Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.micpath.2018.07.005 http://hdl.handle.net/11449/176576 |
Resumo: | This study was conducted to investigate annexin A1 (ANXA1) functions in human placental explants infected with Toxoplasma gondii (T. gondii). We examined the first and third trimester placental explants infected with T. gondii (n = 7 placentas/group) to identify the number and location of parasites, ANXA1 protein, potential involvement of formyl peptide receptors (FPR1 and FPR2), and COX-2 expressions by immunohistochemistry. Treatments with Ac2-26 mimetic peptide of ANXA1 were performed to verify the parasitism rate (β-galactosidase assay), prostaglandin E2 levels (ELISA assay), and ANXA1, FPR1 and COX-2 expression in third trimester placentas. Placental explants of third trimester expressed less ANXA1 and were more permissive to T. gondii infection than first trimester placentas that expressed more ANXA1. Ac2-26 treatment increases endogenous ANXA1 and decreases parasitism rate, COX-2, and prostaglandin E2 levels. Altogether, these data provide further insight into the anti-parasitic and anti-inflammatory effects of ANXA1 in placentas infected with T. gondii. |
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Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondiiAc2-26 peptideAnnexin A1PlacentaToxoplasmosisThis study was conducted to investigate annexin A1 (ANXA1) functions in human placental explants infected with Toxoplasma gondii (T. gondii). We examined the first and third trimester placental explants infected with T. gondii (n = 7 placentas/group) to identify the number and location of parasites, ANXA1 protein, potential involvement of formyl peptide receptors (FPR1 and FPR2), and COX-2 expressions by immunohistochemistry. Treatments with Ac2-26 mimetic peptide of ANXA1 were performed to verify the parasitism rate (β-galactosidase assay), prostaglandin E2 levels (ELISA assay), and ANXA1, FPR1 and COX-2 expression in third trimester placentas. Placental explants of third trimester expressed less ANXA1 and were more permissive to T. gondii infection than first trimester placentas that expressed more ANXA1. Ac2-26 treatment increases endogenous ANXA1 and decreases parasitism rate, COX-2, and prostaglandin E2 levels. Altogether, these data provide further insight into the anti-parasitic and anti-inflammatory effects of ANXA1 in placentas infected with T. gondii.Post-Graduation in Structural and Functional Biology Federal University of São Paulo (UNIFESP)Faceres School of MedicineDepartment of Structural Biology Institute of Biological and Natural Sciences Federal University of Triângulo Mineiro (UFTM)Department of Biology Instituto de Biociências Letras e Ciências Exatas São Paulo State University (UNESP)Department of Life Sciences University of SienaDepartment of Immunology Microbiology and Parasitology Federal University of Uberlândia (UFU)Department of Histology and Embryology Federal University of Uberlândia (UFU)Department of Biology Instituto de Biociências Letras e Ciências Exatas São Paulo State University (UNESP)Universidade de São Paulo (USP)Faceres School of MedicineFederal University of Triângulo Mineiro (UFTM)Universidade Estadual Paulista (Unesp)University of SienaUniversidade Federal de Uberlândia (UFU)de Oliveira Cardoso, Marystela FáveroMoreli, Jusciele BroginGomes, Angélica Oliveirade Freitas Zanon, Caroline [UNESP]Silva, Ana Elizabete [UNESP]Paulesu, Luana RicciIetta, FrancescaMineo, José RobertoFerro, Eloísa AmáliaOliani, Sonia Maria [UNESP]2018-12-11T17:21:25Z2018-12-11T17:21:25Z2018-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article153-161application/pdfhttp://dx.doi.org/10.1016/j.micpath.2018.07.005Microbial Pathogenesis, v. 123, p. 153-161.1096-12080882-4010http://hdl.handle.net/11449/17657610.1016/j.micpath.2018.07.0052-s2.0-850497446502-s2.0-85049744650.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobial Pathogenesis0,7510,751info:eu-repo/semantics/openAccess2024-01-11T06:27:04Zoai:repositorio.unesp.br:11449/176576Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:40:26.025814Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii |
