Genotyping of Mycobacterium leprae for better understanding of leprosy transmission in Fortaleza, Northeastern Brazil

Detalhes bibliográficos
Autor(a) principal: Fontes, Amanda N. B.
Data de Publicação: 2017
Outros Autores: Lima, Luana N. G. C., Mota, Rosa M. S., Almeida, Rosa L. F., Pontes, Maria A., Gonçalves, Heitor de S., Frota, Cristiane C., Vissa, Varalakshmi D., Brennan, Patrick J., Guimaraes, Ricardo J. P. S., Kendall, Carl, Kerr, Ligia R. F. S., Suffys, Philip N.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/26755
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada à Micobactérias. Rio de Janeiro, RJ. Brasil.
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spelling Fontes, Amanda N. B.Lima, Luana N. G. C.Mota, Rosa M. S.Almeida, Rosa L. F.Pontes, Maria A.Gonçalves, Heitor de S.Frota, Cristiane C.Vissa, Varalakshmi D.Brennan, Patrick J.Guimaraes, Ricardo J. P. S.Kendall, CarlKerr, Ligia R. F. S.Suffys, Philip N.2018-06-07T11:41:43Z2018-06-07T11:41:43Z2017FONTES, Amanda N. B. et al. Genotyping of Mycobacterium leprae for better understanding of leprosy transmission in Fortaleza, Northeastern Brazil. PLoS Negl Trop Dis., v.11, n.12, e0006117, 20p, Dec. 2017.1935-2727https://www.arca.fiocruz.br/handle/icict/2675510.1371/journal.pntd.00061171935-2735engPublic Library of ScienceHanseníaseMycobacterium lepraeTransmissãogenotipagemCearáLeprosyMycobacterium lepraetransmissionGenotypingBrazilGenotyping of Mycobacterium leprae for better understanding of leprosy transmission in Fortaleza, Northeastern Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada à Micobactérias. Rio de Janeiro, RJ. Brasil.Secretaria de Saúde do Estado do Ceará. Departamento de Patologia. Fortaleza, CE, Brasil.Universidade Federal do Ceará. Departamento de Estatística e Matemática Aplicada. Fortaleza, CE, Brasil.Universidade de Fortaleza. Programa de Pós-Graduação em Saúde Pública. Fortaleza, CE, Brasil.Secretaria de Saúde do Estado do Ceará. Centro de Referência em Dermatologia Dona Libânia. Fortaleza, CE, Brasil.Secretaria de Saúde do Estado do Ceará. Centro de Referência em Dermatologia Dona Libânia. Fortaleza, CE, Brasil.Universidade Federal do Ceará. Departamento de Patologia. Fortaleza, CE, Brasil.Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, Colorado, USA.Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, Colorado, USA.Instituto Evandro Chagas. Laboratório de Geoprocessamento. Belém, PA, Brasil.Tulane School of Public Health and Tropical Medicine. Department of Global Community Health and Behavioral Sciences. New Orleans, USA. / Universidade Federal do Ceará. Escola de Medicina. Departamento de Saúde Comunitária. Fortaleza, CE, Brasil.Universidade Federal do Ceará. Escola de Medicina. Departamento de Saúde Comunitária. Fortaleza, CE, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada à Micobactérias. Rio de Janeiro, RJ. Brasil / Tropical Institute of Medicine. Department of Biomedical Sciences. Mycobacteriology Unit. Antwerp, Belgium.Leprosy is endemic in large part of Brazil with 28,761 new patients in 2015, the second largest number worldwide and reaches 9/10.000 in highly endemic regions and 2.7/10.000 in the city of Fortaleza, Ceará, Northeast Brazil. For better understanding of risk factors for leprosy transmission, we conducted an epidemiologic study supplemented by 17 locus VNTR and SNP 1-4 typing of Mycobacterium leprae in skin biopsy samples from new multibacillary (MB) patients diagnosed at a reference center in 2009 and 2010. Among the 1,519 new patients detected during the study period, 998 (65.7%) were MB and we performed DNA extraction and genotyping on 160 skin biopsy samples, resulting in 159 (16%) good multilocus VNTR types. Thirty-eight of these patients also provided VNTR types from M. leprae in nasal swabs. The SNP-Type was obtained for 157 patients and 87% were of type 4. Upon consideration all VNTR markers, 156 different genotypes and three pairs with identical genotypes were observed; no epidemiologic relation could be observed between individuals in these pairs. Considerable variability in differentiating index (DI) was observed between the different markers and the four with highest DI [(AT)15, (TA)18, (AT)17 and (GAA)21] frequently demonstrated differences in copy number when comparing genotypes from both type of samples. Excluding these markers from analysis resulted in 83 genotypes, 20 of which included 96 of the patients (60.3%). These clusters were composed of two (n = 8), three (n = 6), four (n = 1), five (n = 2), six (n = 1), 19 (n = 1) and 23 (n = 23) individuals and suggests that recent transmission is contributing to the maintenance of leprosy in Fortaleza. When comparing epidemiological and clinical variables among patients within clustered or with unique M. leprae genotypes, a positive bacterial index in skin biopsies and knowledge of working with someone with the disease were significantly associated with clustering. A tendency to belong to a cluster was observed with later notification of disease (mean value of 3.4 months) and having disability grade 2. A tendency for lack of clustering was observed for patients who reported to have lived with another leprosy case but this might be due to lack of inclusion of household contacts in the study. Although clusters were spread over the city, kernel analysis revealed that some of the patients belonging to the two major clusters were spatially related to some neighborhoods that report poverty and high disease incidence in children. Finally, inclusion of genotypes from nasal swabs might be warranted. A major limitation of the study is that sample size of 160 patients from a two year period represents only 15% of the new patients and this could have weakened statistical outcomes. This is the first molecular epidemiology study of leprosy in Brazil and although the high clustering level suggests that recent transmission is the major cause of disease in Fortaleza; the existence of two large clusters needs further investigation.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/26755/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALamanda_fontes_etal_IOC_2017.pdfamanda_fontes_etal_IOC_2017.pdfapplication/pdf4701287https://www.arca.fiocruz.br/bitstream/icict/26755/2/amanda_fontes_etal_IOC_2017.pdfe81bb9d8b6dee76bca288808ea140152MD52TEXTamanda_fontes_etal_IOC_2017.pdf.txtamanda_fontes_etal_IOC_2017.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Genotyping of Mycobacterium leprae for better understanding of leprosy transmission in Fortaleza, Northeastern Brazil
title Genotyping of Mycobacterium leprae for better understanding of leprosy transmission in Fortaleza, Northeastern Brazil
spellingShingle Genotyping of Mycobacterium leprae for better understanding of leprosy transmission in Fortaleza, Northeastern Brazil
Fontes, Amanda N. B.
