Genetic variability among cassava accessions based on SSR markers

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Márcia de Nazaré Oliveira
Data de Publicação: 2011
Outros Autores: Carvalho, Samuel Pereira de, Santos, João Bosco dos, Antonio, Rafaela Priscila
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/37863
Resumo: The aim of this study was to characterize and estimate the genetic similarity among 93 cassava accessions. The DNA amplification was performed with 14 microsatellite primers. The amplification products were separated by a polyacrylamide gel electrophoresis, showing a polymorphism formation, through which the accessions were discriminated against. The genetic similarity among accessions of cassava was estimated by the Dice coefficient. Cluster analysis was carried out using the UPGMA method. The polymorphic primers amplified a total of 26 alleles with 2-4 alleles per loci. The genetic similarity ranged from 0.16 to 0.96. The average values for observed and expected heterozygosity were 0.18 and 0.46, respectively. Twenty genetic similarity clusters were determined, demonstrating diversity among accessions, suggesting the possibility of heterotic hybrid generation.
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spelling Genetic variability among cassava accessions based on SSR markersVariabilidade genética entre acessos de mandioca com base em marcadores SSRManihot esculentaMicrossatélitesMicrosatellitesThe aim of this study was to characterize and estimate the genetic similarity among 93 cassava accessions. The DNA amplification was performed with 14 microsatellite primers. The amplification products were separated by a polyacrylamide gel electrophoresis, showing a polymorphism formation, through which the accessions were discriminated against. The genetic similarity among accessions of cassava was estimated by the Dice coefficient. Cluster analysis was carried out using the UPGMA method. The polymorphic primers amplified a total of 26 alleles with 2-4 alleles per loci. The genetic similarity ranged from 0.16 to 0.96. The average values for observed and expected heterozygosity were 0.18 and 0.46, respectively. Twenty genetic similarity clusters were determined, demonstrating diversity among accessions, suggesting the possibility of heterotic hybrid generation.Objetivou-se caracterizar e estimar a similaridade genética entre 93 acessos de mandioca. A amplificação do DNA foi realizada com 14 primers microssatélites. Os produtos de amplificação foram separados por eletroforese em gel de poliacrilamida, evidenciando-se a formação de bandas polimórficas, por meio das quais os acessos foram discriminados. A similaridade genética entre os acessos de mandioca foi estimada por meio do coeficiente de Dice. A análise de agrupamento foi feita com o método UPGMA. 26 alelos foram amplificados com 2 a 4 alelos por loco. O coeficiente de similaridade genética variou de 0,16 a 0,96. Os valores médios para heterozigosidade observada e esperada foram 0,18 e 0,46, respectivamente. 20 agrupamentos de similaridade genética foram detectados, demonstrando a existência de diversidade entre os acessos, sugerindo a possibilidade de geração de híbridos heteróticos.Crop Breeding and Applied Biotechnology2019-11-28T18:10:42Z2019-11-28T18:10:42Z2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfRIBEIRO, M. de N. O.; CARVALHO, S. P. de; SANTOS, J. B. dos; ANTONIO, R. P. Genetic variability among cassava accessions based on SSR markers. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 11, n. 3, p. 263-269, 2011.http://repositorio.ufla.br/jspui/handle/1/37863Crop Breeding and Applied Biotechnologyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessRibeiro, Márcia de Nazaré OliveiraCarvalho, Samuel Pereira deSantos, João Bosco dosAntonio, Rafaela Priscilaeng2019-11-28T18:10:42Zoai:localhost:1/37863Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-11-28T18:10:42Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Genetic variability among cassava accessions based on SSR markers
Variabilidade genética entre acessos de mandioca com base em marcadores SSR
title Genetic variability among cassava accessions based on SSR markers
spellingShingle Genetic variability among cassava accessions based on SSR markers
Ribeiro, Márcia de Nazaré Oliveira
Manihot esculenta
Microssatélites
Microsatellites
title_short Genetic variability among cassava accessions based on SSR markers
title_full Genetic variability among cassava accessions based on SSR markers
title_fullStr Genetic variability among cassava accessions based on SSR markers
title_full_unstemmed Genetic variability among cassava accessions based on SSR markers
title_sort Genetic variability among cassava accessions based on SSR markers
author Ribeiro, Márcia de Nazaré Oliveira
author_facet Ribeiro, Márcia de Nazaré Oliveira
Carvalho, Samuel Pereira de
Santos, João Bosco dos
Antonio, Rafaela Priscila
author_role author
author2 Carvalho, Samuel Pereira de
Santos, João Bosco dos
Antonio, Rafaela Priscila
author2_role author
author
author
dc.contributor.author.fl_str_mv Ribeiro, Márcia de Nazaré Oliveira
Carvalho, Samuel Pereira de
Santos, João Bosco dos
Antonio, Rafaela Priscila
dc.subject.por.fl_str_mv Manihot esculenta
Microssatélites
Microsatellites
topic Manihot esculenta
Microssatélites
Microsatellites
description The aim of this study was to characterize and estimate the genetic similarity among 93 cassava accessions. The DNA amplification was performed with 14 microsatellite primers. The amplification products were separated by a polyacrylamide gel electrophoresis, showing a polymorphism formation, through which the accessions were discriminated against. The genetic similarity among accessions of cassava was estimated by the Dice coefficient. Cluster analysis was carried out using the UPGMA method. The polymorphic primers amplified a total of 26 alleles with 2-4 alleles per loci. The genetic similarity ranged from 0.16 to 0.96. The average values for observed and expected heterozygosity were 0.18 and 0.46, respectively. Twenty genetic similarity clusters were determined, demonstrating diversity among accessions, suggesting the possibility of heterotic hybrid generation.
publishDate 2011
dc.date.none.fl_str_mv 2011
2019-11-28T18:10:42Z
2019-11-28T18:10:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv RIBEIRO, M. de N. O.; CARVALHO, S. P. de; SANTOS, J. B. dos; ANTONIO, R. P. Genetic variability among cassava accessions based on SSR markers. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 11, n. 3, p. 263-269, 2011.
http://repositorio.ufla.br/jspui/handle/1/37863
identifier_str_mv RIBEIRO, M. de N. O.; CARVALHO, S. P. de; SANTOS, J. B. dos; ANTONIO, R. P. Genetic variability among cassava accessions based on SSR markers. Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 11, n. 3, p. 263-269, 2011.
url http://repositorio.ufla.br/jspui/handle/1/37863
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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