Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation

Detalhes bibliográficos
Autor(a) principal: Pereira, Paula Fabiana Santos
Data de Publicação: 2022
Outros Autores: Silva, Ana Carolina Alves de Paula e, Pimentel, Bruna Natália Alves da Silva, Pinatti, Ivo Mateus, Simões, Alexandre Zirpoli, Vergani, Carlos Eduardo, Vieira, Débora Ferreira Barreto, Silva, Marcos Alexandre Nunes da, Miranda, Milene Dias, Monteiro, Maria Eduarda Santos, Tucci, Amanda, Doñate‑Buendía, Carlos, Mínguez‑Vega, Gladys, Andrés, Juan, Longo, Elson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/53245
Resumo: Universidade Federal de São Paulo. Departamento de Química. CDMF, LIEC. São Carlos, SP, Brasil / Department of Physical and Analytical Chemistry, University Jaume I (UJI), 12071 Castelló, Spain.
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spelling Pereira, Paula Fabiana SantosSilva, Ana Carolina Alves de Paula ePimentel, Bruna Natália Alves da SilvaPinatti, Ivo MateusSimões, Alexandre ZirpoliVergani, Carlos EduardoVieira, Débora Ferreira BarretoSilva, Marcos Alexandre Nunes daMiranda, Milene DiasMonteiro, Maria Eduarda SantosTucci, AmandaDoñate‑Buendía, CarlosMínguez‑Vega, GladysAndrés, JuanLongo, Elson2022-06-14T12:43:30Z2022-06-14T12:43:30Z2022PEREIRA, Paula Fabiana Santos et al. Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation. Scientific Reports, v. 12, 8118, p. 1-18, 2022.2045-2322https://www.arca.fiocruz.br/handle/icict/5324510.1038/s41598-022-11902-5engNatureSARS‑CoV‑2COVID-19Inativação do SARS-CoV-2Composto quitosana/α-Ag2WO4Gerado por laserPandemiaSARS‑CoV‑2COVID-19Inactivation of SARS‑CoV‑2Chitosan/α‑Ag2WO4 compositeSecond laser irradiationPandemicInactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Paulo. Departamento de Química. CDMF, LIEC. São Carlos, SP, Brasil / Department of Physical and Analytical Chemistry, University Jaume I (UJI), 12071 Castelló, Spain.Universidade Estadual de São Paulo. Escola de Odontologia. Departamento de Materiais Dentários e Prótese Dentária. São Paulo, SP, Brasil.Universidade Estadual de São Paulo. Escola de Odontologia. Departamento de Materiais Dentários e Prótese Dentária. São Paulo, SP, Brasil.Department of Physical and Analytical Chemistry, University Jaume I (UJI), 12071 Castelló, Spain / Universidade Estadual de São Paulo. Faculdade de Engenharia de Guaratinguetá, SP, Brasil.Universidade Estadual de São Paulo. Faculdade de Engenharia de Guaratinguetá, SP, Brasil.Universidade Estadual de São Paulo. Escola de Odontologia. Departamento de Materiais Dentários e Prótese Dentária. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.GROC UJI, Institute of New Imaging Technologies, Universitat Jaume I, Avda. Sos Baynat sn, 12071 Castellón de la Plana, Spain / Materials Science and Additive Manufacturing, University of Wuppertal, Gaußstr. 20, 42119 Wuppertal, Germany.GROC UJI, Institute of New Imaging Technologies, Universitat Jaume I, Avda. Sos Baynat sn, 12071 Castellón de la Plana, Spain.Department of Physical and Analytical Chemistry, University Jaume I (UJI), 12071 Castelló, Spain.Universidade Federal de São Paulo. Departamento de Química. CDMF, LIEC. São Carlos, SP, Brasil.In the current COVID-19 pandemic, the next generation of innovative materials with enhanced anti-SARS-CoV-2 activity is urgently needed to prevent the spread of this virus within the community. Herein, we report the synthesis of chitosan/α-Ag2WO4 composites synthetized by femtosecond laser irradiation. The antimicrobial activity against Escherichia coli, Methicilin-susceptible Staphylococcus aureus (MSSA), and Candida albicans was determined by estimating the minimum inhibitory concentration (MIC) and minimal bactericidal/fungicidal concentration (MBC/MFC). To assess the biocompatibility of chitosan/α-Ag2WO4 composites in a range involving MIC and MBC/MFC on keratinocytes cells (NOK-si), an alamarBlue™ assay and an MTT assay were carried out. The SARSCoV- 2 virucidal effects was analyzed in Vero E6 cells through viral titer quantified in cell culture supernatant by PFU/mL assay. Our results showed a very similar antimicrobial activity of chitosan/α- Ag2WO4 3.3 and 6.6, with the last one demonstrating a slightly better action against MSSA. The chitosan/α-Ag2WO4 9.9 showed a wide range of antimicrobial activity (0.49–31.25 μg/mL). The cytotoxicity outcomes by alamarBlue™ revealed that the concentrations of interest (MIC and MBC/ MFC) were considered non-cytotoxic to all composites after 72 h of exposure. The Chitosan/α-Ag2WO4 (CS6.6/α-Ag2WO4) composite reduced the SARS-CoV-2 viral titer quantification up to 80% of the controls. Then, our results suggest that these composites are highly efficient materials to kill bacteria (Escherichia coli, Methicillin-susceptible Staphylococcus aureus, and the yeast strain Candida albicans), in addition to inactivating SARS-CoV-2 by contact, through ROS production.