Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/14187 |
Resumo: | p. 1-11 |
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Costa, Dirceu JoaquimCarvalho, Rayssa M. de AraujoAbbehusen, Melissa Moura CostaTeixeira, Clarissa RomeroPitombo, Maiana AlbuquerqueSantos, Joelma Trigo dosNascimento, Flávia Raquel Fernandes doSilva, Lucilene AmorimSilva, Ana Lucia AbreuCruz, Maria do Socorro PiresMiranda, José CarlosFukutani, Kyoshi FerreiraOliveira, Camila Indiani deBarral, Aldina Maria PradoBarral-Netto, ManoelBrodskyn, Claudia Ida2013-12-16T14:10:09Z2013-12-16T14:10:09Z20131932-6203http://repositorio.ufba.br/ri/handle/ri/14187v. 8, n. 4p. 1-11Background: Canine Visceral Leishmaniasis (CVL) is a zoonotic disease caused by Leishmania infantum, transmitted by the bite of Lutzomyia longipalpis sand flies. Dogs are the main domestic reservoir of the parasite. The establishment of an experimental model that partially reproduces natural infection in dogs is very important to test vaccine candidates, mainly regarding those that use salivary proteins from the vector and new therapeutical approaches. Methodology/Principal Findings: In this report, we describe an experimental infection in dogs, using intradermal injection of Leishmania infantum plus salivary gland homogenate (SGH) of Lutzomyia longipalpis. Thirty-five dogs were infected with 16107 parasites combined with five pairs of Lutzomyia longipalpis salivary glands and followed for 450 days after infection and clinical, immunological and parasitological parameters were evaluated. Two hundred and ten days after infection we observed that 31,4% of dogs did not display detectable levels of anti-Leishmania antibodies but all presented different numbers of parasites in the lymph nodes. Animals with a positive xenodiagnosis had at least 3,356105 parasites in their lymph nodes. An increase of IFN-c and IL-10 levels was detected during infection. Twenty two percent of dogs developed symptoms of CVL during infection. Conclusion: The infection model described here shows some degree of similarity when compared with naturally infected dogs opening new perspectives for the study of CVL using an experimental model that employs the combination of parasites and sand fly saliva both present during natural transmission.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-11-26T20:07:11Z No. of bitstreams: 1 333333.pdf: 1604899 bytes, checksum: a78c2e529c64a3400dcb92cae6906a2a (MD5)Approved for entry into archive by Patricia Barroso (pbarroso@ufba.br) on 2013-12-16T14:10:09Z (GMT) No. of bitstreams: 1 333333.pdf: 1604899 bytes, checksum: a78c2e529c64a3400dcb92cae6906a2a (MD5)Made available in DSpace on 2013-12-16T14:10:09Z (GMT). No. of bitstreams: 1 333333.pdf: 1604899 bytes, checksum: a78c2e529c64a3400dcb92cae6906a2a (MD5) Previous issue date: 2013SalvadorPLoS ONEhttp://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0060535&representation=PDFreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBALeishmaniasis, VisceralLeishmania infantumLeishmaniasis VaccinesExperimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral LeishmaniasisPLoS ONEinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessengORIGINAL333333.pdf333333.pdfapplication/pdf1604899https://repositorio.ufba.br/bitstream/ri/14187/1/333333.pdfa78c2e529c64a3400dcb92cae6906a2aMD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/14187/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXT333333.pdf.txt333333.pdf.txtExtracted texttext/plain49619https://repositorio.ufba.br/bitstream/ri/14187/3/333333.pdf.txt2864d9b1dd8999cf7b485bddd56c3c7dMD53ri/141872022-02-21 00:10:17.283oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-02-21T03:10:17Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis |
| dc.title.alternative.pt_BR.fl_str_mv |
PLoS ONE |
| title |
Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis |
| spellingShingle |
Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis Costa, Dirceu Joaquim Leishmaniasis, Visceral Leishmania infantum Leishmaniasis Vaccines |
| title_short |
Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis |
| title_full |
Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis |
| title_fullStr |
Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis |
| title_full_unstemmed |
Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis |
| title_sort |
Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis |
| author |
Costa, Dirceu Joaquim |
| author_facet |
Costa, Dirceu Joaquim Carvalho, Rayssa M. de Araujo Abbehusen, Melissa Moura Costa Teixeira, Clarissa Romero Pitombo, Maiana Albuquerque Santos, Joelma Trigo dos Nascimento, Flávia Raquel Fernandes do Silva, Lucilene Amorim Silva, Ana Lucia Abreu Cruz, Maria do Socorro Pires Miranda, José Carlos Fukutani, Kyoshi Ferreira Oliveira, Camila Indiani de Barral, Aldina Maria Prado Barral-Netto, Manoel Brodskyn, Claudia Ida |
| author_role |
author |
| author2 |
Carvalho, Rayssa M. de Araujo Abbehusen, Melissa Moura Costa Teixeira, Clarissa Romero Pitombo, Maiana Albuquerque Santos, Joelma Trigo dos Nascimento, Flávia Raquel Fernandes do Silva, Lucilene Amorim Silva, Ana Lucia Abreu Cruz, Maria do Socorro Pires Miranda, José Carlos Fukutani, Kyoshi Ferreira Oliveira, Camila Indiani de Barral, Aldina Maria Prado Barral-Netto, Manoel Brodskyn, Claudia Ida |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Costa, Dirceu Joaquim Carvalho, Rayssa M. de Araujo Abbehusen, Melissa Moura Costa Teixeira, Clarissa Romero Pitombo, Maiana Albuquerque Santos, Joelma Trigo dos Nascimento, Flávia Raquel Fernandes do Silva, Lucilene Amorim Silva, Ana Lucia Abreu Cruz, Maria do Socorro Pires Miranda, José Carlos Fukutani, Kyoshi Ferreira Oliveira, Camila Indiani de Barral, Aldina Maria Prado Barral-Netto, Manoel Brodskyn, Claudia Ida |
| dc.subject.por.fl_str_mv |
Leishmaniasis, Visceral Leishmania infantum Leishmaniasis Vaccines |
| topic |
Leishmaniasis, Visceral Leishmania infantum Leishmaniasis Vaccines |
| description |
p. 1-11 |
| publishDate |
2013 |
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2013-12-16T14:10:09Z |
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2013-12-16T14:10:09Z |
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2013 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://repositorio.ufba.br/ri/handle/ri/14187 |
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1932-6203 |
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v. 8, n. 4 |
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1932-6203 v. 8, n. 4 |
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http://repositorio.ufba.br/ri/handle/ri/14187 |
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openAccess |
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PLoS ONE |
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PLoS ONE |
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