MDL28170, a calpain inhibitor, affects Trypanosoma cruzi metacyclogenesis, ultrastructure and attachment to Rhodnius prolixus midgut

Detalhes bibliográficos
Autor(a) principal: Vidal, Vítor Ennes
Data de Publicação: 2011
Outros Autores: Menna-Barreto, Rubem F. S., Santos, André L. S., Branquinha, Marta H., d`Avila-Levy, Claudia M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15956
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
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spelling Vidal, Vítor EnnesMenna-Barreto, Rubem F. S.Santos, André L. S.Branquinha, Marta H.d`Avila-Levy, Claudia M.2016-09-27T12:01:59Z2016-09-27T12:01:59Z2011VIDAL, Vitor Ennes; et al. MDL28170, a Calpain Inhibitor, Affects Trypanosoma cruzi Metacyclogenesis, Ultrastructure and Attachment to Rhodnius prolixus Midgut. Plos One, v.6, n.4, e18371, 9p, Apr. 2011.1932-6203https://www.arca.fiocruz.br/handle/icict/1595610.1371/journal.pone.0018371engBioMed CentralTrypanosoma cruziDoença de ChagasCalpaínaTrypanosoma cruziChagas DiseaseRhodnius prolixusCalpain InhibitorMDL28170, a calpain inhibitor, affects Trypanosoma cruzi metacyclogenesis, ultrastructure and attachment to Rhodnius prolixus midgutinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Microbiologia Prof. Paulo de Góes (IMPPG). Departamento de Microbiologia Geral. Laboratório de Estudos Integrados em Bioquímica Microbiana. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Microbiologia Prof. Paulo de Góes (IMPPG). Departamento de Microbiologia Geral. Laboratório de Bioquímoca de Proteases. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Background: Trypanosoma cruzi is the etiological agent of Chagas’ disease. During the parasite life cycle, many molecules are involved in the differentiation process and infectivity. Peptidases are relevant for crucial steps of T. cruzi life cycle; as such, it is conceivable that they may participate in the metacyclogenesis and interaction with the invertebrate host. Methodology/Principal Findings: In this paper, we have investigated the effect of the calpain inhibitor MDL28170 on the attachment of T. cruzi epimastigotes to the luminal midgut surface of Rhodnius prolixus, as well as on the metacyclogenesis process and ultrastructure. MDL28170 treatment was capable of significantly reducing the number of bound epimastigotes to the luminal surface midgut of the insect. Once the cross-reactivity of the anti-Dm-calpain was assessed, it was possible to block calpain molecules by the antibody, leading to a significant reduction in the capacity of adhesion to the insect guts by T. cruzi. However, the antibodies were unable to interfere in metacyclogenesis, which was impaired by the calpain inhibitor presenting a significant reduction in the number of metacyclic trypomastigotes. The calpain inhibitor also promoted a direct effect against bloodstream trypomastigotes. Ultrastructural analysis of epimastigotes treated with the calpain inhibitor revealed disorganization in the reservosomes, Golgi and plasma membrane disruption. Conclusions/Significance: The presence of calpain and calpain-like molecules in a wide range of organisms suggests that these proteins could be necessary for basic cellular functions. Herein, we demonstrated the effects of MDL28170 in crucial steps of the T. cruzi life cycle, such as attachment to the insect midgut and metacyclogenesis, as well as in parasite viability and morphology. Together with our previous findings, these results help to shed some light on the functions of T. cruzi calpains. Considering the potential roles of these molecules on the interaction with both invertebrate and vertebrate hosts, it is interesting to improve knowledge on these molecules in T. cruzi.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15956/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALclaudia_levy_etal_IOC_2011.pdfclaudia_levy_etal_IOC_2011.pdfapplication/pdf612277https://www.arca.fiocruz.br/bitstream/icict/15956/2/claudia_levy_etal_IOC_2011.pdf9abfe4ef97bf5346dfd11db3e51f89bcMD52TEXTclaudia_levy_etal_IOC_2011.pdf.txtclaudia_levy_etal_IOC_2011.pdf.txtExtracted texttext/plain49970https://www.arca.fiocruz.br/bitstream/icict/15956/3/claudia_levy_etal_IOC_2011.pdf.txt116ddd85da309e4d08be0e604b571782MD53icict/159562022-06-24 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dc.title.pt_BR.fl_str_mv MDL28170, a calpain inhibitor, affects Trypanosoma cruzi metacyclogenesis, ultrastructure and attachment to Rhodnius prolixus midgut
title MDL28170, a calpain inhibitor, affects Trypanosoma cruzi metacyclogenesis, ultrastructure and attachment to Rhodnius prolixus midgut
spellingShingle MDL28170, a calpain inhibitor, affects Trypanosoma cruzi metacyclogenesis, ultrastructure and attachment to Rhodnius prolixus midgut
Vidal, Vítor Ennes
Trypanosoma cruzi
Doença de Chagas
Calpaína
Trypanosoma cruzi
Chagas Disease
Rhodnius prolixus
Calpain Inhibitor
title_short MDL28170, a calpain inhibitor, affects Trypanosoma cruzi metacyclogenesis, ultrastructure and attachment to Rhodnius prolixus midgut
title_full MDL28170, a calpain inhibitor, affects Trypanosoma cruzi metacyclogenesis, ultrastructure and attachment to Rhodnius prolixus midgut
title_fullStr MDL28170, a calpain inhibitor, affects Trypanosoma cruzi metacyclogenesis, ultrastructure and attachment to Rhodnius prolixus midgut
title_full_unstemmed MDL28170, a calpain inhibitor, affects Trypanosoma cruzi metacyclogenesis, ultrastructure and attachment to Rhodnius prolixus midgut
title_sort MDL28170, a calpain inhibitor, affects Trypanosoma cruzi metacyclogenesis, ultrastructure and attachment to Rhodnius prolixus midgut
author Vidal, Vítor Ennes
author_facet Vidal, Vítor Ennes
Menna-Barreto, Rubem F. S.
Santos, André L. S.
Branquinha, Marta H.
d`Avila-Levy, Claudia M.
author_role author
author2 Menna-Barreto, Rubem F. S.
Santos, André L. S.
Branquinha, Marta H.
d`Avila-Levy, Claudia M.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Vidal, Vítor Ennes
Menna-Barreto, Rubem F. S.
Santos, André L. S.
Branquinha, Marta H.
d`Avila-Levy, Claudia M.
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Doença de Chagas
Calpaína
topic Trypanosoma cruzi
Doença de Chagas
Calpaína
Trypanosoma cruzi
Chagas Disease
Rhodnius prolixus
Calpain Inhibitor
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Chagas Disease
Rhodnius prolixus
Calpain Inhibitor
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2016-09-27T12:01:59Z
dc.date.available.fl_str_mv 2016-09-27T12:01:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VIDAL, Vitor Ennes; et al. MDL28170, a Calpain Inhibitor, Affects Trypanosoma cruzi Metacyclogenesis, Ultrastructure and Attachment to Rhodnius prolixus Midgut. Plos One, v.6, n.4, e18371, 9p, Apr. 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15956
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0018371
identifier_str_mv VIDAL, Vitor Ennes; et al. MDL28170, a Calpain Inhibitor, Affects Trypanosoma cruzi Metacyclogenesis, Ultrastructure and Attachment to Rhodnius prolixus Midgut. Plos One, v.6, n.4, e18371, 9p, Apr. 2011.
1932-6203
10.1371/journal.pone.0018371
url https://www.arca.fiocruz.br/handle/icict/15956
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/15956/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/15956/2/claudia_levy_etal_IOC_2011.pdf
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