The abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded Type IV Secretion System of Brucella ovis Are Critical for Intracellular Trafficking and Survival in Ovine Monocyte-Derived Macrophages

Detalhes bibliográficos
Autor(a) principal: Macedo, Auricelio Alves
Data de Publicação: 2015
Outros Autores: Silva, Ana Patricia Carvalho, Mol, Juliana Pinto da Silva, Costa, Luciana Fachini, Garcia, Luize Neli Nunes, Araújo, Márcio Sobreira Silva, Martins Filho, Olindo Assis, Paixão, Tatiane Alves da, Santos, Renato Lima
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13066
Resumo: Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinárias. Belo Horizonte, MG, Brasil
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spelling Macedo, Auricelio AlvesSilva, Ana Patricia CarvalhoMol, Juliana Pinto da SilvaCosta, Luciana FachiniGarcia, Luize Neli NunesAraújo, Márcio Sobreira SilvaMartins Filho, Olindo AssisPaixão, Tatiane Alves daSantos, Renato Lima2016-03-09T19:46:41Z2016-03-09T19:46:41Z2015MACEDO, Auricelio Alves et al. The abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded Type IV Secretion System of Brucella ovis Are Critical for Intracellular Trafficking and Survival in Ovine Monocyte-Derived Macrophages. PLoS One. Vol. 10, n. 9, p. e0138131, 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/1306610.1371/journal.pone.0138131engPublic Library of ScienceThe abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded Type IV Secretion System of Brucella ovis Are Critical for Intracellular Trafficking and Survival in Ovine Monocyte-Derived Macrophagesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinárias. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinárias. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinárias. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinárias. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia Geral. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinárias. Belo Horizonte, MG, BrasilBrucella ovis infection is associated with epididymitis, orchitis and infertility in rams. Most of the information available on B. ovis and host cell interaction has been generated using murine macrophages or epithelial cell lines, but the interaction between B. ovis and primary ovine macrophages has not been studied. The aim of this study was to evaluate the role of the B. ovis abcEDCBA-encoded ABC transporter and the virB operon-encoded Type IV Secretion System (T4SS) during intracellular survival of B. ovis in ovine peripheral blood monocyte-derived macrophages. ΔabcBA and ΔvirB2 mutant strains were unable to survive in the intracellular environment when compared to the WT B. ovis at 48 hours post infection (hpi). In addition, these mutant strains cannot exclude the lysosomal marker LAMP1 from its vacuolar membrane, and their vacuoles do not acquire the endoplasmic reticulum marker calreticulin, which takes place in the WT B. ovis containing vacuole. Higher levels of nitric oxide production were observed in macrophages infected with WT B. ovis at 48 hpi when compared to macrophages infected with the ΔabcBA or ΔvirB2 mutant strains. Conversely, higher levels of reactive oxygen species were detected in macrophages infected with the ΔabcBA or ΔvirB2 mutant strains at 48 hpi when compared to macrophages infected with the WT strain. Our results demonstrate that B. ovis is able to persist and multiply in ovine macrophages, while ΔabcBA and ΔvirB2 mutations prevent intracellular multiplication, favor phagolysosome fusion, and impair maturation of the B. ovis vacuole towards an endoplasmic reticulum-derived compartmentBrucella ovisdiseasisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13066/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALhe abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded.pdfhe abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded.pdfapplication/pdf6274993https://www.arca.fiocruz.br/bitstream/icict/13066/2/he%20abcEDCBA-Encoded%20ABC%20Transporter%20and%20the%20virB%20Operon-Encoded.pdff8ae6625f52da4bd6d4d24972ca5def7MD52TEXThe abcEDCBA-Encoded ABC Transporter and the virB 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dc.title.pt_BR.fl_str_mv The abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded Type IV Secretion System of Brucella ovis Are Critical for Intracellular Trafficking and Survival in Ovine Monocyte-Derived Macrophages
title The abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded Type IV Secretion System of Brucella ovis Are Critical for Intracellular Trafficking and Survival in Ovine Monocyte-Derived Macrophages
spellingShingle The abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded Type IV Secretion System of Brucella ovis Are Critical for Intracellular Trafficking and Survival in Ovine Monocyte-Derived Macrophages
Macedo, Auricelio Alves
Brucella ovis
diseasis
title_short The abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded Type IV Secretion System of Brucella ovis Are Critical for Intracellular Trafficking and Survival in Ovine Monocyte-Derived Macrophages
title_full The abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded Type IV Secretion System of Brucella ovis Are Critical for Intracellular Trafficking and Survival in Ovine Monocyte-Derived Macrophages
title_fullStr The abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded Type IV Secretion System of Brucella ovis Are Critical for Intracellular Trafficking and Survival in Ovine Monocyte-Derived Macrophages
title_full_unstemmed The abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded Type IV Secretion System of Brucella ovis Are Critical for Intracellular Trafficking and Survival in Ovine Monocyte-Derived Macrophages
title_sort The abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded Type IV Secretion System of Brucella ovis Are Critical for Intracellular Trafficking and Survival in Ovine Monocyte-Derived Macrophages
author Macedo, Auricelio Alves
author_facet Macedo, Auricelio Alves
Silva, Ana Patricia Carvalho
Mol, Juliana Pinto da Silva
Costa, Luciana Fachini
Garcia, Luize Neli Nunes
Araújo, Márcio Sobreira Silva
Martins Filho, Olindo Assis
Paixão, Tatiane Alves da
Santos, Renato Lima
author_role author
author2 Silva, Ana Patricia Carvalho
Mol, Juliana Pinto da Silva
Costa, Luciana Fachini
Garcia, Luize Neli Nunes
Araújo, Márcio Sobreira Silva
Martins Filho, Olindo Assis
Paixão, Tatiane Alves da
Santos, Renato Lima
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Macedo, Auricelio Alves
Silva, Ana Patricia Carvalho
Mol, Juliana Pinto da Silva
Costa, Luciana Fachini
Garcia, Luize Neli Nunes
Araújo, Márcio Sobreira Silva
Martins Filho, Olindo Assis
Paixão, Tatiane Alves da
Santos, Renato Lima
dc.subject.en.pt_BR.fl_str_mv Brucella ovis
diseasis
topic Brucella ovis
diseasis
description Universidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinárias. Belo Horizonte, MG, Brasil
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-03-09T19:46:41Z
dc.date.available.fl_str_mv 2016-03-09T19:46:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv MACEDO, Auricelio Alves et al. The abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded Type IV Secretion System of Brucella ovis Are Critical for Intracellular Trafficking and Survival in Ovine Monocyte-Derived Macrophages. PLoS One. Vol. 10, n. 9, p. e0138131, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13066
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0138131
identifier_str_mv MACEDO, Auricelio Alves et al. The abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded Type IV Secretion System of Brucella ovis Are Critical for Intracellular Trafficking and Survival in Ovine Monocyte-Derived Macrophages. PLoS One. Vol. 10, n. 9, p. e0138131, 2015.
1932-6203
10.1371/journal.pone.0138131
url https://www.arca.fiocruz.br/handle/icict/13066
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
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