Platelet proteome reveals novel pathways of platelet activation and platelet-mediated immunoregulation in dengue

Detalhes bibliográficos
Autor(a) principal: Trugilho, Monique Ramos de Oliveira
Data de Publicação: 2017
Outros Autores: Hottz, Eugenio Damaceno, Brunoro, Giselle Villa Flor, Teixeira-Ferreira, André, Carvalho, Paulo Costa, Salazar, Gustavo Adolfo, Zimmerman, Guy A., Bozza, Fernando A., Bozza, Patrícia Torres, Perales, Jonas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20082
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.
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spelling Trugilho, Monique Ramos de OliveiraHottz, Eugenio DamacenoBrunoro, Giselle Villa FlorTeixeira-Ferreira, AndréCarvalho, Paulo CostaSalazar, Gustavo AdolfoZimmerman, Guy A.Bozza, Fernando A.Bozza, Patrícia TorresPerales, Jonas2017-07-13T17:33:00Z2017-07-13T17:33:00Z2017TRUGILHO, Monique Ramos de Oliveira et al. Platelet proteome reveals novel pathways of platelet activation and platelet-mediated immunoregulation in dengue. PLoS Pathogens, v. 13, n. 5, p. 1-27, May 2017.1553-7366https://www.arca.fiocruz.br/handle/icict/2008210.1371/journal.ppat.1006385engPublic Library of ScienceDengueAtivação plaquetáriaImmunoregulationProteomaDenguePlatelet proteomeImmunoregulationPlatelet activationPlatelet proteome reveals novel pathways of platelet activation and platelet-mediated immunoregulation in dengueinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil / Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Laboratório de Análise de Glicoconjugados. Juiz de Fora, MG, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, BrasilUniversity of Cape Town. Faculty of Health Sciences. Computational Biology Group. Cape Town, South Africa.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil / University of Utah. Department of Internal Medicine. Salt Lake City, UT, USA.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil.Dengue is the most prevalent human arbovirus disease worldwide. Dengue virus (DENV) infection causes syndromes varying from self-limiting febrile illness to severe dengue. Although dengue pathophysiology is not completely understood, it is widely accepted that increased inflammation plays important roles in dengue pathogenesis. Platelets are blood cells classically known as effectors of hemostasis which have been increasingly recognized to have major immune and inflammatory activities. Nevertheless, the phenotype and effector functions of platelets in dengue pathogenesis are not completely understood. Here we used quantitative proteomics to investigate the protein content of platelets in clinical samples from patients with dengue compared to platelets from healthy donors. Our assays revealed a set of 252 differentially abundant proteins. In silico analyses associated these proteins with key molecular events including platelet activation and inflammatory responses, and with events not previously attributed to platelets during dengue infection including antigen processing and presentation, proteasome activity, and expression of histones. From these results, we conducted functional assays using samples from a larger cohort of patients and demonstrated evidence for platelet activation indicated by P-selectin (CD62P) translocation and secretion of granule-stored chemokines by platelets. In addition, we found evidence that DENV infection triggers HLA class I synthesis and surface expression by a mechanism depending on functional proteasome activity. Furthermore, we demonstrate that cell-free histone H2A released during dengue infection binds to platelets, increasing platelet activation. These findings are consistent with functional importance of HLA class I, proteasome subunits, and histones that we found exclusively in proteome analysis of platelets in samples from dengue patients. Our study provides the first in-depth characterization of the platelet proteome in dengue, and sheds light on new mechanisms of platelet activation and platelet-mediated immune and inflammatory responses.