title_full Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China
title_fullStr Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China
title_full_unstemmed Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China
title_sort Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China
author Hou,Jinna
author_facet Hou,Jinna
An,Sufang
Liu,Yongjuan
Lei,Li
Li,Baoquan
author_role author
author2 An,Sufang
Liu,Yongjuan
Lei,Li
Li,Baoquan
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Hou,Jinna
An,Sufang
Liu,Yongjuan
Lei,Li
Li,Baoquan
dc.subject.por.fl_str_mv SRAP
genetic diversity
high efficient molecular marker
upland cotton
topic SRAP
genetic diversity
high efficient molecular marker
upland cotton
description Abstract The lack of genetic diversity is an obstacle for genetic improvement of upland cotton in China; thus, new technologies must be developed to produce more polymorphic molecular markers associated with agricultural traits of the existing resources for breeding. A highly efficient and economical technology of sequence-related amplified polymorphism (SRAP) molecular markers with an automated fragment analyzer ABI3500xl was developed, to detect genetic diversity in upland cotton. Using this new strategy, we easily screened for polymorphisms with 7,872 pairs of SRAP primers, and detected 504 polymorphic markers. Of all these, 165 were used for genetic diversity analysis in 128 upland cotton varieties collected nationwide in China. Our method combined a traditional molecular marker development technology with an economical and easy operation strategy for breeders.
publishDate 2018
dc.date.none.fl_str_mv 2018-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000200155
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000200155
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332018v18n2a22
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.18 n.2 2018
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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