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/53245/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMarcosANSilva_MileneDMiranda_etal_IOC_2022.pdfMarcosANSilva_MileneDMiranda_etal_IOC_2022.pdfapplication/pdf4444402https://www.arca.fiocruz.br/bitstream/icict/53245/2/MarcosANSilva_MileneDMiranda_etal_IOC_2022.pdf292cb6301aa4945e0c3f4b6447528a18MD52icict/532452023-02-15 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dc.title.pt_BR.fl_str_mv Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation
title Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation
spellingShingle Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation
Pereira, Paula Fabiana Santos
SARS‑CoV‑2
COVID-19
Inativação do SARS-CoV-2
Composto quitosana/α-Ag2WO4
Gerado por laser
Pandemia
SARS‑CoV‑2
COVID-19
Inactivation of SARS‑CoV‑2
Chitosan/α‑Ag2WO4 composite
Second laser irradiation
Pandemic
title_short Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation
title_full Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation
title_fullStr Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation
title_full_unstemmed Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation
title_sort Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation
author Pereira, Paula Fabiana Santos
author_facet Pereira, Paula Fabiana Santos
Silva, Ana Carolina Alves de Paula e
Pimentel, Bruna Natália Alves da Silva
Pinatti, Ivo Mateus
Simões, Alexandre Zirpoli
Vergani, Carlos Eduardo
Vieira, Débora Ferreira Barreto
Silva, Marcos Alexandre Nunes da
Miranda, Milene Dias
Monteiro, Maria Eduarda Santos
Tucci, Amanda
Doñate‑Buendía, Carlos
Mínguez‑Vega, Gladys
Andrés, Juan
Longo, Elson
author_role author
author2 Silva, Ana Carolina Alves de Paula e
Pimentel, Bruna Natália Alves da Silva
Pinatti, Ivo Mateus
Simões, Alexandre Zirpoli
Vergani, Carlos Eduardo
Vieira, Débora Ferreira Barreto
Silva, Marcos Alexandre Nunes da
Miranda, Milene Dias
Monteiro, Maria Eduarda Santos
Tucci, Amanda
Doñate‑Buendía, Carlos
Mínguez‑Vega, Gladys
Andrés, Juan
Longo, Elson
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira, Paula Fabiana Santos
Silva, Ana Carolina Alves de Paula e
Pimentel, Bruna Natália Alves da Silva
Pinatti, Ivo Mateus
Simões, Alexandre Zirpoli
Vergani, Carlos Eduardo
Vieira, Débora Ferreira Barreto
Silva, Marcos Alexandre Nunes da
Miranda, Milene Dias
Monteiro, Maria Eduarda Santos
Tucci, Amanda
Doñate‑Buendía, Carlos
Mínguez‑Vega, Gladys
Andrés, Juan
Longo, Elson
dc.subject.other.pt_BR.fl_str_mv SARS‑CoV‑2
COVID-19
Inativação do SARS-CoV-2
Composto quitosana/α-Ag2WO4
Gerado por laser
topic SARS‑CoV‑2
COVID-19
Inativação do SARS-CoV-2
Composto quitosana/α-Ag2WO4
Gerado por laser
Pandemia
SARS‑CoV‑2
COVID-19
Inactivation of SARS‑CoV‑2
Chitosan/α‑Ag2WO4 composite
Second laser irradiation
Pandemic
dc.subject.other.none.fl_str_mv Pandemia
dc.subject.en.pt_BR.fl_str_mv SARS‑CoV‑2
COVID-19
Inactivation of SARS‑CoV‑2
Chitosan/α‑Ag2WO4 composite
Second laser irradiation
dc.subject.en.none.fl_str_mv Pandemic
description Universidade Federal de São Paulo. Departamento de Química. CDMF, LIEC. São Carlos, SP, Brasil / Department of Physical and Analytical Chemistry, University Jaume I (UJI), 12071 Castelló, Spain.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-06-14T12:43:30Z
dc.date.available.fl_str_mv 2022-06-14T12:43:30Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PEREIRA, Paula Fabiana Santos et al. Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation. Scientific Reports, v. 12, 8118, p. 1-18, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/53245
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-022-11902-5
identifier_str_mv PEREIRA, Paula Fabiana Santos et al. Inactivation of SARS‑CoV‑2 by a chitosan/α‑Ag2WO4 composite generated by femtosecond laser irradiation. Scientific Reports, v. 12, 8118, p. 1-18, 2022.
2045-2322
10.1038/s41598-022-11902-5
url https://www.arca.fiocruz.br/handle/icict/53245
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Nature
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