Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/39899 |
Resumo: | São Paulo Research Foundation (FAPESP), process #2017/10906-8 |
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Costa, Sidnei FerroGomes, Vinícius OliveiraMaciel, Marilene Oliveira dos SantosMelo, Larissa MartinsVenturin, Gabriela LovizuttoBragato, Jaqueline PoletoRebech, Gabriela TorresSantos, Catiule de OliveiraOliveira, Bárbara Maria Nascimento deOliveira, Geraldo Gileno de SáLima, Valéria Marçal Felix de2020-02-12T12:40:38Z2020-02-12T12:40:38Z2020COSTA, Sidnei Ferro et al. Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis. PLoS Neglected Tropical Diseases, p. 1-21, 21 Jan. 2020.1935-2727https://www.arca.fiocruz.br/handle/icict/3989910.1371/journal.pntd.00080211935-2735São Paulo Research Foundation (FAPESP), process #2017/10906-8National Council for Scientific and Technological Development (CNPq), process #400063/2016-6CNPq/INCT-DT, process #573839/2008-5CNPq-PROEP, process #400913/2013-5Coordination for the Improvement of Higher Education Personnel (CAPES) - Finance Code 001Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil.Domestic dogs are the main reservoir of Leishmania infantum, a causative agent of visceral leishmaniasis (VL). The number of human disease cases is associated with the rate of canine infection. Currently available drugs are not efficient at treating canine leishmaniasis (CanL) and months after the treatment most dogs show disease relapse, therefore the development of new drugs or new therapeutic strategies should be sought. In CanL, dogs lack the ability to mount a specific cellular immune response suitable for combating the parasite and manipulation of cytokine signaling pathway has the potential to form part of effective immunotherapeutic methods. In this study, recombinant canine cytokines (rcaIL-12, rcaIL-2, rcaIL-15 and rcaIL-7) and soluble receptor IL-10R1 (rcasIL-10R1), with antagonistic activity, were evaluated for the first time in combination (rcaIL-12/rcaIL-2, rcaIL-12/rcaIL-15, rcaIL-12/rcasIL-10R1, rcaIL-15/rcaIL-7) or alone (rcasIL-10R1) to evaluate their immunomodulatory capacity in peripheral blood mononuclear cells (PBMC) from dogs with leishmaniasis. All the combinations of recombinant proteins tested were shown to improve lymphoproliferative response. Further, the combinations rcaIL-12/rcaIL-2 and rcaIL- 12/rcaIL-15 promoted a decrease in programmed cell death protein 1 (PD-1) expression in lymphocytes. These same combinations of cytokines and rcaIL-12/rcasIL-10R1 induced IFN-γ and TNF-α production in PBMC. Furthermore, the combination IL-12/IL-15 led to an increased in T-bet expression in lymphocytes. These findings are encouraging and indicate the use of rcaIL-12 and rcaIL-15 in future in vivo studies aimed at achieving polarization of cellular immune responses in dogs with leishmaniasis, which may contribute to the development of an effective treatment against CanL.engPublic Library of Sciencehttps://www.arca.fiocruz.br/handle/icict/40815Leishmaniose visceralIL-12IL-15Leishmania infantumImunidade celularVisceral leishmaniasisIl-12IL-15Leishmania infantumImmunity CellularCombined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNãoPublicadoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/39899/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCosta, S.F. 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| dc.title.pt_BR.fl_str_mv |
Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis |
| title |
Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis |
| spellingShingle |
Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis Costa, Sidnei Ferro Leishmaniose visceral IL-12 IL-15 Leishmania infantum Imunidade celular Visceral leishmaniasis Il-12 IL-15 Leishmania infantum Immunity Cellular |
| title_short |
Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis |
| title_full |
Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis |
| title_fullStr |
Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis |
| title_full_unstemmed |
Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis |
| title_sort |
Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis |
| author |
Costa, Sidnei Ferro |
| author_facet |
Costa, Sidnei Ferro Gomes, Vinícius Oliveira Maciel, Marilene Oliveira dos Santos Melo, Larissa Martins Venturin, Gabriela Lovizutto Bragato, Jaqueline Poleto Rebech, Gabriela Torres Santos, Catiule de Oliveira Oliveira, Bárbara Maria Nascimento de Oliveira, Geraldo Gileno de Sá Lima, Valéria Marçal Felix de |
| author_role |
author |
| author2 |
Gomes, Vinícius Oliveira Maciel, Marilene Oliveira dos Santos Melo, Larissa Martins Venturin, Gabriela Lovizutto Bragato, Jaqueline Poleto Rebech, Gabriela Torres Santos, Catiule de Oliveira Oliveira, Bárbara Maria Nascimento de Oliveira, Geraldo Gileno de Sá Lima, Valéria Marçal Felix de |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Costa, Sidnei Ferro Gomes, Vinícius Oliveira Maciel, Marilene Oliveira dos Santos Melo, Larissa Martins Venturin, Gabriela Lovizutto Bragato, Jaqueline Poleto Rebech, Gabriela Torres Santos, Catiule de Oliveira Oliveira, Bárbara Maria Nascimento de Oliveira, Geraldo Gileno de Sá Lima, Valéria Marçal Felix de |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmaniose visceral IL-12 IL-15 Leishmania infantum Imunidade celular |
| topic |
Leishmaniose visceral IL-12 IL-15 Leishmania infantum Imunidade celular Visceral leishmaniasis Il-12 IL-15 Leishmania infantum Immunity Cellular |
| dc.subject.en.pt_BR.fl_str_mv |
Visceral leishmaniasis Il-12 IL-15 Leishmania infantum Immunity Cellular |
| description |
São Paulo Research Foundation (FAPESP), process #2017/10906-8 |
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2020 |
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2020-02-12T12:40:38Z |
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2020-02-12T12:40:38Z |
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2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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COSTA, Sidnei Ferro et al. Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis. PLoS Neglected Tropical Diseases, p. 1-21, 21 Jan. 2020. |
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https://www.arca.fiocruz.br/handle/icict/39899 |
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1935-2727 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1371/journal.pntd.0008021 |
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1935-2735 |
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COSTA, Sidnei Ferro et al. Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis. PLoS Neglected Tropical Diseases, p. 1-21, 21 Jan. 2020. 1935-2727 10.1371/journal.pntd.0008021 1935-2735 |
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