Non-clonal occurrence of pmrB mutations associated with polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae in Brazil

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Ana Claudia Souza
Data de Publicação: 2019
Outros Autores: Santos, Ivson Cassiano de Oliveira, Campos, Caroline Conci, Rezende, Isadora Nascimento, Ferreira, Yanara Miranda, Chaves, Claudia Elisabeth Volpe, Souza, Cláudio Marcos Rocha de, Carvalho-Assef, Ana Paula D’Alincourt, Chang, Marilene Rodrigues
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/35349
Resumo: Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina. Programa de Pós-Graduação em Saúde e Desenvolvimento na Região Centro-Oeste. Campo Grande, MS, Brasil / Universidade Anhanguera Uniderp. Faculdade de Medicina. Campo Grande, MS, Brasil.
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spelling Rodrigues, Ana Claudia SouzaSantos, Ivson Cassiano de OliveiraCampos, Caroline ConciRezende, Isadora NascimentoFerreira, Yanara MirandaChaves, Claudia Elisabeth VolpeSouza, Cláudio Marcos Rocha deCarvalho-Assef, Ana Paula D’AlincourtChang, Marilene Rodrigues2019-09-05T12:47:06Z2019-09-05T12:47:06Z2019RODRIGUES, Ana Claudia Souza et al. Non-clonal occurrence of pmrB mutations associated with polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae in Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-6, 2019.0074-0276https://www.arca.fiocruz.br/handle/icict/3534910.1590/0074-027601805551678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzNon-clonal occurrence of pmrB mutations associated with polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Mato Grosso do Sul. Faculdade de Medicina. Programa de Pós-Graduação em Saúde e Desenvolvimento na Região Centro-Oeste. Campo Grande, MS, Brasil / Universidade Anhanguera Uniderp. Faculdade de Medicina. Campo Grande, MS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.Universidade Federal de Mato Grosso do Sul. Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição. Campo Grande, MS, Brasil.Universidade Federal de Mato Grosso do Sul. Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição. Campo Grande, MS, Brasil.Universidade Federal de Mato Grosso do Sul. Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição. Campo Grande, MS, Brasil.Hospital Regional de Mato Grosso do Sul. Campo Grande, MS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina. Programa de Pós-Graduação em Saúde e Desenvolvimento na Região Centro-Oeste. Campo Grande, MS, Brasil / Universidade Federal de Mato Grosso do Sul. Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição. Campo Grande, MS, Brasil.BACKGROUND Polymyxins are currently used as a “last-line” treatment for multidrug-resistant Gram-negative infections. OBJECTIVES To identify the major mechanisms of resistance to polymyxin and compare the genetic similarity between multidrug resistant Klebsiella pneumoniae strains recovered from inpatients of public hospitals in the Mid-West of Brazil. METHODS 97 carbapenems non-susceptible K. pneumoniae w ere s tudied. β -lactamases ( blaOXA-48, blaKPC, blaNDM, blaCTX-M, blaSHV, blaTEM, blaIMP, blaVIM) and mcr-1 to mcr-5 genes were investigated by polymerase chain reaction (PCR). Mutations in chromosomal genes (pmrA, pmrB, phoP, phoQ, and mgrB) were screened by PCR and DNA sequencing. Clonal relatedness was established by using pulsed-field gel electrophoresis and multilocus sequence typing. FINDINGS K. pneumoniae isolates harbored blaKPC (93.3%), blaSHV (86.6%), blaTEM (80.0%), blaCTX-M (60%) genes. Of 15 K. pneumoniae resistant to polymyxin B the authors identified deleterious mutations in pmrB gene, mainly in T157P. None K. pneumoniae presented mcr gene variants. Genetic polymorphism analyses revealed 12 different pulsotypes. MAIN CONCLUSIONS Deleterious mutations in pmrB gene is the main chromosomal target for induction of polymyxin resistance in carbapenem-resistant K. pneumoniae in public hospitals in the Mid-West of Brazil.ColistinPolymyxinsMultidrug resistanceColistinaPolimixinasResistência a Múltiplos Medicamentosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/35349/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALClaudioMSouza_IvsonSantos_etal_IOC_2019.pdfClaudioMSouza_IvsonSantos_etal_IOC_2019.pdfapplication/pdf347824https://www.arca.fiocruz.br/bitstream/icict/35349/2/ClaudioMSouza_IvsonSantos_etal_IOC_2019.pdf09fa1779688971285108229311f10882MD52TEXTClaudioMSouza_IvsonSantos_etal_IOC_2019.pdf.txtClaudioMSouza_IvsonSantos_etal_IOC_2019.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Non-clonal occurrence of pmrB mutations associated with polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae in Brazil
