Genetic variability among cassava accessions based on SSR markers

Detalhes bibliográficos
Autor(a) principal: Ribeiro,Márcia de Nazaré Oliveira
Data de Publicação: 2011
Outros Autores: Carvalho,Samuel Pereira de, Santos,João Bosco dos, Antonio,Rafaela Priscila
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332011000300009
Resumo: The aim of this study was to characterize and estimate the genetic similarity among 93 cassava accessions. The DNA amplification was performed with 14 microsatellite primers. The amplification products were separated by a polyacrylamide gel electrophoresis, showing a polymorphism formation, through which the accessions were discriminated against. The genetic similarity among accessions of cassava was estimated by the Dice coefficient. Cluster analysis was carried out using the UPGMA method. The polymorphic primers amplified a total of 26 alleles with 2-4 alleles per loci. The genetic similarity ranged from 0.16 to 0.96. The average values for observed and expected heterozygosity were 0.18 and 0.46, respectively. Twenty genetic similarity clusters were determined, demonstrating diversity among accessions, suggesting the possibility of heterotic hybrid generation.
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spelling Genetic variability among cassava accessions based on SSR markersManihot esculentamicrosatellitesgenetic characterizationThe aim of this study was to characterize and estimate the genetic similarity among 93 cassava accessions. The DNA amplification was performed with 14 microsatellite primers. The amplification products were separated by a polyacrylamide gel electrophoresis, showing a polymorphism formation, through which the accessions were discriminated against. The genetic similarity among accessions of cassava was estimated by the Dice coefficient. Cluster analysis was carried out using the UPGMA method. The polymorphic primers amplified a total of 26 alleles with 2-4 alleles per loci. The genetic similarity ranged from 0.16 to 0.96. The average values for observed and expected heterozygosity were 0.18 and 0.46, respectively. Twenty genetic similarity clusters were determined, demonstrating diversity among accessions, suggesting the possibility of heterotic hybrid generation.Crop Breeding and Applied Biotechnology2011-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332011000300009Crop Breeding and Applied Biotechnology v.11 n.3 2011reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/S1984-70332011000300009info:eu-repo/semantics/openAccessRibeiro,Márcia de Nazaré OliveiraCarvalho,Samuel Pereira deSantos,João Bosco dosAntonio,Rafaela Priscilaeng2011-10-17T00:00:00Zoai:scielo:S1984-70332011000300009Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2011-10-17T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Genetic variability among cassava accessions based on SSR markers
title Genetic variability among cassava accessions based on SSR markers
spellingShingle Genetic variability among cassava accessions based on SSR markers
Ribeiro,Márcia de Nazaré Oliveira
Manihot esculenta
microsatellites
genetic characterization
title_short Genetic variability among cassava accessions based on SSR markers
title_full Genetic variability among cassava accessions based on SSR markers
title_fullStr Genetic variability among cassava accessions based on SSR markers
title_full_unstemmed Genetic variability among cassava accessions based on SSR markers
title_sort Genetic variability among cassava accessions based on SSR markers
author Ribeiro,Márcia de Nazaré Oliveira
author_facet Ribeiro,Márcia de Nazaré Oliveira
Carvalho,Samuel Pereira de
Santos,João Bosco dos
Antonio,Rafaela Priscila
author_role author
author2 Carvalho,Samuel Pereira de
Santos,João Bosco dos
Antonio,Rafaela Priscila
author2_role author
author
author
dc.contributor.author.fl_str_mv Ribeiro,Márcia de Nazaré Oliveira
Carvalho,Samuel Pereira de
Santos,João Bosco dos
Antonio,Rafaela Priscila
dc.subject.por.fl_str_mv Manihot esculenta
microsatellites
genetic characterization
topic Manihot esculenta
microsatellites
genetic characterization
description The aim of this study was to characterize and estimate the genetic similarity among 93 cassava accessions. The DNA amplification was performed with 14 microsatellite primers. The amplification products were separated by a polyacrylamide gel electrophoresis, showing a polymorphism formation, through which the accessions were discriminated against. The genetic similarity among accessions of cassava was estimated by the Dice coefficient. Cluster analysis was carried out using the UPGMA method. The polymorphic primers amplified a total of 26 alleles with 2-4 alleles per loci. The genetic similarity ranged from 0.16 to 0.96. The average values for observed and expected heterozygosity were 0.18 and 0.46, respectively. Twenty genetic similarity clusters were determined, demonstrating diversity among accessions, suggesting the possibility of heterotic hybrid generation.
publishDate 2011
dc.date.none.fl_str_mv 2011-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332011000300009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332011000300009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1984-70332011000300009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.11 n.3 2011
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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