Vertical transmission of toxocara canis in successive generations of mice

Detalhes bibliográficos
Autor(a) principal: Schoenardie, Elizandra Roselaine
Data de Publicação: 2013
Outros Autores: Scaini, Carlos James, Pepe, Michele Soares, Borsuk, Sibele, Avila, Luciana Farias da Costa de, Villela, Marcos Marreiro, Berne, Maria Elisabeth Aires
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FURG (RI FURG)
Texto Completo: http://repositorio.furg.br/handle/1/7064
Resumo: Visceral toxocariasis is a neglected zoonosis caused by Toxocara canis larvae in unusual hosts. In dogs, the definitive host, the infection occurs mainly through transplacental and transcolostral transmission. Studies on experimental models have shown that vertical transmission may result from acute infections. Considering that toxocariasis is characterized as a chronic infection, with possible reactivation of larvae present in the brain, this study evaluated the presence of larvae in the brain of female BALB/c mice and their offspring with chronic infection during three successive pregnancies. ELISA-TES was used to evaluate the antibody levels. T. canis larvae were detected in the brain tissue of the mice during the three successive generations evaluated. The offspring’s IgG level gradually decreased, and mean absorbance (ABS) above the cutoff point (0.070) was observed only at 30 (0.229) and 50 (0.096) days of age, while IgM was not detected. The infections in the offspring confirmed that vertical transmission of T. canis larvae occurred during chronic toxocariasis in three successive generations of mice.
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spelling Schoenardie, Elizandra RoselaineScaini, Carlos JamesPepe, Michele SoaresBorsuk, SibeleAvila, Luciana Farias da Costa deVillela, Marcos MarreiroBerne, Maria Elisabeth Aires2017-02-09T18:15:06Z2017-02-09T18:15:06Z2013SCHOENARDIE, Elizandra Roselaine et al. Vertical transmission of toxocara canis in successive generations of mice. Revista Brasileira de Parasitologia Veterinária, v. 22, n.4, p. 623-626, 2013. Disponível em: <http://www.scielo.br/pdf/rbpv/v22n4/0103-846x-rbpv-22-04-623.pdf>. Acesso em: 04 fev. 2017.1984-2961http://repositorio.furg.br/handle/1/706410.1590/S1984-29612013000400030Visceral toxocariasis is a neglected zoonosis caused by Toxocara canis larvae in unusual hosts. In dogs, the definitive host, the infection occurs mainly through transplacental and transcolostral transmission. Studies on experimental models have shown that vertical transmission may result from acute infections. Considering that toxocariasis is characterized as a chronic infection, with possible reactivation of larvae present in the brain, this study evaluated the presence of larvae in the brain of female BALB/c mice and their offspring with chronic infection during three successive pregnancies. ELISA-TES was used to evaluate the antibody levels. T. canis larvae were detected in the brain tissue of the mice during the three successive generations evaluated. The offspring’s IgG level gradually decreased, and mean absorbance (ABS) above the cutoff point (0.070) was observed only at 30 (0.229) and 50 (0.096) days of age, while IgM was not detected. The infections in the offspring confirmed that vertical transmission of T. canis larvae occurred during chronic toxocariasis in three successive generations of mice.A toxocaríase visceral é uma zoonose negligenciada causada por larvas de Toxocara canis em hospedeiros não usuais. Em cães, os hospedeiros definitivos, a infecção ocorre normalmente por transmissão transplacentária e através do colostro. Estudos com modelos experimentais têm demonstrado a ocorrência de transmissão vertical durante a infecção aguda. Considerando que a toxocaríase é caracterizada como uma infecção crônica, com uma possível reativação das larvas presentes no cérebro, este estudo avaliou a presença de larvas no cérebro de camundongos Balb/C fêmeas e suas proles com infecção crônica durante três gestações sucessivas. Para avaliar os níveis de anticorpos foi utilizado ELISA-TES. Larvas de T. canis foram detectadas no encéfalo dos animais durante as três gerações sucessivas avaliadas. O nível de IgG das proles foi diminuindo gradualmente e as médias de absorbâncias (ABS) acima do ponto de corte (0,070) foram evidenciadas somente aos 30 (0,229) e 50 dias (0,096) de vida, enquanto que não foi detectada IgM. Infecções das proles confirmam a transmissão vertical de larvas de T. canis durante a toxocaríase crônica em três gerações sucessivas de camundongos.engVisceral larva migransToxocara canisVertical transmissionBALB/c miceELISA - TESLarva migrans visceralTransmissão verticalCamundongos BALB/cVertical transmission of toxocara canis in successive generations of miceTransmissão vertical de toxocara canis em gerações sucessivas de camundongosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGORIGINAL0103-846x-rbpv-22-04-623.pdf0103-846x-rbpv-22-04-623.pdfapplication/pdf697735https://repositorio.furg.br/bitstream/1/7064/1/0103-846x-rbpv-22-04-623.pdf6b4aa60ff8efea64793de8eeccb0a1e5MD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.furg.br/bitstream/1/7064/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52open access1/70642022-10-19 11:25:40.113open accessoai:repositorio.furg.br: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Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2022-10-19T14:25:40Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false
