Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation
| Autor(a) principal: | |
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| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/53245 |
Resumo: | Universidade Federal de São Paulo. Departamento de Química. CDMF, LIEC. São Carlos, SP, Brasil / Department of Physical and Analytical Chemistry, University Jaume I (UJI), 12071 Castelló, Spain. |
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Pereira, Paula Fabiana SantosSilva, Ana Carolina Alves de Paula ePimentel, Bruna Natália Alves da SilvaPinatti, Ivo MateusSimões, Alexandre ZirpoliVergani, Carlos EduardoVieira, Débora Ferreira BarretoSilva, Marcos Alexandre Nunes daMiranda, Milene DiasMonteiro, Maria Eduarda SantosTucci, AmandaDoñate‑Buendía, CarlosMínguez‑Vega, GladysAndrés, JuanLongo, Elson2022-06-14T12:43:30Z2022-06-14T12:43:30Z2022PEREIRA, Paula Fabiana Santos et al. Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation. Scientific Reports, v. 12, 8118, p. 1-18, 2022.2045-2322https://www.arca.fiocruz.br/handle/icict/5324510.1038/s41598-022-11902-5engNatureSARS‑CoV‑2COVID-19Inativação do SARS-CoV-2Composto quitosana/α-Ag2WO4Gerado por laserPandemiaSARS‑CoV‑2COVID-19Inactivation of SARS‑CoV‑2Chitosan/α‑Ag2WO4 compositeSecond laser irradiationPandemicInactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Paulo. Departamento de Química. CDMF, LIEC. São Carlos, SP, Brasil / Department of Physical and Analytical Chemistry, University Jaume I (UJI), 12071 Castelló, Spain.Universidade Estadual de São Paulo. Escola de Odontologia. Departamento de Materiais Dentários e Prótese Dentária. São Paulo, SP, Brasil.Universidade Estadual de São Paulo. Escola de Odontologia. Departamento de Materiais Dentários e Prótese Dentária. São Paulo, SP, Brasil.Department of Physical and Analytical Chemistry, University Jaume I (UJI), 12071 Castelló, Spain / Universidade Estadual de São Paulo. Faculdade de Engenharia de Guaratinguetá, SP, Brasil.Universidade Estadual de São Paulo. Faculdade de Engenharia de Guaratinguetá, SP, Brasil.Universidade Estadual de São Paulo. Escola de Odontologia. Departamento de Materiais Dentários e Prótese Dentária. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.GROC UJI, Institute of New Imaging Technologies, Universitat Jaume I, Avda. Sos Baynat sn, 12071 Castellón de la Plana, Spain / Materials Science and Additive Manufacturing, University of Wuppertal, Gaußstr. 20, 42119 Wuppertal, Germany.GROC UJI, Institute of New Imaging Technologies, Universitat Jaume I, Avda. Sos Baynat sn, 12071 Castellón de la Plana, Spain.Department of Physical and Analytical Chemistry, University Jaume I (UJI), 12071 Castelló, Spain.Universidade Federal de São Paulo. Departamento de Química. CDMF, LIEC. São Carlos, SP, Brasil.In the current COVID-19 pandemic, the next generation of innovative materials with enhanced anti-SARS-CoV-2 activity is urgently needed to prevent the spread of this virus within the community. Herein, we report the synthesis of chitosan/α-Ag2WO4 composites synthetized by femtosecond laser irradiation. The antimicrobial activity against Escherichia coli, Methicilin-susceptible Staphylococcus aureus (MSSA), and Candida albicans was determined by estimating the minimum inhibitory concentration (MIC) and minimal bactericidal/fungicidal concentration (MBC/MFC). To assess the biocompatibility of chitosan/α-Ag2WO4 composites in a range involving MIC and MBC/MFC on keratinocytes cells (NOK-si), an alamarBlue™ assay and an MTT assay were carried out. The SARSCoV- 2 virucidal effects was analyzed in Vero E6 cells through viral titer quantified in cell culture supernatant by PFU/mL assay. Our results showed a very similar antimicrobial activity of chitosan/α- Ag2WO4 3.3 and 6.6, with the last one demonstrating a slightly better action against MSSA. The chitosan/α-Ag2WO4 9.9 showed a wide range of antimicrobial activity (0.49–31.25 μg/mL). The cytotoxicity outcomes by alamarBlue™ revealed that the concentrations of interest (MIC and MBC/ MFC) were considered non-cytotoxic to all composites after 72 h of exposure. The Chitosan/α-Ag2WO4 (CS6.6/α-Ag2WO4) composite reduced the SARS-CoV-2 viral titer quantification up to 80% of the controls. Then, our results suggest that these composites are highly efficient materials to kill bacteria (Escherichia coli, Methicillin-susceptible Staphylococcus aureus, and the yeast strain Candida albicans), in addition to inactivating SARS-CoV-2 by contact, through ROS production.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/53245/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMarcosANSilva_MileneDMiranda_etal_IOC_2022.pdfMarcosANSilva_MileneDMiranda_etal_IOC_2022.pdfapplication/pdf4444402https://www.arca.fiocruz.br/bitstream/icict/53245/2/MarcosANSilva_MileneDMiranda_etal_IOC_2022.pdf292cb6301aa4945e0c3f4b6447528a18MD52icict/532452023-02-15 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| dc.title.pt_BR.fl_str_mv |
Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation |
| title |
Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation |
| spellingShingle |
Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation Pereira, Paula Fabiana Santos SARS‑CoV‑2 COVID-19 Inativação do SARS-CoV-2 Composto quitosana/α-Ag2WO4 Gerado por laser Pandemia SARS‑CoV‑2 COVID-19 Inactivation of SARS‑CoV‑2 Chitosan/α‑Ag2WO4 composite Second laser irradiation Pandemic |
| title_short |
Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation |
| title_full |
Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation |
| title_fullStr |
Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation |
| title_full_unstemmed |
Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation |
| title_sort |
Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation |
| author |
Pereira, Paula Fabiana Santos |
| author_facet |
Pereira, Paula Fabiana Santos Silva, Ana Carolina Alves de Paula e Pimentel, Bruna Natália Alves da Silva Pinatti, Ivo Mateus Simões, Alexandre Zirpoli Vergani, Carlos Eduardo Vieira, Débora Ferreira Barreto Silva, Marcos Alexandre Nunes da Miranda, Milene Dias Monteiro, Maria Eduarda Santos Tucci, Amanda Doñate‑Buendía, Carlos Mínguez‑Vega, Gladys Andrés, Juan Longo, Elson |
| author_role |
author |
| author2 |
Silva, Ana Carolina Alves de Paula e Pimentel, Bruna Natália Alves da Silva Pinatti, Ivo Mateus Simões, Alexandre Zirpoli Vergani, Carlos Eduardo Vieira, Débora Ferreira Barreto Silva, Marcos Alexandre Nunes da Miranda, Milene Dias Monteiro, Maria Eduarda Santos Tucci, Amanda Doñate‑Buendía, Carlos Mínguez‑Vega, Gladys Andrés, Juan Longo, Elson |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Pereira, Paula Fabiana Santos Silva, Ana Carolina Alves de Paula e Pimentel, Bruna Natália Alves da Silva Pinatti, Ivo Mateus Simões, Alexandre Zirpoli Vergani, Carlos Eduardo Vieira, Débora Ferreira Barreto Silva, Marcos Alexandre Nunes da Miranda, Milene Dias Monteiro, Maria Eduarda Santos Tucci, Amanda Doñate‑Buendía, Carlos Mínguez‑Vega, Gladys Andrés, Juan Longo, Elson |
| dc.subject.other.pt_BR.fl_str_mv |
SARS‑CoV‑2 COVID-19 Inativação do SARS-CoV-2 Composto quitosana/α-Ag2WO4 Gerado por laser |
| topic |
SARS‑CoV‑2 COVID-19 Inativação do SARS-CoV-2 Composto quitosana/α-Ag2WO4 Gerado por laser Pandemia SARS‑CoV‑2 COVID-19 Inactivation of SARS‑CoV‑2 Chitosan/α‑Ag2WO4 composite Second laser irradiation Pandemic |
| dc.subject.other.none.fl_str_mv |
Pandemia |
| dc.subject.en.pt_BR.fl_str_mv |
SARS‑CoV‑2 COVID-19 Inactivation of SARS‑CoV‑2 Chitosan/α‑Ag2WO4 composite Second laser irradiation |
| dc.subject.en.none.fl_str_mv |
Pandemic |
| description |
Universidade Federal de São Paulo. Departamento de Química. CDMF, LIEC. São Carlos, SP, Brasil / Department of Physical and Analytical Chemistry, University Jaume I (UJI), 12071 Castelló, Spain. |
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2022 |
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2022-06-14T12:43:30Z |
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2022-06-14T12:43:30Z |
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2022 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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PEREIRA, Paula Fabiana Santos et al. Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation. Scientific Reports, v. 12, 8118, p. 1-18, 2022. |
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https://www.arca.fiocruz.br/handle/icict/53245 |
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2045-2322 |
| dc.identifier.doi.none.fl_str_mv |
10.1038/s41598-022-11902-5 |
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PEREIRA, Paula Fabiana Santos et al. Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation. Scientific Reports, v. 12, 8118, p. 1-18, 2022. 2045-2322 10.1038/s41598-022-11902-5 |
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Nature |
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