title |
Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii |
spellingShingle |
Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii de Oliveira Cardoso, Marystela Fávero Ac2-26 peptide Annexin A1 Placenta Toxoplasmosis |
title_short |
Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii |
title_full |
Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii |
title_fullStr |
Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii |
title_full_unstemmed |
Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii |
title_sort |
Annexin A1 peptide is able to induce an anti-parasitic effect in human placental explants infected by Toxoplasma gondii |
author |
de Oliveira Cardoso, Marystela Fávero |
author_facet |
de Oliveira Cardoso, Marystela Fávero Moreli, Jusciele Brogin Gomes, Angélica Oliveira de Freitas Zanon, Caroline [UNESP] Silva, Ana Elizabete [UNESP] Paulesu, Luana Ricci Ietta, Francesca Mineo, José Roberto Ferro, Eloísa Amália Oliani, Sonia Maria [UNESP] |
author_role |
author |
author2 |
Moreli, Jusciele Brogin Gomes, Angélica Oliveira de Freitas Zanon, Caroline [UNESP] Silva, Ana Elizabete [UNESP] Paulesu, Luana Ricci Ietta, Francesca Mineo, José Roberto Ferro, Eloísa Amália Oliani, Sonia Maria [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Faceres School of Medicine Federal University of Triângulo Mineiro (UFTM) Universidade Estadual Paulista (Unesp) University of Siena Universidade Federal de Uberlândia (UFU) |
dc.contributor.author.fl_str_mv |
de Oliveira Cardoso, Marystela Fávero Moreli, Jusciele Brogin Gomes, Angélica Oliveira de Freitas Zanon, Caroline [UNESP] Silva, Ana Elizabete [UNESP] Paulesu, Luana Ricci Ietta, Francesca Mineo, José Roberto Ferro, Eloísa Amália Oliani, Sonia Maria [UNESP] |
dc.subject.por.fl_str_mv |
Ac2-26 peptide Annexin A1 Placenta Toxoplasmosis |
topic |
Ac2-26 peptide Annexin A1 Placenta Toxoplasmosis |
description |
This study was conducted to investigate annexin A1 (ANXA1) functions in human placental explants infected with Toxoplasma gondii (T. gondii). We examined the first and third trimester placental explants infected with T. gondii (n = 7 placentas/group) to identify the number and location of parasites, ANXA1 protein, potential involvement of formyl peptide receptors (FPR1 and FPR2), and COX-2 expressions by immunohistochemistry. Treatments with Ac2-26 mimetic peptide of ANXA1 were performed to verify the parasitism rate (β-galactosidase assay), prostaglandin E2 levels (ELISA assay), and ANXA1, FPR1 and COX-2 expression in third trimester placentas. Placental explants of third trimester expressed less ANXA1 and were more permissive to T. gondii infection than first trimester placentas that expressed more ANXA1. Ac2-26 treatment increases endogenous ANXA1 and decreases parasitism rate, COX-2, and prostaglandin E2 levels. Altogether, these data provide further insight into the anti-parasitic and anti-inflammatory effects of ANXA1 in placentas infected with T. gondii. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:21:25Z 2018-12-11T17:21:25Z 2018-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.micpath.2018.07.005 Microbial Pathogenesis, v. 123, p. 153-161. 1096-1208 0882-4010 http://hdl.handle.net/11449/176576 10.1016/j.micpath.2018.07.005 2-s2.0-85049744650 2-s2.0-85049744650.pdf |
url |
http://dx.doi.org/10.1016/j.micpath.2018.07.005 http://hdl.handle.net/11449/176576 |
identifier_str_mv |
Microbial Pathogenesis, v. 123, p. 153-161. 1096-1208 0882-4010 10.1016/j.micpath.2018.07.005 2-s2.0-85049744650 2-s2.0-85049744650.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microbial Pathogenesis 0,751 0,751 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
153-161 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129450019127296 |