title Non-clonal occurrence of pmrB mutations associated with polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae in Brazil
spellingShingle Non-clonal occurrence of pmrB mutations associated with polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae in Brazil
Rodrigues, Ana Claudia Souza
Colistin
Polymyxins
Multidrug resistance
Colistina
Polimixinas
Resistência a Múltiplos Medicamentos
title_short Non-clonal occurrence of pmrB mutations associated with polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae in Brazil
title_full Non-clonal occurrence of pmrB mutations associated with polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae in Brazil
title_fullStr Non-clonal occurrence of pmrB mutations associated with polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae in Brazil
title_full_unstemmed Non-clonal occurrence of pmrB mutations associated with polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae in Brazil
title_sort Non-clonal occurrence of pmrB mutations associated with polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae in Brazil
author Rodrigues, Ana Claudia Souza
author_facet Rodrigues, Ana Claudia Souza
Santos, Ivson Cassiano de Oliveira
Campos, Caroline Conci
Rezende, Isadora Nascimento
Ferreira, Yanara Miranda
Chaves, Claudia Elisabeth Volpe
Souza, Cláudio Marcos Rocha de
Carvalho-Assef, Ana Paula D’Alincourt
Chang, Marilene Rodrigues
author_role author
author2 Santos, Ivson Cassiano de Oliveira
Campos, Caroline Conci
Rezende, Isadora Nascimento
Ferreira, Yanara Miranda
Chaves, Claudia Elisabeth Volpe
Souza, Cláudio Marcos Rocha de
Carvalho-Assef, Ana Paula D’Alincourt
Chang, Marilene Rodrigues
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rodrigues, Ana Claudia Souza
Santos, Ivson Cassiano de Oliveira
Campos, Caroline Conci
Rezende, Isadora Nascimento
Ferreira, Yanara Miranda
Chaves, Claudia Elisabeth Volpe
Souza, Cláudio Marcos Rocha de
Carvalho-Assef, Ana Paula D’Alincourt
Chang, Marilene Rodrigues
dc.subject.en.pt_BR.fl_str_mv Colistin
Polymyxins
Multidrug resistance
topic Colistin
Polymyxins
Multidrug resistance
Colistina
Polimixinas
Resistência a Múltiplos Medicamentos
dc.subject.decs.pt_BR.fl_str_mv Colistina
Polimixinas
Resistência a Múltiplos Medicamentos
description Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina. Programa de Pós-Graduação em Saúde e Desenvolvimento na Região Centro-Oeste. Campo Grande, MS, Brasil / Universidade Anhanguera Uniderp. Faculdade de Medicina. Campo Grande, MS, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-09-05T12:47:06Z
dc.date.available.fl_str_mv 2019-09-05T12:47:06Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RODRIGUES, Ana Claudia Souza et al. Non-clonal occurrence of pmrB mutations associated with polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae in Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-6, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/35349
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760180555
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv RODRIGUES, Ana Claudia Souza et al. Non-clonal occurrence of pmrB mutations associated with polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae in Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-6, 2019.
0074-0276
10.1590/0074-02760180555
1678-8060
url https://www.arca.fiocruz.br/handle/icict/35349
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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