O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição Genotípica
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Saúde e Pesquisa (Online) |
Texto Completo: | https://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/793 |
Resumo: | The single nucleotide polymorphism (SNP) +781(C/T) (rs2227306) in the interleukin 8 gene (IL8) has been largely investigated in case-control association studies. Different methods have been used to genotype individuals for this SNP, but they demand extensive optimization of polymerase chain reaction (PCR) conditions or high cost reagents and equipment. The aim of this study was to develop a simple and efficient assay for the detection of the polymorphism rs2227306 in the IL8 gene and to execute an interethnic comparison of the genotype distribution in different populations. The Amplification-Created Restriction Site (ACRS) method was used for genotyping 174 healthy Brazilian individuals (Whites n=148; Blacks n=26). This method was efficient, reproducible and accurate, since the genotypes were confirmed by sequencing. White Brazilian subgroup showed a genotype distribution similar to HapMap CEU Europeans (p=0.49), but different from a German population, PGA African American and HapMap Sub-Saharan Africans (p |
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O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição GenotípicaAmplification-Created Restriction Site (Acrs) Method for the Detection of the Polymorphism Rs2227306 in the Interleukin 8 Gene and Interethnic Comparison of Genotype DistributionACRSEthnicInterleukin-8MethodPolymorphismrs2227306EtniaInterleucina-8PolimorfismoThe single nucleotide polymorphism (SNP) +781(C/T) (rs2227306) in the interleukin 8 gene (IL8) has been largely investigated in case-control association studies. Different methods have been used to genotype individuals for this SNP, but they demand extensive optimization of polymerase chain reaction (PCR) conditions or high cost reagents and equipment. The aim of this study was to develop a simple and efficient assay for the detection of the polymorphism rs2227306 in the IL8 gene and to execute an interethnic comparison of the genotype distribution in different populations. The Amplification-Created Restriction Site (ACRS) method was used for genotyping 174 healthy Brazilian individuals (Whites n=148; Blacks n=26). This method was efficient, reproducible and accurate, since the genotypes were confirmed by sequencing. White Brazilian subgroup showed a genotype distribution similar to HapMap CEU Europeans (p=0.49), but different from a German population, PGA African American and HapMap Sub-Saharan Africans (pO polimorfismo de base única (SNP - single nucleotide polymorphism) +781(C/T) (rs2227306) no gene interleucina (IL8) tem sido largamente investigado em estudos de associação caso-controle. Diferentes métodos têm sido utilizados para genotipar indivíduos para este SNP, mas eles demandam extensiva otimização das condições de PCR (Polymerase Chain Reaction) ou alto custo de reagentes e equipamentos. O objetivo deste estudo foi desenvolver um método simples e eficiente para detectar o polimorfismo rs2227306 no gene IL8 e também realizar a comparação interétnica da distribuição genotípica em diferentes populações. O método ACRS (The Amplification-Created Restriction Site) foi usado para a genotipagem de 174 indivíduos brasileiros saudáveis (brancos n=148; negros n=26). Observou-se que o presente método foi eficiente, reprodutível e acurado, tendo sido os genótipos confirmados por sequenciamento. O subgrupo de brasileiros brancos mostrou uma distribuição genotípica similar ao HapMap CEU europeus (p=0,49), mas foi diferente da população alemã, PGA afro-americano e HapMap africanos subsaarianos (pUniversidade Cesumar - UniCesumar2009-06-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por ParesPesquisa Originalapplication/pdfhttps://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/793Saúde e Pesquisa; Vol 2 No 1 (2009): jan./abr.; 27-31Saúde e Pesquisa; v. 2 n. 1 (2009): jan./abr.; 27-312176-9206reponame:Saúde e Pesquisa (Online)instname:Cesumar Diretoria de Pesquisainstacron:CESUMARporhttps://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/793/719Viana, Aline CavalcantiCurtis, Karen Maria de CarvalhoKim, Yeon JungOrrico, Silvana Regina PerezCano, Veridiana S.P.Valentini, Sandro RobertoScarel-Caminaga, Raquel Mantuaneliinfo:eu-repo/semantics/openAccess2022-05-23T19:12:36Zoai:ojs.pkp.sfu.ca:article/793Revistahttps://periodicos.unicesumar.edu.br/index.php/saudpesqPUBhttps://periodicos.unicesumar.edu.br/index.php/saudpesq/oainaep@cesumar.br2176-92061983-1870opendoar:2022-05-23T19:12:36Saúde e Pesquisa (Online) - Cesumar Diretoria de Pesquisafalse |
