O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição Genotípica

Detalhes bibliográficos
Autor(a) principal: Viana, Aline Cavalcanti
Data de Publicação: 2009
Outros Autores: Curtis, Karen Maria de Carvalho, Kim, Yeon Jung, Orrico, Silvana Regina Perez, Cano, Veridiana S.P., Valentini, Sandro Roberto, Scarel-Caminaga, Raquel Mantuaneli
Tipo de documento: Artigo
Idioma: por
Título da fonte: Saúde e Pesquisa (Online)
Texto Completo: https://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/793
Resumo: The single nucleotide polymorphism (SNP) +781(C/T) (rs2227306) in the interleukin 8 gene (IL8) has been largely investigated in case-control association studies. Different methods have been used to genotype individuals for this SNP, but they demand extensive optimization of polymerase chain reaction (PCR) conditions or high cost reagents and equipment. The aim of this study was to develop a simple and efficient assay for the detection of the polymorphism rs2227306 in the IL8 gene and to execute an interethnic comparison of the genotype distribution in different populations. The Amplification-Created Restriction Site (ACRS) method was used for genotyping 174 healthy Brazilian individuals (Whites n=148; Blacks n=26). This method was efficient, reproducible and accurate, since the genotypes were confirmed by sequencing. White Brazilian subgroup showed a genotype distribution similar to HapMap CEU Europeans (p=0.49), but different from a German population, PGA African American and HapMap Sub-Saharan Africans (p
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spelling O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição GenotípicaAmplification-Created Restriction Site (Acrs) Method for the Detection of the Polymorphism Rs2227306 in the Interleukin 8 Gene and Interethnic Comparison of Genotype DistributionACRSEthnicInterleukin-8MethodPolymorphismrs2227306EtniaInterleucina-8PolimorfismoThe single nucleotide polymorphism (SNP) +781(C/T) (rs2227306) in the interleukin 8 gene (IL8) has been largely investigated in case-control association studies. Different methods have been used to genotype individuals for this SNP, but they demand extensive optimization of polymerase chain reaction (PCR) conditions or high cost reagents and equipment. The aim of this study was to develop a simple and efficient assay for the detection of the polymorphism rs2227306 in the IL8 gene and to execute an interethnic comparison of the genotype distribution in different populations. The Amplification-Created Restriction Site (ACRS) method was used for genotyping 174 healthy Brazilian individuals (Whites n=148; Blacks n=26). This method was efficient, reproducible and accurate, since the genotypes were confirmed by sequencing. White Brazilian subgroup showed a genotype distribution similar to HapMap CEU Europeans (p=0.49), but different from a German population, PGA African American and HapMap Sub-Saharan Africans (pO polimorfismo de base única (SNP - single nucleotide polymorphism) +781(C/T) (rs2227306) no gene interleucina (IL8) tem sido largamente investigado em estudos de associação caso-controle. Diferentes métodos têm sido utilizados para genotipar indivíduos para este SNP, mas eles demandam extensiva otimização das condições de PCR (Polymerase Chain Reaction) ou alto custo de reagentes e equipamentos. O objetivo deste estudo foi desenvolver um método simples e eficiente para detectar o polimorfismo rs2227306 no gene IL8 e também realizar a comparação interétnica da distribuição genotípica em diferentes populações. O método ACRS (The Amplification-Created Restriction Site) foi usado para a genotipagem de 174 indivíduos brasileiros saudáveis (brancos n=148; negros n=26). Observou-se que o presente método foi eficiente, reprodutível e acurado, tendo sido os genótipos confirmados por sequenciamento. O subgrupo de brasileiros brancos mostrou uma distribuição genotípica similar ao HapMap CEU europeus (p=0,49), mas foi diferente da população alemã, PGA afro-americano e HapMap africanos subsaarianos (pUniversidade Cesumar - UniCesumar2009-06-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por ParesPesquisa Originalapplication/pdfhttps://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/793Saúde e Pesquisa; Vol 2 No 1 (2009): jan./abr.; 27-31Saúde e Pesquisa; v. 2 n. 1 (2009): jan./abr.; 27-312176-9206reponame:Saúde e Pesquisa (Online)instname:Cesumar Diretoria de Pesquisainstacron:CESUMARporhttps://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/793/719Viana, Aline CavalcantiCurtis, Karen Maria de CarvalhoKim, Yeon JungOrrico, Silvana Regina PerezCano, Veridiana S.P.Valentini, Sandro RobertoScarel-Caminaga, Raquel Mantuaneliinfo:eu-repo/semantics/openAccess2022-05-23T19:12:36Zoai:ojs.pkp.sfu.ca:article/793Revistahttps://periodicos.unicesumar.edu.br/index.php/saudpesqPUBhttps://periodicos.unicesumar.edu.br/index.php/saudpesq/oainaep@cesumar.br2176-92061983-1870opendoar:2022-05-23T19:12:36Saúde e Pesquisa (Online) - Cesumar Diretoria de Pesquisafalse
