Molecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazil

Detalhes bibliográficos
Autor(a) principal: Galhardo, Maria Clara Gutierrez
Data de Publicação: 2008
Outros Autores: Oliveira, Rosely Maria Zancopé de, Valle, Antonio Carlos Francesconi do, Paes, Rodrigo de Almeida, Tavares, Patricia Morais e Silva, Monzon, Araceli, Mellado, Emilia, Rodriguez-Tudela, Juan L., Cuenca-Estrella, Manuel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33263
Resumo: 2020-05-29
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spelling Galhardo, Maria Clara GutierrezOliveira, Rosely Maria Zancopé deValle, Antonio Carlos Francesconi doPaes, Rodrigo de AlmeidaTavares, Patricia Morais e SilvaMonzon, AraceliMellado, EmiliaRodriguez-Tudela, Juan L.Cuenca-Estrella, Manuel2019-05-29T13:05:21Z2019-05-29T13:05:21Z2008GALHARDO, Maria Clara Gutierrez et al. Molecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazil. Medical Mycology, v. 46, p. 141-151, 2008.1369-3786https://www.arca.fiocruz.br/handle/icict/3326310.1080/136937807017423991460-2709engOxford University PressMolecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-05-29Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Infectologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Infectologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ, Brasil.Instituto de Salud Carlos III. Centro Nacional de Microbiología. Servicio de Micología. Madrid, Spain.Instituto de Salud Carlos III. Centro Nacional de Microbiología. Servicio de Micología. Madrid, Spain.Instituto de Salud Carlos III. Centro Nacional de Microbiología. Servicio de Micología. Madrid, Spain.Instituto de Salud Carlos III. Centro Nacional de Microbiología. Servicio de Micología. Madrid, Spain.Since 1998 a cat-transmitted epidemic of sporotrichosis has been observed in Rio de Janeiro (RJ), Brazil. Besides the lymphocutaneous and fixed forms, other presentations, such as disseminated cutaneous and mucosal involvement, as well as for the first time, erythema nodosum and erythema multiforme have been reported associated with sporotrichosis. This study investigates the phenotypes and genotypes of Sporothrix schenckii isolates recovered from different clinical forms of the disease noted as part of this epidemic. A total of 88 isolates recovered from 59 cases associated with the epidemic and 29 controls (from cases in other Brazilian regions and Spain) were included in this study. In vitro susceptibility testing was conducted as part of the phenotypic analysis, while the genotypic analysis involved a DNA fingerprinting method with primer M13 and ribosomal DNA sequencing of the internal transcribed spacer (ITS). MIC values of amphotericin B, itraconazole, posaconazole, ravuconazole, and terbinafine were found to be significantly lower (P<0.01) for isolates associated with the epidemic than for control strains. No differences in MIC values were observed related to clinical forms of the infection. Fingerprinting analysis showed that RJ epidemic strains were genetically related. Although nine subtypes were found, they were not associated with specific clinical forms. Similar results were obtained with the ITS sequence analysis. These data suggest that the strains isolated from the epidemic cases of sporotrichosis in RJ all originated from a common source.Sporothrix schenckiiIn vitro susceptibilityPCR fingerprintingClinical formsITS regioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/33263/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_Galhardo_Maria_etal_INI_2008.pdfve_Galhardo_Maria_etal_INI_2008.pdfapplication/pdf451886https://www.arca.fiocruz.br/bitstream/icict/33263/2/ve_Galhardo_Maria_etal_INI_2008.pdfbddeb15f69fc4e1601b2b07990025bcdMD52TEXTve_Galhardo_Maria_etal_INI_2008.pdf.txtve_Galhardo_Maria_etal_INI_2008.pdf.txtExtracted texttext/plain44246https://www.arca.fiocruz.br/bitstream/icict/33263/3/ve_Galhardo_Maria_etal_INI_2008.pdf.txt7edbd85894049ec58a3fe1e1673f68ecMD53icict/332632021-03-24 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dc.title.pt_BR.fl_str_mv Molecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazil
title Molecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazil
spellingShingle Molecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazil
Galhardo, Maria Clara Gutierrez
Sporothrix schenckii
In vitro susceptibility
PCR fingerprinting
Clinical forms
ITS region
title_short Molecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazil
title_full Molecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazil
title_fullStr Molecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazil
title_full_unstemmed Molecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazil
title_sort Molecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazil
author Galhardo, Maria Clara Gutierrez
author_facet Galhardo, Maria Clara Gutierrez
Oliveira, Rosely Maria Zancopé de
Valle, Antonio Carlos Francesconi do
Paes, Rodrigo de Almeida
Tavares, Patricia Morais e Silva
Monzon, Araceli
Mellado, Emilia
Rodriguez-Tudela, Juan L.
Cuenca-Estrella, Manuel
author_role author
author2 Oliveira, Rosely Maria Zancopé de
Valle, Antonio Carlos Francesconi do
Paes, Rodrigo de Almeida
Tavares, Patricia Morais e Silva
Monzon, Araceli
Mellado, Emilia
Rodriguez-Tudela, Juan L.
Cuenca-Estrella, Manuel
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Galhardo, Maria Clara Gutierrez
Oliveira, Rosely Maria Zancopé de
Valle, Antonio Carlos Francesconi do
Paes, Rodrigo de Almeida
Tavares, Patricia Morais e Silva
Monzon, Araceli
Mellado, Emilia
Rodriguez-Tudela, Juan L.
Cuenca-Estrella, Manuel
dc.subject.en.pt_BR.fl_str_mv Sporothrix schenckii
In vitro susceptibility
PCR fingerprinting
Clinical forms
ITS region
topic Sporothrix schenckii
In vitro susceptibility
PCR fingerprinting
Clinical forms
ITS region
description 2020-05-29
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2019-05-29T13:05:21Z
dc.date.available.fl_str_mv 2019-05-29T13:05:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GALHARDO, Maria Clara Gutierrez et al. Molecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazil. Medical Mycology, v. 46, p. 141-151, 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33263
dc.identifier.issn.pt_BR.fl_str_mv 1369-3786
dc.identifier.doi.none.fl_str_mv 10.1080/13693780701742399
dc.identifier.eissn.none.fl_str_mv 1460-2709
identifier_str_mv GALHARDO, Maria Clara Gutierrez et al. Molecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazil. Medical Mycology, v. 46, p. 141-151, 2008.
1369-3786
10.1080/13693780701742399
1460-2709
url https://www.arca.fiocruz.br/handle/icict/33263
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/33263/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/33263/2/ve_Galhardo_Maria_etal_INI_2008.pdf
https://www.arca.fiocruz.br/bitstream/icict/33263/3/ve_Galhardo_Maria_etal_INI_2008.pdf.txt
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