Characterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/19340 |
Resumo: | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil. |
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Lima-Junior, James DaltroViana-Niero, CristinaOliveira, Daniel V. CondeMachado, Gabriel EsquitiniRabello, Michelle Cristiane da SilvaMartins-Junior, JoaquimMartins, Layla FarageDigiampietri, Luciano AntonioSilva, Aline Maria daSetubal, João CarlosRussell, Daniel A.Jacobs-Sera, DeborahPope, Welkin H.Hatfull, Graham F.Leão, Sylvia Cardoso2017-06-07T18:15:08Z2017-06-07T18:15:08Z2016LIMA-JUNIOR, J. D. et al. Characterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U. BMC Microbiology, v. 16, n. 1, p. 1-15, Dec. 2016.1471-2180https://www.arca.fiocruz.br/handle/icict/1934010.1186/s12866-016-0734-3engCompostagemMicobacteriófagosMycobacterium smegmatisDiversidade de fagoSequenciamento completo do genomaMycobacteriophagesMycobacterium smegmatisCompostingPhage diversityWhole genome sequencingMicobacteriófagos / genéticaMicobacteriófagos / isolamento e purificaçãoMycobacterium / genéticaMycobacterium / virologiaSoloMicrobiologia do SoloCharacterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster Uinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.Universidade Federal de São Paulo. Campus Diadema. Departamento de Ciências Biológicas. São Paulo, SP, Brazil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Universidade Federal de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brazil.Universidade Federal de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brazil.Universidade Federal de São Paulo. Escola de Artes, Ciências e Humanidades. São Paulo, SP, Brazil.Universidade Federal de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brazil.Universidade Federal de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brazil / Virginia Bioinformatics Institute. Blacksburg, VA, USA.University of Pittsburgh. Department of Biological Sciences. Pittsburgh, PA, USA.University of Pittsburgh. Department of Biological Sciences. Pittsburgh, PA, USA.University of Pittsburgh. Department of Biological Sciences. Pittsburgh, PA, USA.University of Pittsburgh. Department of Biological Sciences. Pittsburgh, PA, USA.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.Background: A large collection of sequenced mycobacteriophages capable of infecting a single host strain of Mycobacterium smegmatis shows considerable genomic diversity with dozens of distinctive types (clusters) and extensive variation within those sharing evident nucleotide sequence similarity. Here we profiled the mycobacterial components of a large composting system at the São Paulo zoo. Results: We isolated and sequenced eight mycobacteriophages using Mycobacterium smegmatis mc2 155 as a host. None of these eight phages infected any of mycobacterial strains isolated from the same materials. The phage isolates span considerable genomic diversity, including two phages (Barriga, Nhonho) related to Subcluster A1 phages, two Cluster B phages (Pops, Subcluster B1; Godines, Subcluster B2), three Subcluster F1 phages (Florinda, Girafales, and Quico), and Madruga, a relative of phage Patience with which it constitutes the new Cluster U. Interestingly, the two Subcluster A1 phages and the three Subcluster F1 phages have genomic relationships indicating relatively recent evolution within a geographically isolated niche in the composting system. Conclusions: We predict that composting systems such as those used to obtain these mycobacteriophages will be a rich source for the isolation of additional phages that will expand our view of bacteriophage diversity and evolution.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19340/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL27316672 2016 lim-cha.oa.pdf27316672 2016 lim-cha.oa.pdfapplication/pdf4233364https://www.arca.fiocruz.br/bitstream/icict/19340/2/27316672%202016%20lim-cha.oa.pdfc74fa92e24ad3b01c1e2ddffd0a50fb7MD52TEXT27316672 2016 lim-cha.oa.pdf.txt27316672 2016 lim-cha.oa.pdf.txtExtracted texttext/plain63829https://www.arca.fiocruz.br/bitstream/icict/19340/3/27316672%202016%20lim-cha.oa.pdf.txt1816909b5ee3dc1a254436f25814b072MD53icict/193402019-04-02 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| dc.title.pt_BR.fl_str_mv |
Characterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U |
| title |
Characterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U |
| spellingShingle |
Characterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U Lima-Junior, James Daltro Compostagem Micobacteriófagos Mycobacterium smegmatis Diversidade de fago Sequenciamento completo do genoma Mycobacteriophages Mycobacterium smegmatis Composting Phage diversity Whole genome sequencing Micobacteriófagos / genética Micobacteriófagos / isolamento e purificação Mycobacterium / genética Mycobacterium / virologia Solo Microbiologia do Solo |
| title_short |
Characterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U |
| title_full |
Characterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U |
| title_fullStr |
Characterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U |
| title_full_unstemmed |
Characterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U |
| title_sort |
Characterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U |
| author |
Lima-Junior, James Daltro |
| author_facet |
Lima-Junior, James Daltro Viana-Niero, Cristina Oliveira, Daniel V. Conde Machado, Gabriel Esquitini Rabello, Michelle Cristiane da Silva Martins-Junior, Joaquim Martins, Layla Farage Digiampietri, Luciano Antonio Silva, Aline Maria da Setubal, João Carlos Russell, Daniel A. Jacobs-Sera, Deborah Pope, Welkin H. Hatfull, Graham F. Leão, Sylvia Cardoso |
| author_role |
author |
| author2 |
Viana-Niero, Cristina Oliveira, Daniel V. Conde Machado, Gabriel Esquitini Rabello, Michelle Cristiane da Silva Martins-Junior, Joaquim Martins, Layla Farage Digiampietri, Luciano Antonio Silva, Aline Maria da Setubal, João Carlos Russell, Daniel A. Jacobs-Sera, Deborah Pope, Welkin H. Hatfull, Graham F. Leão, Sylvia Cardoso |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lima-Junior, James Daltro Viana-Niero, Cristina Oliveira, Daniel V. Conde Machado, Gabriel Esquitini Rabello, Michelle Cristiane da Silva Martins-Junior, Joaquim Martins, Layla Farage Digiampietri, Luciano Antonio Silva, Aline Maria da Setubal, João Carlos Russell, Daniel A. Jacobs-Sera, Deborah Pope, Welkin H. Hatfull, Graham F. Leão, Sylvia Cardoso |
| dc.subject.other.none.fl_str_mv |
Compostagem Micobacteriófagos Mycobacterium smegmatis Diversidade de fago Sequenciamento completo do genoma |
| topic |
Compostagem Micobacteriófagos Mycobacterium smegmatis Diversidade de fago Sequenciamento completo do genoma Mycobacteriophages Mycobacterium smegmatis Composting Phage diversity Whole genome sequencing Micobacteriófagos / genética Micobacteriófagos / isolamento e purificação Mycobacterium / genética Mycobacterium / virologia Solo Microbiologia do Solo |
| dc.subject.en.none.fl_str_mv |
Mycobacteriophages Mycobacterium smegmatis Composting Phage diversity Whole genome sequencing |
| dc.subject.decs.none.fl_str_mv |
Micobacteriófagos / genética Micobacteriófagos / isolamento e purificação Mycobacterium / genética Mycobacterium / virologia Solo Microbiologia do Solo |
| description |
Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil. |
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2016 |
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2016 |
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2017-06-07T18:15:08Z |
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2017-06-07T18:15:08Z |
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info:eu-repo/semantics/article |
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LIMA-JUNIOR, J. D. et al. Characterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U. BMC Microbiology, v. 16, n. 1, p. 1-15, Dec. 2016. |
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https://www.arca.fiocruz.br/handle/icict/19340 |
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1471-2180 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1186/s12866-016-0734-3 |
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LIMA-JUNIOR, J. D. et al. Characterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U. BMC Microbiology, v. 16, n. 1, p. 1-15, Dec. 2016. 1471-2180 10.1186/s12866-016-0734-3 |
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