Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean

Detalhes bibliográficos
Autor(a) principal: Morais,Samara Rayane Pereira de
Data de Publicação: 2016
Outros Autores: Vieira,Ariadna Faria, Almeida,Laura Cristina da Silva, Rodrigues,Luana Alves, Melo,Patrícia Guimarães Santos, Faria,Luís Cláudio de, Melo,Leonardo Cunha, Pereira,Helton Santos, Souza,Thiago Lívio Pessoa Oliveira de
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000300234
Resumo: Abstract This manuscript reports on the application of microsatellite markers to assess the effectiveness of controlled crosses and assess the genetic identity of seed samples from a same common bean cultivar (BRS Estilo). The DNA was extracted from leaf tissue collected from F1 plants and their parents as well as from seed samples of the cv. BRS Estilo by the alkaline lysis method. In all cases, genotyping was carried out with 24 microsatellite markers distributed in four multiplex panels with six markers each. Of the 392 F1 plants analyzed, obtained from 21 different biparental crosses, 325 (82.91%) was confirmed as real hybrids by the molecular analysis. The genetic analysis of the four BRS Estilo seed samples (two of certified seeds and two of saved seeds) detected 100.00% genetic identity with the samples used as control in only one certified seed sample.
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spelling Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common beanPhaseolus vulgarismolecular markersgenetic similaritymarker-assisted selectionplant breedingAbstract This manuscript reports on the application of microsatellite markers to assess the effectiveness of controlled crosses and assess the genetic identity of seed samples from a same common bean cultivar (BRS Estilo). The DNA was extracted from leaf tissue collected from F1 plants and their parents as well as from seed samples of the cv. BRS Estilo by the alkaline lysis method. In all cases, genotyping was carried out with 24 microsatellite markers distributed in four multiplex panels with six markers each. Of the 392 F1 plants analyzed, obtained from 21 different biparental crosses, 325 (82.91%) was confirmed as real hybrids by the molecular analysis. The genetic analysis of the four BRS Estilo seed samples (two of certified seeds and two of saved seeds) detected 100.00% genetic identity with the samples used as control in only one certified seed sample.Crop Breeding and Applied Biotechnology2016-09-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000300234Crop Breeding and Applied Biotechnology v.16 n.3 2016reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332016v16n3n35info:eu-repo/semantics/openAccessMorais,Samara Rayane Pereira deVieira,Ariadna FariaAlmeida,Laura Cristina da SilvaRodrigues,Luana AlvesMelo,Patrícia Guimarães SantosFaria,Luís Cláudio deMelo,Leonardo CunhaPereira,Helton SantosSouza,Thiago Lívio Pessoa Oliveira deeng2017-05-18T00:00:00Zoai:scielo:S1984-70332016000300234Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2017-05-18T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean
title Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean
spellingShingle Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean
Morais,Samara Rayane Pereira de
Phaseolus vulgaris
molecular markers
genetic similarity
marker-assisted selection
plant breeding
title_short Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean
title_full Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean
title_fullStr Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean
title_full_unstemmed Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean
title_sort Application of microsatellite markers to confirm controlled crosses and assess genetic identity in common bean
author Morais,Samara Rayane Pereira de
author_facet Morais,Samara Rayane Pereira de
Vieira,Ariadna Faria
Almeida,Laura Cristina da Silva
Rodrigues,Luana Alves
Melo,Patrícia Guimarães Santos
Faria,Luís Cláudio de
Melo,Leonardo Cunha
Pereira,Helton Santos
Souza,Thiago Lívio Pessoa Oliveira de
author_role author
author2 Vieira,Ariadna Faria
Almeida,Laura Cristina da Silva
Rodrigues,Luana Alves
Melo,Patrícia Guimarães Santos
Faria,Luís Cláudio de
Melo,Leonardo Cunha
Pereira,Helton Santos
Souza,Thiago Lívio Pessoa Oliveira de
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Morais,Samara Rayane Pereira de
Vieira,Ariadna Faria
Almeida,Laura Cristina da Silva
Rodrigues,Luana Alves
Melo,Patrícia Guimarães Santos
Faria,Luís Cláudio de
Melo,Leonardo Cunha
Pereira,Helton Santos
Souza,Thiago Lívio Pessoa Oliveira de
dc.subject.por.fl_str_mv Phaseolus vulgaris
molecular markers
genetic similarity
marker-assisted selection
plant breeding
topic Phaseolus vulgaris
molecular markers
genetic similarity
marker-assisted selection
plant breeding
description Abstract This manuscript reports on the application of microsatellite markers to assess the effectiveness of controlled crosses and assess the genetic identity of seed samples from a same common bean cultivar (BRS Estilo). The DNA was extracted from leaf tissue collected from F1 plants and their parents as well as from seed samples of the cv. BRS Estilo by the alkaline lysis method. In all cases, genotyping was carried out with 24 microsatellite markers distributed in four multiplex panels with six markers each. Of the 392 F1 plants analyzed, obtained from 21 different biparental crosses, 325 (82.91%) was confirmed as real hybrids by the molecular analysis. The genetic analysis of the four BRS Estilo seed samples (two of certified seeds and two of saved seeds) detected 100.00% genetic identity with the samples used as control in only one certified seed sample.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000300234
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000300234
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332016v16n3n35
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.16 n.3 2016
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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