dc.title.none.fl_str_mv |
O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição Genotípica Amplification-Created Restriction Site (Acrs) Method for the Detection of the Polymorphism Rs2227306 in the Interleukin 8 Gene and Interethnic Comparison of Genotype Distribution |
title |
O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição Genotípica |
spellingShingle |
O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição Genotípica Viana, Aline Cavalcanti ACRS Ethnic Interleukin-8 Method Polymorphism rs2227306 Etnia Interleucina-8 Polimorfismo |
title_short |
O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição Genotípica |
title_full |
O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição Genotípica |
title_fullStr |
O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição Genotípica |
title_full_unstemmed |
O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição Genotípica |
title_sort |
O Método ACRS (Amplification-Created Restriction Site) na Detecção do Polimorfismo rs2227306 no Gene Interleucina (Il8) e Comparação Interétnica da Distribuição Genotípica |
author |
Viana, Aline Cavalcanti |
author_facet |
Viana, Aline Cavalcanti Curtis, Karen Maria de Carvalho Kim, Yeon Jung Orrico, Silvana Regina Perez Cano, Veridiana S.P. Valentini, Sandro Roberto Scarel-Caminaga, Raquel Mantuaneli |
author_role |
author |
author2 |
Curtis, Karen Maria de Carvalho Kim, Yeon Jung Orrico, Silvana Regina Perez Cano, Veridiana S.P. Valentini, Sandro Roberto Scarel-Caminaga, Raquel Mantuaneli |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Viana, Aline Cavalcanti Curtis, Karen Maria de Carvalho Kim, Yeon Jung Orrico, Silvana Regina Perez Cano, Veridiana S.P. Valentini, Sandro Roberto Scarel-Caminaga, Raquel Mantuaneli |
dc.subject.por.fl_str_mv |
ACRS Ethnic Interleukin-8 Method Polymorphism rs2227306 Etnia Interleucina-8 Polimorfismo |
topic |
ACRS Ethnic Interleukin-8 Method Polymorphism rs2227306 Etnia Interleucina-8 Polimorfismo |
description |
The single nucleotide polymorphism (SNP) +781(C/T) (rs2227306) in the interleukin 8 gene (IL8) has been largely investigated in case-control association studies. Different methods have been used to genotype individuals for this SNP, but they demand extensive optimization of polymerase chain reaction (PCR) conditions or high cost reagents and equipment. The aim of this study was to develop a simple and efficient assay for the detection of the polymorphism rs2227306 in the IL8 gene and to execute an interethnic comparison of the genotype distribution in different populations. The Amplification-Created Restriction Site (ACRS) method was used for genotyping 174 healthy Brazilian individuals (Whites n=148; Blacks n=26). This method was efficient, reproducible and accurate, since the genotypes were confirmed by sequencing. White Brazilian subgroup showed a genotype distribution similar to HapMap CEU Europeans (p=0.49), but different from a German population, PGA African American and HapMap Sub-Saharan Africans (p |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-06-08 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por Pares Pesquisa Original |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/793 |
url |
https://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/793 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.unicesumar.edu.br/index.php/saudpesq/article/view/793/719 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Cesumar - UniCesumar |
publisher.none.fl_str_mv |
Universidade Cesumar - UniCesumar |
dc.source.none.fl_str_mv |
Saúde e Pesquisa; Vol 2 No 1 (2009): jan./abr.; 27-31 Saúde e Pesquisa; v. 2 n. 1 (2009): jan./abr.; 27-31 2176-9206 reponame:Saúde e Pesquisa (Online) instname:Cesumar Diretoria de Pesquisa instacron:CESUMAR |
instname_str |
Cesumar Diretoria de Pesquisa |
instacron_str |
CESUMAR |
institution |
CESUMAR |
reponame_str |
Saúde e Pesquisa (Online) |
collection |
Saúde e Pesquisa (Online) |
repository.name.fl_str_mv |
Saúde e Pesquisa (Online) - Cesumar Diretoria de Pesquisa |
repository.mail.fl_str_mv |
naep@cesumar.br |
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1754122531480010752 |