Pathogen-specific epitopes as epidemiological tools for defining the magnitude of Mycobacterium leprae transmission in areas endemic for leprosy

Detalhes bibliográficos
Autor(a) principal: Martins, Marcia V. S. B.
Data de Publicação: 2012
Outros Autores: Guimarães, Marjorie M. da S., Spencer, John S., Hacker, Mariana A.V. B., Costa, Luciana S., Carvalho, Fernanda M., Geluk, Annemieke, van der Ploeg-van Schip, Jolien J., Pontes, Maria A. A., Gonçalves, Heitor S., Morais, Janvier P. de, Bandeira, Tereza J. P. G., Pessolani, Maria C .V., Brennan, Patrick J., Pereira, Geraldo M. B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16792
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
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spelling Martins, Marcia V. S. B.Guimarães, Marjorie M. da S.Spencer, John S.Hacker, Mariana A.V. B.Costa, Luciana S.Carvalho, Fernanda M.Geluk, Annemiekevan der Ploeg-van Schip, Jolien J.Pontes, Maria A. A.Gonçalves, Heitor S.Morais, Janvier P. deBandeira, Tereza J. P. G.Pessolani, Maria C .V.Brennan, Patrick J.Pereira, Geraldo M. B.2017-01-10T22:07:01Z2017-01-10T22:07:01Z2012MARTINS, Márcia V. S. B. et al. Pathogen-Specific Epitopes as Epidemiological Tools for Defining the Magnitude of Mycobacterium leprae transmission in Areas Endemic for Leprosy. Plos Negl Trop Dis., v.6, n.4, e1616, 12p, Apr. 2012.1935-2727https://www.arca.fiocruz.br/handle/icict/1679210.1371/journal.pntd.00016161935-2735engPublic Library of ScienceHanseníaseÁreas endêmicasTransmissãoMycobacterium lepraeEpitoposMycobacterium lepraeLeprosyAreas EndemicTransmissionPathogen-Specific EpitopesPathogen-specific epitopes as epidemiological tools for defining the magnitude of Mycobacterium leprae transmission in areas endemic for leprosyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, Colorado, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Curz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Escola de Ciências Médicas. Laboratório de Informática Médica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Leiden University Medical Center. Department of Infectious Diseases. Leiden, The Netherlands.Leiden University Medical Center. Department of Infectious Diseases. Leiden, The Netherlands.Centro de Referência Dona Libania. Fortaleza, CE, Brasil.Centro de Referência Dona Libania. Fortaleza, CE, Brasil.SER V. Programa de Controle de Hanseníase. Fortaleza, CE, BrasilLabPasteur Diagnostic Medicine / Diagnostics of America (DASA), Fortaleza, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, Colorado, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Escola de Ciências Médicas. Laboratório de Imunopatologia. Rio de Janeiro, RJ, Brasil.During recent years, comparative genomic analysis has allowed the identification of Mycobacterium leprae-specific genes with potential application for the diagnosis of leprosy. In a previous study, 58 synthetic peptides derived from these sequences were tested for their ability to induce production of IFN-γ in PBMC from endemic controls (EC) with unknown exposure to M. leprae, household contacts of leprosy patients and patients, indicating the potential of these synthetic peptides for the diagnosis of sub- or preclinical forms of leprosy. In the present study, the patterns of IFN-γ release of the individuals exposed or non-exposed to M. leprae were compared using an Artificial Neural Network algorithm, and the most promising M. leprae peptides for the identification of exposed people were selected. This subset of M. leprae-specific peptides allowed the differentiation of groups of individuals from sites hyperendemic for leprosy versus those from areas with lower level detection rates. A progressive reduction in the IFN-γ levels in response to the peptides was seen when contacts of multibacillary (MB) patients were compared to other less exposed groups, suggesting a down modulation of IFN-γ production with an increase in bacillary load or exposure to M. leprae. The data generated indicate that an IFN-γ assay based on these peptides applied individually or as a pool can be used as a new tool for predicting the magnitude of M. leprae transmission in a given population.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16792/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmariana2_hacker_etal_IOC_2012.pdfmariana2_hacker_etal_IOC_2012.pdfapplication/pdf2083353https://www.arca.fiocruz.br/bitstream/icict/16792/2/mariana2_hacker_etal_IOC_2012.pdf14e2d553c3640bcce36cc3d8cb3c688dMD52TEXTmariana2_hacker_etal_IOC_2012.pdf.txtmariana2_hacker_etal_IOC_2012.pdf.txtExtracted texttext/plain52760https://www.arca.fiocruz.br/bitstream/icict/16792/3/mariana2_hacker_etal_IOC_2012.pdf.txt8cd3b764222e707f985b91967b54b239MD53icict/167922023-09-14 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dc.title.pt_BR.fl_str_mv Pathogen-specific epitopes as epidemiological tools for defining the magnitude of Mycobacterium leprae transmission in areas endemic for leprosy
title Pathogen-specific epitopes as epidemiological tools for defining the magnitude of Mycobacterium leprae transmission in areas endemic for leprosy
spellingShingle Pathogen-specific epitopes as epidemiological tools for defining the magnitude of Mycobacterium leprae transmission in areas endemic for leprosy
Martins, Marcia V. S. B.
