Construction and characterization of a bacterial artificial chromosome library of the maize inbred line Qi319

Detalhes bibliográficos
Autor(a) principal: Mu,Chun Hua
Data de Publicação: 2016
Outros Autores: Yang,Yu, Zhang,Fa Jun, Li,Wen Cai, Lu,Shou Ping, Meng,Zhao Dong, Liu,Xia, Li,Guang Cun
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000100022
Resumo: Abstract Zea mays L. has been the most cultivated crop and the crop with the largest yield in China since 2012. We constructed a bacterial artificial chromosome (BAC) library for the maize inbred line Qi319, which may be used as a key source for disease-resistant maize breeding in China. The BAC contains 270,720 clones, with an average insert size of 90 kb. The coverage of the library is about 10.43 genome equivalents when considering a haploid genome size of 2300 Mb, providing a 99.99% likelihood of isolating any maize gene or sequence in the library. An average of 12 clones were obtained by polymerase chain reaction screening by using primer pairs linked to the genes for resistance to maize southern rust and rough dwarf. The results indicate that the library can satisfy the requirements for recovering specific sequences. The library is available to researchers to whom it may be of interest.
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spelling Construction and characterization of a bacterial artificial chromosome library of the maize inbred line Qi319Maizeinbred lineresistance genesbacterial artificial chromosome libraryAbstract Zea mays L. has been the most cultivated crop and the crop with the largest yield in China since 2012. We constructed a bacterial artificial chromosome (BAC) library for the maize inbred line Qi319, which may be used as a key source for disease-resistant maize breeding in China. The BAC contains 270,720 clones, with an average insert size of 90 kb. The coverage of the library is about 10.43 genome equivalents when considering a haploid genome size of 2300 Mb, providing a 99.99% likelihood of isolating any maize gene or sequence in the library. An average of 12 clones were obtained by polymerase chain reaction screening by using primer pairs linked to the genes for resistance to maize southern rust and rough dwarf. The results indicate that the library can satisfy the requirements for recovering specific sequences. The library is available to researchers to whom it may be of interest.Crop Breeding and Applied Biotechnology2016-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000100022Crop Breeding and Applied Biotechnology v.16 n.1 2016reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332016v16n1a4info:eu-repo/semantics/openAccessMu,Chun HuaYang,YuZhang,Fa JunLi,Wen CaiLu,Shou PingMeng,Zhao DongLiu,XiaLi,Guang Cuneng2016-04-19T00:00:00Zoai:scielo:S1984-70332016000100022Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2016-04-19T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Construction and characterization of a bacterial artificial chromosome library of the maize inbred line Qi319
title Construction and characterization of a bacterial artificial chromosome library of the maize inbred line Qi319
spellingShingle Construction and characterization of a bacterial artificial chromosome library of the maize inbred line Qi319
Mu,Chun Hua
Maize
inbred line
resistance genes
bacterial artificial chromosome library
title_short Construction and characterization of a bacterial artificial chromosome library of the maize inbred line Qi319
title_full Construction and characterization of a bacterial artificial chromosome library of the maize inbred line Qi319
title_fullStr Construction and characterization of a bacterial artificial chromosome library of the maize inbred line Qi319
title_full_unstemmed Construction and characterization of a bacterial artificial chromosome library of the maize inbred line Qi319
title_sort Construction and characterization of a bacterial artificial chromosome library of the maize inbred line Qi319
author Mu,Chun Hua
author_facet Mu,Chun Hua
Yang,Yu
Zhang,Fa Jun
Li,Wen Cai
Lu,Shou Ping
Meng,Zhao Dong
Liu,Xia
Li,Guang Cun
author_role author
author2 Yang,Yu
Zhang,Fa Jun
Li,Wen Cai
Lu,Shou Ping
Meng,Zhao Dong
Liu,Xia
Li,Guang Cun
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mu,Chun Hua
Yang,Yu
Zhang,Fa Jun
Li,Wen Cai
Lu,Shou Ping
Meng,Zhao Dong
Liu,Xia
Li,Guang Cun
dc.subject.por.fl_str_mv Maize
inbred line
resistance genes
bacterial artificial chromosome library
topic Maize
inbred line
resistance genes
bacterial artificial chromosome library
description Abstract Zea mays L. has been the most cultivated crop and the crop with the largest yield in China since 2012. We constructed a bacterial artificial chromosome (BAC) library for the maize inbred line Qi319, which may be used as a key source for disease-resistant maize breeding in China. The BAC contains 270,720 clones, with an average insert size of 90 kb. The coverage of the library is about 10.43 genome equivalents when considering a haploid genome size of 2300 Mb, providing a 99.99% likelihood of isolating any maize gene or sequence in the library. An average of 12 clones were obtained by polymerase chain reaction screening by using primer pairs linked to the genes for resistance to maize southern rust and rough dwarf. The results indicate that the library can satisfy the requirements for recovering specific sequences. The library is available to researchers to whom it may be of interest.
publishDate 2016
dc.date.none.fl_str_mv 2016-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000100022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000100022
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332016v16n1a4
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.16 n.1 2016
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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