Hanseníase
Mycobacterium leprae
Transmissão
genotipagem
Ceará
Leprosy
Mycobacterium leprae
transmission
Genotyping
Brazil
title_short Genotyping of Mycobacterium leprae for better understanding of leprosy transmission in Fortaleza, Northeastern Brazil
title_full Genotyping of Mycobacterium leprae for better understanding of leprosy transmission in Fortaleza, Northeastern Brazil
title_fullStr Genotyping of Mycobacterium leprae for better understanding of leprosy transmission in Fortaleza, Northeastern Brazil
title_full_unstemmed Genotyping of Mycobacterium leprae for better understanding of leprosy transmission in Fortaleza, Northeastern Brazil
title_sort Genotyping of Mycobacterium leprae for better understanding of leprosy transmission in Fortaleza, Northeastern Brazil
author Fontes, Amanda N. B.
author_facet Fontes, Amanda N. B.
Lima, Luana N. G. C.
Mota, Rosa M. S.
Almeida, Rosa L. F.
Pontes, Maria A.
Gonçalves, Heitor de S.
Frota, Cristiane C.
Vissa, Varalakshmi D.
Brennan, Patrick J.
Guimaraes, Ricardo J. P. S.
Kendall, Carl
Kerr, Ligia R. F. S.
Suffys, Philip N.
author_role author
author2 Lima, Luana N. G. C.
Mota, Rosa M. S.
Almeida, Rosa L. F.
Pontes, Maria A.
Gonçalves, Heitor de S.
Frota, Cristiane C.
Vissa, Varalakshmi D.
Brennan, Patrick J.
Guimaraes, Ricardo J. P. S.
Kendall, Carl
Kerr, Ligia R. F. S.
Suffys, Philip N.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fontes, Amanda N. B.
Lima, Luana N. G. C.
Mota, Rosa M. S.
Almeida, Rosa L. F.
Pontes, Maria A.
Gonçalves, Heitor de S.
Frota, Cristiane C.
Vissa, Varalakshmi D.
Brennan, Patrick J.
Guimaraes, Ricardo J. P. S.
Kendall, Carl
Kerr, Ligia R. F. S.
Suffys, Philip N.
dc.subject.other.pt_BR.fl_str_mv Hanseníase
Mycobacterium leprae
Transmissão
genotipagem
Ceará
topic Hanseníase
Mycobacterium leprae
Transmissão
genotipagem
Ceará
Leprosy
Mycobacterium leprae
transmission
Genotyping
Brazil
dc.subject.en.pt_BR.fl_str_mv Leprosy
Mycobacterium leprae
transmission
Genotyping
Brazil
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada à Micobactérias. Rio de Janeiro, RJ. Brasil.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-06-07T11:41:43Z
dc.date.available.fl_str_mv 2018-06-07T11:41:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FONTES, Amanda N. B. et al. Genotyping of Mycobacterium leprae for better understanding of leprosy transmission in Fortaleza, Northeastern Brazil. PLoS Negl Trop Dis., v.11, n.12, e0006117, 20p, Dec. 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/26755
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0006117
dc.identifier.eissn.none.fl_str_mv 1935-2735
identifier_str_mv FONTES, Amanda N. B. et al. Genotyping of Mycobacterium leprae for better understanding of leprosy transmission in Fortaleza, Northeastern Brazil. PLoS Negl Trop Dis., v.11, n.12, e0006117, 20p, Dec. 2017.
1935-2727
10.1371/journal.pntd.0006117
1935-2735
url https://www.arca.fiocruz.br/handle/icict/26755
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron_str FIOCRUZ
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bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/26755/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/26755/2/amanda_fontes_etal_IOC_2017.pdf
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