Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum

Detalhes bibliográficos
Autor(a) principal: Antiqueira,Lia Maris Orth Ritter
Data de Publicação: 2016
Outros Autores: Dequigiovanni,Gabriel, Tambarussi,Evandro Vagner, Rodrigues,Jucelene Fernandes, Veasey,Elizabeth Ann
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Crop Breeding and Applied Biotechnology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000400355
Resumo: Abstract Studies of genetic diversity and structure are key elements in designing effective in situ and ex situ management plans, especially for species experiencing forest fragmentation. To investigate the level of genetic diversity in populations of Trichocentrum pumilum, eight polymorphic microsatellite loci were developed and used for genotyping 96 specimens from four disturbed populations. Low genetic diversity within populations was found (average number of alleles per locus ranging from 3.75 to 4.25, observed and expected heterozygosities from 0.238 to 0.333 and from 0.450 to 0.482, respectively). The fixation index () ranged from 0.35 to 0.47, with significant values for all populations. No genotypic disequilibrium was detected. A mixed breeding system was found through an apparent outcrossing rate estimate. Our results suggest that these microsatellite loci are suitable for genetic studies of this species, showing low within population genetic diversity and moderate structure for T. pumilum populations.
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spelling Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilumConservation geneticsgenetic diversityOrchidaceaepopulation geneticstropical orchid speciesAbstract Studies of genetic diversity and structure are key elements in designing effective in situ and ex situ management plans, especially for species experiencing forest fragmentation. To investigate the level of genetic diversity in populations of Trichocentrum pumilum, eight polymorphic microsatellite loci were developed and used for genotyping 96 specimens from four disturbed populations. Low genetic diversity within populations was found (average number of alleles per locus ranging from 3.75 to 4.25, observed and expected heterozygosities from 0.238 to 0.333 and from 0.450 to 0.482, respectively). The fixation index () ranged from 0.35 to 0.47, with significant values for all populations. No genotypic disequilibrium was detected. A mixed breeding system was found through an apparent outcrossing rate estimate. Our results suggest that these microsatellite loci are suitable for genetic studies of this species, showing low within population genetic diversity and moderate structure for T. pumilum populations.Crop Breeding and Applied Biotechnology2016-12-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000400355Crop Breeding and Applied Biotechnology v.16 n.4 2016reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332016v16n4n52info:eu-repo/semantics/openAccessAntiqueira,Lia Maris Orth RitterDequigiovanni,GabrielTambarussi,Evandro VagnerRodrigues,Jucelene FernandesVeasey,Elizabeth Anneng2016-12-19T00:00:00Zoai:scielo:S1984-70332016000400355Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2016-12-19T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse
dc.title.none.fl_str_mv Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum
title Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum
spellingShingle Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum
Antiqueira,Lia Maris Orth Ritter
Conservation genetics
genetic diversity
Orchidaceae
population genetics
tropical orchid species
title_short Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum
title_full Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum
title_fullStr Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum
title_full_unstemmed Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum
title_sort Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum
author Antiqueira,Lia Maris Orth Ritter
author_facet Antiqueira,Lia Maris Orth Ritter
Dequigiovanni,Gabriel
Tambarussi,Evandro Vagner
Rodrigues,Jucelene Fernandes
Veasey,Elizabeth Ann
author_role author
author2 Dequigiovanni,Gabriel
Tambarussi,Evandro Vagner
Rodrigues,Jucelene Fernandes
Veasey,Elizabeth Ann
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Antiqueira,Lia Maris Orth Ritter
Dequigiovanni,Gabriel
Tambarussi,Evandro Vagner
Rodrigues,Jucelene Fernandes
Veasey,Elizabeth Ann
dc.subject.por.fl_str_mv Conservation genetics
genetic diversity
Orchidaceae
population genetics
tropical orchid species
topic Conservation genetics
genetic diversity
Orchidaceae
population genetics
tropical orchid species
description Abstract Studies of genetic diversity and structure are key elements in designing effective in situ and ex situ management plans, especially for species experiencing forest fragmentation. To investigate the level of genetic diversity in populations of Trichocentrum pumilum, eight polymorphic microsatellite loci were developed and used for genotyping 96 specimens from four disturbed populations. Low genetic diversity within populations was found (average number of alleles per locus ranging from 3.75 to 4.25, observed and expected heterozygosities from 0.238 to 0.333 and from 0.450 to 0.482, respectively). The fixation index () ranged from 0.35 to 0.47, with significant values for all populations. No genotypic disequilibrium was detected. A mixed breeding system was found through an apparent outcrossing rate estimate. Our results suggest that these microsatellite loci are suitable for genetic studies of this species, showing low within population genetic diversity and moderate structure for T. pumilum populations.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000400355
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000400355
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1984-70332016v16n4n52
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
publisher.none.fl_str_mv Crop Breeding and Applied Biotechnology
dc.source.none.fl_str_mv Crop Breeding and Applied Biotechnology v.16 n.4 2016
reponame:Crop Breeding and Applied Biotechnology
instname:Sociedade Brasileira de Melhoramento de Plantas
instacron:CBAB
instname_str Sociedade Brasileira de Melhoramento de Plantas
instacron_str CBAB
institution CBAB
reponame_str Crop Breeding and Applied Biotechnology
collection Crop Breeding and Applied Biotechnology
repository.name.fl_str_mv Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas
repository.mail.fl_str_mv cbabjournal@gmail.com||cbab@ufv.br
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