The involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosis

Detalhes bibliográficos
Autor(a) principal: Mimura, Kallyne K.
Data de Publicação: 2012
Outros Autores: Tedesco, Roberto C., Calabrese, Katia S., Gil, Cristiane D., Oliani, Sonia M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18335
Resumo: Universidade Federal de São Paulo. Programa de Pós-Graudação em Biologia Funcional e Estrutural. São Paulo, SP, Brasil.
id CRUZ_0195caab9675ee06e648511bf3d02d42
oai_identifier_str oai:www.arca.fiocruz.br:icict/18335
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Mimura, Kallyne K.Tedesco, Roberto C.Calabrese, Katia S.Gil, Cristiane D.Oliani, Sonia M.2017-04-09T17:18:09Z2017-04-09T17:18:09Z2012MIMURA, Kallyne K. et al. The involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosis. Molecular Vision, v.18, p.1583-1593, June 2012.1090-0535https://www.arca.fiocruz.br/handle/icict/18335engMolecular VisionToxoplasmose ocularProteína anti-inflamatóriaAnnexin A1ocular toxoplasmosisanti-inflammatory proteinAnnexin A1The involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Paulo. Programa de Pós-Graudação em Biologia Funcional e Estrutural. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Departamento de Morfologia e Genética. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Universidade Federal de São Paulo. Departamento de Morfologia e Genética. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Programa de Pós-Graduação em Biologia Funcional e Estrutural. São Paulo, SP, Brasil / Universidade Estadual de São Paulo. Instituto de Biociências, Letras e Ciências Exatas. Departamento de Biologia. São José do Rio Preto, SP, BrasilPurpose: The aim of this study was to evaluate the expression of the protein annexin A1 (ANXA1), a potent endogenous regulator of the inflammatory process, in ocular toxoplasmosis. Methods: C57BL/6 female mice were infected using intravitreal injections of either 106 tachyzoites of Toxoplasma gondii (RH strain; T. gondii) or PBS only (control groups). After 24, 48, and 72 h, animals were sacrificed and their eyes were harvested for histopathological, immunohistochemical, and ultrastructural immunocytochemical analysis of ANXA1. Human retinal pigment epithelial (RPE) cells (ARPE-19) were infected in vitro with T. gondii and collected after 60, 120, 240 min, and 24 h. Results: Compared with non-infected eyes, an intense inflammatory response was observed in the anterior (24 h after infection) and posterior segments (72 h after infection) of the infected eye, characterized by neutrophil infiltration and by the presence of tachyzoites and their consequent destruction along with disorganization of normal retina architecture and RPE vacuolization. T. gondii infection was associated with a significant increase of ANXA1 expression in the neutrophils at 24, 48, and 72 h, and in the RPE at 48 and 72 h. In vitro studies confirmed an upregulation of ANXA1 levels in RPE cells, after 60 and 120 min of infection with T. gondii. Conclusions: The positive modulation of endogenous ANXA1 in the inflammatory and RPE cells during T. gondii infection suggests that this protein may serve as a therapeutic target in ocular toxoplasmosis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18335/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALkatia_calabrese_etal_IOC_2012.pdfkatia_calabrese_etal_IOC_2012.pdfapplication/pdf6751028https://www.arca.fiocruz.br/bitstream/icict/18335/2/katia_calabrese_etal_IOC_2012.pdfe7a2bd4db3456448d8651aff48dfef0dMD52TEXTkatia_calabrese_etal_IOC_2012.pdf.txtkatia_calabrese_etal_IOC_2012.pdf.txtExtracted texttext/plain39717https://www.arca.fiocruz.br/bitstream/icict/18335/3/katia_calabrese_etal_IOC_2012.pdf.txtc900867c798ae650749c793a87487cd7MD53icict/183352018-08-15 02:45:41.829oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-08-15T05:45:41Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv The involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosis
title The involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosis
spellingShingle The involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosis
Mimura, Kallyne K.
Toxoplasmose ocular
Proteína anti-inflamatória
Annexin A1
ocular toxoplasmosis
anti-inflammatory protein
Annexin A1
title_short The involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosis
title_full The involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosis
title_fullStr The involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosis
title_full_unstemmed The involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosis
title_sort The involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosis
author Mimura, Kallyne K.
author_facet Mimura, Kallyne K.
Tedesco, Roberto C.
Calabrese, Katia S.
Gil, Cristiane D.
Oliani, Sonia M.
author_role author
author2 Tedesco, Roberto C.
Calabrese, Katia S.
Gil, Cristiane D.
Oliani, Sonia M.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Mimura, Kallyne K.
Tedesco, Roberto C.
Calabrese, Katia S.
Gil, Cristiane D.
Oliani, Sonia M.
dc.subject.other.pt_BR.fl_str_mv Toxoplasmose ocular
Proteína anti-inflamatória
Annexin A1
topic Toxoplasmose ocular
Proteína anti-inflamatória
Annexin A1
ocular toxoplasmosis
anti-inflammatory protein
Annexin A1
dc.subject.en.pt_BR.fl_str_mv ocular toxoplasmosis
anti-inflammatory protein
Annexin A1
description Universidade Federal de São Paulo. Programa de Pós-Graudação em Biologia Funcional e Estrutural. São Paulo, SP, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2017-04-09T17:18:09Z
dc.date.available.fl_str_mv 2017-04-09T17:18:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MIMURA, Kallyne K. et al. The involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosis. Molecular Vision, v.18, p.1583-1593, June 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18335
dc.identifier.issn.pt_BR.fl_str_mv 1090-0535
identifier_str_mv MIMURA, Kallyne K. et al. The involvement of anti-inflammatory protein, Annexin A1, in ocular toxoplasmosis. Molecular Vision, v.18, p.1583-1593, June 2012.
1090-0535
url https://www.arca.fiocruz.br/handle/icict/18335
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Molecular Vision
publisher.none.fl_str_mv Molecular Vision
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/18335/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/18335/2/katia_calabrese_etal_IOC_2012.pdf
https://www.arca.fiocruz.br/bitstream/icict/18335/3/katia_calabrese_etal_IOC_2012.pdf.txt
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
e7a2bd4db3456448d8651aff48dfef0d
c900867c798ae650749c793a87487cd7
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798325008444948480

Registros relacionados