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20082/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmonique_trugilho_etal_IOC_2017.pdfmonique_trugilho_etal_IOC_2017.pdfapplication/pdf9884296https://www.arca.fiocruz.br/bitstream/icict/20082/2/monique_trugilho_etal_IOC_2017.pdffeaf3f995f51716063abd18ee5752cc1MD52TEXTmonique_trugilho_etal_IOC_2017.pdf.txtmonique_trugilho_etal_IOC_2017.pdf.txtExtracted texttext/plain92018https://www.arca.fiocruz.br/bitstream/icict/20082/3/monique_trugilho_etal_IOC_2017.pdf.txt91162a8aab265440e6cac419aff3e984MD53icict/200822023-09-04 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dc.title.pt_BR.fl_str_mv Platelet proteome reveals novel pathways of platelet activation and platelet-mediated immunoregulation in dengue
title Platelet proteome reveals novel pathways of platelet activation and platelet-mediated immunoregulation in dengue
spellingShingle Platelet proteome reveals novel pathways of platelet activation and platelet-mediated immunoregulation in dengue
Trugilho, Monique Ramos de Oliveira
Dengue
Ativação plaquetária
Immunoregulation
Proteoma
Dengue
Platelet proteome
Immunoregulation
Platelet activation
title_short Platelet proteome reveals novel pathways of platelet activation and platelet-mediated immunoregulation in dengue
title_full Platelet proteome reveals novel pathways of platelet activation and platelet-mediated immunoregulation in dengue
title_fullStr Platelet proteome reveals novel pathways of platelet activation and platelet-mediated immunoregulation in dengue
title_full_unstemmed Platelet proteome reveals novel pathways of platelet activation and platelet-mediated immunoregulation in dengue
title_sort Platelet proteome reveals novel pathways of platelet activation and platelet-mediated immunoregulation in dengue
author Trugilho, Monique Ramos de Oliveira
author_facet Trugilho, Monique Ramos de Oliveira
Hottz, Eugenio Damaceno
Brunoro, Giselle Villa Flor
Teixeira-Ferreira, André
Carvalho, Paulo Costa
Salazar, Gustavo Adolfo
Zimmerman, Guy A.
Bozza, Fernando A.
Bozza, Patrícia Torres
Perales, Jonas
author_role author
author2 Hottz, Eugenio Damaceno
Brunoro, Giselle Villa Flor
Teixeira-Ferreira, André
Carvalho, Paulo Costa
Salazar, Gustavo Adolfo
Zimmerman, Guy A.
Bozza, Fernando A.
Bozza, Patrícia Torres
Perales, Jonas
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Trugilho, Monique Ramos de Oliveira
Hottz, Eugenio Damaceno
Brunoro, Giselle Villa Flor
Teixeira-Ferreira, André
Carvalho, Paulo Costa
Salazar, Gustavo Adolfo
Zimmerman, Guy A.
Bozza, Fernando A.
Bozza, Patrícia Torres
Perales, Jonas
dc.subject.other.pt_BR.fl_str_mv Dengue
Ativação plaquetária
Immunoregulation
Proteoma
topic Dengue
Ativação plaquetária
Immunoregulation
Proteoma
Dengue
Platelet proteome
Immunoregulation
Platelet activation
dc.subject.en.pt_BR.fl_str_mv Dengue
Platelet proteome
Immunoregulation
Platelet activation
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-07-13T17:33:00Z
dc.date.available.fl_str_mv 2017-07-13T17:33:00Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TRUGILHO, Monique Ramos de Oliveira et al. Platelet proteome reveals novel pathways of platelet activation and platelet-mediated immunoregulation in dengue. PLoS Pathogens, v. 13, n. 5, p. 1-27, May 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20082
dc.identifier.issn.pt_BR.fl_str_mv 1553-7366
dc.identifier.doi.none.fl_str_mv 10.1371/journal.ppat.1006385
identifier_str_mv TRUGILHO, Monique Ramos de Oliveira et al. Platelet proteome reveals novel pathways of platelet activation and platelet-mediated immunoregulation in dengue. PLoS Pathogens, v. 13, n. 5, p. 1-27, May 2017.
1553-7366
10.1371/journal.ppat.1006385
url https://www.arca.fiocruz.br/handle/icict/20082
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/20082/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/20082/2/monique_trugilho_etal_IOC_2017.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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