Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Crop Breeding and Applied Biotechnology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000200155 |
Resumo: | Abstract The lack of genetic diversity is an obstacle for genetic improvement of upland cotton in China; thus, new technologies must be developed to produce more polymorphic molecular markers associated with agricultural traits of the existing resources for breeding. A highly efficient and economical technology of sequence-related amplified polymorphism (SRAP) molecular markers with an automated fragment analyzer ABI3500xl was developed, to detect genetic diversity in upland cotton. Using this new strategy, we easily screened for polymorphisms with 7,872 pairs of SRAP primers, and detected 504 polymorphic markers. Of all these, 165 were used for genetic diversity analysis in 128 upland cotton varieties collected nationwide in China. Our method combined a traditional molecular marker development technology with an economical and easy operation strategy for breeders. |
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Crop Breeding and Applied Biotechnology |
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Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in ChinaSRAPgenetic diversityhigh efficient molecular markerupland cottonAbstract The lack of genetic diversity is an obstacle for genetic improvement of upland cotton in China; thus, new technologies must be developed to produce more polymorphic molecular markers associated with agricultural traits of the existing resources for breeding. A highly efficient and economical technology of sequence-related amplified polymorphism (SRAP) molecular markers with an automated fragment analyzer ABI3500xl was developed, to detect genetic diversity in upland cotton. Using this new strategy, we easily screened for polymorphisms with 7,872 pairs of SRAP primers, and detected 504 polymorphic markers. Of all these, 165 were used for genetic diversity analysis in 128 upland cotton varieties collected nationwide in China. Our method combined a traditional molecular marker development technology with an economical and easy operation strategy for breeders.Crop Breeding and Applied Biotechnology2018-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000200155Crop Breeding and Applied Biotechnology v.18 n.2 2018reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332018v18n2a22info:eu-repo/semantics/openAccessHou,JinnaAn,SufangLiu,YongjuanLei,LiLi,Baoquaneng2018-04-25T00:00:00Zoai:scielo:S1984-70332018000200155Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2018-04-25T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse |
dc.title.none.fl_str_mv |
Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China |
title |
Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China |
spellingShingle |
Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China Hou,Jinna SRAP genetic diversity high efficient molecular marker upland cotton |
title_short |
Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China |
title_full |
Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China |
title_fullStr |
Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China |
title_full_unstemmed |
Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China |
title_sort |
Sequence-related amplified polymorphisms (SRAPs) reveal genetic diversity and variation regions in upland cotton (Gossypium hirsutum L.) in China |
author |
Hou,Jinna |
author_facet |
Hou,Jinna An,Sufang Liu,Yongjuan Lei,Li Li,Baoquan |
author_role |
author |
author2 |
An,Sufang Liu,Yongjuan Lei,Li Li,Baoquan |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Hou,Jinna An,Sufang Liu,Yongjuan Lei,Li Li,Baoquan |
dc.subject.por.fl_str_mv |
SRAP genetic diversity high efficient molecular marker upland cotton |
topic |
SRAP genetic diversity high efficient molecular marker upland cotton |
description |
Abstract The lack of genetic diversity is an obstacle for genetic improvement of upland cotton in China; thus, new technologies must be developed to produce more polymorphic molecular markers associated with agricultural traits of the existing resources for breeding. A highly efficient and economical technology of sequence-related amplified polymorphism (SRAP) molecular markers with an automated fragment analyzer ABI3500xl was developed, to detect genetic diversity in upland cotton. Using this new strategy, we easily screened for polymorphisms with 7,872 pairs of SRAP primers, and detected 504 polymorphic markers. Of all these, 165 were used for genetic diversity analysis in 128 upland cotton varieties collected nationwide in China. Our method combined a traditional molecular marker development technology with an economical and easy operation strategy for breeders. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000200155 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332018000200155 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1984-70332018v18n2a22 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
dc.source.none.fl_str_mv |
Crop Breeding and Applied Biotechnology v.18 n.2 2018 reponame:Crop Breeding and Applied Biotechnology instname:Sociedade Brasileira de Melhoramento de Plantas instacron:CBAB |
instname_str |
Sociedade Brasileira de Melhoramento de Plantas |
instacron_str |
CBAB |
institution |
CBAB |
reponame_str |
Crop Breeding and Applied Biotechnology |
collection |
Crop Breeding and Applied Biotechnology |
repository.name.fl_str_mv |
Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas |
repository.mail.fl_str_mv |
cbabjournal@gmail.com||cbab@ufv.br |
_version_ |
1754209187562258432 |