dc.title.pt_BR.fl_str_mv Vertical transmission of toxocara canis in successive generations of mice
dc.title.alternative.pt_BR.fl_str_mv Transmissão vertical de toxocara canis em gerações sucessivas de camundongos
title Vertical transmission of toxocara canis in successive generations of mice
spellingShingle Vertical transmission of toxocara canis in successive generations of mice
Schoenardie, Elizandra Roselaine
Visceral larva migrans
Toxocara canis
Vertical transmission
BALB/c mice
ELISA - TES
Larva migrans visceral
Transmissão vertical
Camundongos BALB/c
title_short Vertical transmission of toxocara canis in successive generations of mice
title_full Vertical transmission of toxocara canis in successive generations of mice
title_fullStr Vertical transmission of toxocara canis in successive generations of mice
title_full_unstemmed Vertical transmission of toxocara canis in successive generations of mice
title_sort Vertical transmission of toxocara canis in successive generations of mice
author Schoenardie, Elizandra Roselaine
author_facet Schoenardie, Elizandra Roselaine
Scaini, Carlos James
Pepe, Michele Soares
Borsuk, Sibele
Avila, Luciana Farias da Costa de
Villela, Marcos Marreiro
Berne, Maria Elisabeth Aires
author_role author
author2 Scaini, Carlos James
Pepe, Michele Soares
Borsuk, Sibele
Avila, Luciana Farias da Costa de
Villela, Marcos Marreiro
Berne, Maria Elisabeth Aires
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Schoenardie, Elizandra Roselaine
Scaini, Carlos James
Pepe, Michele Soares
Borsuk, Sibele
Avila, Luciana Farias da Costa de
Villela, Marcos Marreiro
Berne, Maria Elisabeth Aires
dc.subject.por.fl_str_mv Visceral larva migrans
Toxocara canis
Vertical transmission
BALB/c mice
ELISA - TES
Larva migrans visceral
Transmissão vertical
Camundongos BALB/c
topic Visceral larva migrans
Toxocara canis
Vertical transmission
BALB/c mice
ELISA - TES
Larva migrans visceral
Transmissão vertical
Camundongos BALB/c
description Visceral toxocariasis is a neglected zoonosis caused by Toxocara canis larvae in unusual hosts. In dogs, the definitive host, the infection occurs mainly through transplacental and transcolostral transmission. Studies on experimental models have shown that vertical transmission may result from acute infections. Considering that toxocariasis is characterized as a chronic infection, with possible reactivation of larvae present in the brain, this study evaluated the presence of larvae in the brain of female BALB/c mice and their offspring with chronic infection during three successive pregnancies. ELISA-TES was used to evaluate the antibody levels. T. canis larvae were detected in the brain tissue of the mice during the three successive generations evaluated. The offspring’s IgG level gradually decreased, and mean absorbance (ABS) above the cutoff point (0.070) was observed only at 30 (0.229) and 50 (0.096) days of age, while IgM was not detected. The infections in the offspring confirmed that vertical transmission of T. canis larvae occurred during chronic toxocariasis in three successive generations of mice.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2017-02-09T18:15:06Z
dc.date.available.fl_str_mv 2017-02-09T18:15:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv SCHOENARDIE, Elizandra Roselaine et al. Vertical transmission of toxocara canis in successive generations of mice. Revista Brasileira de Parasitologia Veterinária, v. 22, n.4, p. 623-626, 2013. Disponível em: <http://www.scielo.br/pdf/rbpv/v22n4/0103-846x-rbpv-22-04-623.pdf>. Acesso em: 04 fev. 2017.
dc.identifier.uri.fl_str_mv http://repositorio.furg.br/handle/1/7064
dc.identifier.issn.none.fl_str_mv 1984-2961
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/S1984-29612013000400030
identifier_str_mv SCHOENARDIE, Elizandra Roselaine et al. Vertical transmission of toxocara canis in successive generations of mice. Revista Brasileira de Parasitologia Veterinária, v. 22, n.4, p. 623-626, 2013. Disponível em: <http://www.scielo.br/pdf/rbpv/v22n4/0103-846x-rbpv-22-04-623.pdf>. Acesso em: 04 fev. 2017.
1984-2961
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