Polyunsaturated fatty acids alter the formation of lipid droplets and eicosanoid production in Leishmania promastigotes

Detalhes bibliográficos
Autor(a) principal: Andrade, Yasmin Monara Ferreira de Sousa
Data de Publicação: 2022
Outros Autores: Castro, Monara Viera de, Tavares, Victor de Souza, Souza, Rayane da Silva Oliveira, Faccioli, Lúcia Helena, Lima, Jonilson Berlink, Sorgi, Carlos Arterio, Borges, Valéria M., Araújo-Santos, Théo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/52385
Resumo: Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.
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spelling Andrade, Yasmin Monara Ferreira de SousaCastro, Monara Viera deTavares, Victor de SouzaSouza, Rayane da Silva OliveiraFaccioli, Lúcia HelenaLima, Jonilson BerlinkSorgi, Carlos ArterioBorges, Valéria M.Araújo-Santos, Théo2022-04-23T00:51:46Z2022-04-23T00:51:46Z2023ANDRADE, Yasmin Monara Ferreira de Sousa et al. Polyunsaturated fatty acids alter the formation of lipid droplets and eicosanoid production in Leishmania promastigotes. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 118, p. 1-7, 2023.0074-0276https://www.arca.fiocruz.br/handle/icict/5238510.1590/0074-027602201601678-8060engFiocruz/IOCPolyunsaturated fatty acids alter the formation of lipid droplets and eicosanoid production in Leishmania promastigotesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Universidade Federal do Oeste da Bahia. Centro das Ciências Biológicas e da Saúde. Núcleo de Estudos de Agentes Infecciosos e Vetores. Barreiras, BA, Brasil.Universidade Federal do Oeste da Bahia. Centro das Ciências Biológicas e da Saúde. Núcleo de Estudos de Agentes Infecciosos e Vetores. Barreiras, BA, Brasil.Universidade Federal do Oeste da Bahia. Centro das Ciências Biológicas e da Saúde. Núcleo de Estudos de Agentes Infecciosos e Vetores. Barreiras, BA, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal do Oeste da Bahia. Centro das Ciências Biológicas e da Saúde. Núcleo de Estudos de Agentes Infecciosos e Vetores, Barreiras. BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Universidade de São Paulo. Ribeirão Preto, SP, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal do Oeste da Bahia. Centro das Ciências Biológicas e da Saúde. Núcleo de Estudos de Agentes Infecciosos e Vetores. Barreiras, BA, Brasil.Background: The knowledge about eicosanoid metabolism and lipid droplet (LD) formation in the Leishmania is very limited and new approaches are needed to identify which bioactive molecules are produced of them. Objetives: Herein, we compared LDs and eicosanoids biogenesis in distinct Leishmania species which are etiologic agents of different clinical forms of leishmaniasis. Methods: For this, promastigotes of Leishmania amazonensis, L. braziliensis and L. infantum were stimulated with polyunsaturated fatty acids (PUFA) and LD and eicosanoid production was evaluated. We also compared mutations in structural models of human-like cyclooxygenase-2 (GP63) and prostaglandin F synthase (PGFS) proteins, as well as the levels of these enzymes in parasite cell extracts. Findings: PUFAs modulate the LD formation in L. braziliensis and L. infantum. Leishmania spp with equivalent tissue tropism had same protein mutations in GP63 and PGFS. No differences in GP63 production were observed among Leishmania spp, however PGFS production increased during the parasite differentiation. Stimulation with arachidonic acid resulted in elevated production of hydroxyeicosatetraenoic acids compared to prostaglandins. Main Conclusion: Our data suggest LD formation and eicosanoid production are distinctly modulated by PUFAS dependent of Leishmania species. In addition, eicosanoid-enzyme mutations are more similar between Leishmania species with same host tropism.Lipid dropletEicosanoidPolyunsaturated fatty acidsGP63PGF synthaseLeishmaniainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALve_Yasmin_Andrade_etal_IGM_2023.pdfve_Yasmin_Andrade_etal_IGM_2023.pdfapplication/pdf1124174https://www.arca.fiocruz.br/bitstream/icict/52385/2/ve_Yasmin_Andrade_etal_IGM_2023.pdfff3f6ddeecf62272a5e104e9d8b8e015MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/52385/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/523852023-04-30 22:52:17.755oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-05-01T01:52:17Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Polyunsaturated fatty acids alter the formation of lipid droplets and eicosanoid production in Leishmania promastigotes
title Polyunsaturated fatty acids alter the formation of lipid droplets and eicosanoid production in Leishmania promastigotes
spellingShingle Polyunsaturated fatty acids alter the formation of lipid droplets and eicosanoid production in Leishmania promastigotes
Andrade, Yasmin Monara Ferreira de Sousa
Lipid droplet
Eicosanoid
Polyunsaturated fatty acids
GP63
PGF synthase
Leishmania
title_short Polyunsaturated fatty acids alter the formation of lipid droplets and eicosanoid production in Leishmania promastigotes
title_full Polyunsaturated fatty acids alter the formation of lipid droplets and eicosanoid production in Leishmania promastigotes
title_fullStr Polyunsaturated fatty acids alter the formation of lipid droplets and eicosanoid production in Leishmania promastigotes
title_full_unstemmed Polyunsaturated fatty acids alter the formation of lipid droplets and eicosanoid production in Leishmania promastigotes
title_sort Polyunsaturated fatty acids alter the formation of lipid droplets and eicosanoid production in Leishmania promastigotes
author Andrade, Yasmin Monara Ferreira de Sousa
author_facet Andrade, Yasmin Monara Ferreira de Sousa
Castro, Monara Viera de
Tavares, Victor de Souza
Souza, Rayane da Silva Oliveira
Faccioli, Lúcia Helena
Lima, Jonilson Berlink
Sorgi, Carlos Arterio
Borges, Valéria M.
Araújo-Santos, Théo
author_role author
author2 Castro, Monara Viera de
Tavares, Victor de Souza
Souza, Rayane da Silva Oliveira
Faccioli, Lúcia Helena
Lima, Jonilson Berlink
Sorgi, Carlos Arterio
Borges, Valéria M.
Araújo-Santos, Théo
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Andrade, Yasmin Monara Ferreira de Sousa
Castro, Monara Viera de
Tavares, Victor de Souza
Souza, Rayane da Silva Oliveira
Faccioli, Lúcia Helena
Lima, Jonilson Berlink
Sorgi, Carlos Arterio
Borges, Valéria M.
Araújo-Santos, Théo
dc.subject.en.none.fl_str_mv Lipid droplet
Eicosanoid
Polyunsaturated fatty acids
GP63
PGF synthase
Leishmania
topic Lipid droplet
Eicosanoid
Polyunsaturated fatty acids
GP63
PGF synthase
Leishmania
description Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-04-23T00:51:46Z
dc.date.available.fl_str_mv 2022-04-23T00:51:46Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv ANDRADE, Yasmin Monara Ferreira de Sousa et al. Polyunsaturated fatty acids alter the formation of lipid droplets and eicosanoid production in Leishmania promastigotes. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 118, p. 1-7, 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/52385
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760220160
dc.identifier.eissn.pt_BR.fl_str_mv 1678-8060
identifier_str_mv ANDRADE, Yasmin Monara Ferreira de Sousa et al. Polyunsaturated fatty acids alter the formation of lipid droplets and eicosanoid production in Leishmania promastigotes. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 118, p. 1-7, 2023.
0074-0276
10.1590/0074-02760220160
1678-8060
url https://www.arca.fiocruz.br/handle/icict/52385
dc.language.iso.fl_str_mv eng
language eng
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eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fiocruz/IOC
publisher.none.fl_str_mv Fiocruz/IOC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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