dc.title.none.fl_str_mv O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição Genotípica
Amplification-Created Restriction Site (Acrs) Method for the Detection of the Polymorphism Rs2227306 in the Interleukin 8 Gene and Interethnic Comparison of Genotype Distribution
title O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição Genotípica
spellingShingle O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição Genotípica
Viana, Aline Cavalcanti
ACRS
Ethnic
Interleukin-8
Method
Polymorphism
rs2227306
Etnia
Interleucina-8
Polimorfismo
title_short O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição Genotípica
title_full O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição Genotípica
title_fullStr O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição Genotípica
title_full_unstemmed O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição Genotípica
title_sort O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição Genotípica
author Viana, Aline Cavalcanti
author_facet Viana, Aline Cavalcanti
Curtis, Karen Maria de Carvalho
Kim, Yeon Jung
Orrico, Silvana Regina Perez
Cano, Veridiana S.P.
Valentini, Sandro Roberto
Scarel-Caminaga, Raquel Mantuaneli
author_role author
author2 Curtis, Karen Maria de Carvalho
Kim, Yeon Jung
Orrico, Silvana Regina Perez
Cano, Veridiana S.P.
Valentini, Sandro Roberto
Scarel-Caminaga, Raquel Mantuaneli
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Viana, Aline Cavalcanti
Curtis, Karen Maria de Carvalho
Kim, Yeon Jung
Orrico, Silvana Regina Perez
Cano, Veridiana S.P.
Valentini, Sandro Roberto
Scarel-Caminaga, Raquel Mantuaneli
dc.subject.por.fl_str_mv ACRS
Ethnic
Interleukin-8
Method
Polymorphism
rs2227306
Etnia
Interleucina-8
Polimorfismo
topic ACRS
Ethnic
Interleukin-8
Method
Polymorphism
rs2227306
Etnia
Interleucina-8
Polimorfismo
description The single nucleotide polymorphism (SNP) +781(C/T) (rs2227306) in the interleukin 8 gene (IL8) has been largely investigated in case-control association studies. Different methods have been used to genotype individuals for this SNP, but they demand extensive optimization of polymerase chain reaction (PCR) conditions or high cost reagents and equipment. The aim of this study was to develop a simple and efficient assay for the detection of the polymorphism rs2227306 in the IL8 gene and to execute an interethnic comparison of the genotype distribution in different populations. The Amplification-Created Restriction Site (ACRS) method was used for genotyping 174 healthy Brazilian individuals (Whites n=148; Blacks n=26). This method was efficient, reproducible and accurate, since the genotypes were confirmed by sequencing. White Brazilian subgroup showed a genotype distribution similar to HapMap CEU Europeans (p=0.49), but different from a German population, PGA African American and HapMap Sub-Saharan Africans (p
publishDate 2009
dc.date.none.fl_str_mv 2009-06-08
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por Pares
Pesquisa Original
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/793
url https://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/793
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/793/719
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Cesumar - UniCesumar
publisher.none.fl_str_mv Universidade Cesumar - UniCesumar
dc.source.none.fl_str_mv Saúde e Pesquisa; Vol 2 No 1 (2009): jan./abr.; 27-31
Saúde e Pesquisa; v. 2 n. 1 (2009): jan./abr.; 27-31
2176-9206
reponame:Saúde e Pesquisa (Online)
instname:Cesumar Diretoria de Pesquisa
instacron:CESUMAR
instname_str Cesumar Diretoria de Pesquisa
instacron_str CESUMAR
institution CESUMAR
reponame_str Saúde e Pesquisa (Online)
collection Saúde e Pesquisa (Online)
repository.name.fl_str_mv Saúde e Pesquisa (Online) - Cesumar Diretoria de Pesquisa
repository.mail.fl_str_mv naep@cesumar.br
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