Hanseníase
Áreas endêmicas
Transmissão
Mycobacterium leprae
Epitopos
Mycobacterium leprae
Leprosy
Areas Endemic
Transmission
Pathogen-Specific Epitopes
title_short Pathogen-specific epitopes as epidemiological tools for defining the magnitude of Mycobacterium leprae transmission in areas endemic for leprosy
title_full Pathogen-specific epitopes as epidemiological tools for defining the magnitude of Mycobacterium leprae transmission in areas endemic for leprosy
title_fullStr Pathogen-specific epitopes as epidemiological tools for defining the magnitude of Mycobacterium leprae transmission in areas endemic for leprosy
title_full_unstemmed Pathogen-specific epitopes as epidemiological tools for defining the magnitude of Mycobacterium leprae transmission in areas endemic for leprosy
title_sort Pathogen-specific epitopes as epidemiological tools for defining the magnitude of Mycobacterium leprae transmission in areas endemic for leprosy
author Martins, Marcia V. S. B.
author_facet Martins, Marcia V. S. B.
Guimarães, Marjorie M. da S.
Spencer, John S.
Hacker, Mariana A.V. B.
Costa, Luciana S.
Carvalho, Fernanda M.
Geluk, Annemieke
van der Ploeg-van Schip, Jolien J.
Pontes, Maria A. A.
Gonçalves, Heitor S.
Morais, Janvier P. de
Bandeira, Tereza J. P. G.
Pessolani, Maria C .V.
Brennan, Patrick J.
Pereira, Geraldo M. B.
author_role author
author2 Guimarães, Marjorie M. da S.
Spencer, John S.
Hacker, Mariana A.V. B.
Costa, Luciana S.
Carvalho, Fernanda M.
Geluk, Annemieke
van der Ploeg-van Schip, Jolien J.
Pontes, Maria A. A.
Gonçalves, Heitor S.
Morais, Janvier P. de
Bandeira, Tereza J. P. G.
Pessolani, Maria C .V.
Brennan, Patrick J.
Pereira, Geraldo M. B.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Martins, Marcia V. S. B.
Guimarães, Marjorie M. da S.
Spencer, John S.
Hacker, Mariana A.V. B.
Costa, Luciana S.
Carvalho, Fernanda M.
Geluk, Annemieke
van der Ploeg-van Schip, Jolien J.
Pontes, Maria A. A.
Gonçalves, Heitor S.
Morais, Janvier P. de
Bandeira, Tereza J. P. G.
Pessolani, Maria C .V.
Brennan, Patrick J.
Pereira, Geraldo M. B.
dc.subject.other.pt_BR.fl_str_mv Hanseníase
Áreas endêmicas
Transmissão
Mycobacterium leprae
Epitopos
topic Hanseníase
Áreas endêmicas
Transmissão
Mycobacterium leprae
Epitopos
Mycobacterium leprae
Leprosy
Areas Endemic
Transmission
Pathogen-Specific Epitopes
dc.subject.en.pt_BR.fl_str_mv Mycobacterium leprae
Leprosy
Areas Endemic
Transmission
Pathogen-Specific Epitopes
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2017-01-10T22:07:01Z
dc.date.available.fl_str_mv 2017-01-10T22:07:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARTINS, Márcia V. S. B. et al. Pathogen-Specific Epitopes as Epidemiological Tools for Defining the Magnitude of Mycobacterium leprae transmission in Areas Endemic for Leprosy. Plos Negl Trop Dis., v.6, n.4, e1616, 12p, Apr. 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16792
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0001616
dc.identifier.eissn.none.fl_str_mv 1935-2735
identifier_str_mv MARTINS, Márcia V. S. B. et al. Pathogen-Specific Epitopes as Epidemiological Tools for Defining the Magnitude of Mycobacterium leprae transmission in Areas Endemic for Leprosy. Plos Negl Trop Dis., v.6, n.4, e1616, 12p, Apr. 2012.
1935-2727
10.1371/journal.pntd.0001616
1935-2735
url https://www.arca.fiocruz.br/handle/icict/16792
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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