Liver injury and viremia in mice infected with dengue-2 virus
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2005 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/37798 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil. |
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Paes, M. V.Pinhão, A. T.Barreto, D. F.Costa, S. M.Oliveira, M. P.Nogueira, A. C.Takiya, C. M.Farias Filho, J. C.Schatzmayr, H. G.Alves, A. M. B.Barth, Ortud Monika2019-12-10T17:16:13Z2019-12-10T17:16:13Z2005PAES, M. V. et al. Liver injury and viremia in mice infected with dengue-2 virus. Virology, v. 338, p. 236-246, 2005.0042-682https://www.arca.fiocruz.br/handle/icict/3779810.1016/j.virol.2005.04.0421096-0341engElsevierVírus da Dengue tipo 2CamundongosFígadoViremiaHistopatologiaTransaminasesDENV-2MouseLiverViremiaHistopathologyTransaminasesLiver injury and viremia in mice infected with dengue-2 virusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de HIstologia e Embriologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil.The goal of this study was to test the feasibility of BALB/c mice as an experimental model in the study of dengue disease. BALB/c mice were intraperitoneal infected with DENV-2 obtained from a human patient. Histopathological analysis of infected animals revealed liver injury with viral antigens detection. In initial stages, the most prominent lesions were vacuolization and diffuse steatosis in hepatocytes. Serum levels of ALT and AST increased progressively, reaching the highest values 7 days p.i. and decreasing at the 14th day. Since levels of circulating virus were very low, viremia was analyzed in C6/36 cells. Virus presence was detected by ultrastructural analysis, confirmed by RT-PCR assays. Period of viremia was analyzed by flow cytometry with cells incubated with mouse-infected sera collected in different days, revealing peak virus levels at the 7th day p.i. All such data correlate to the development of the disease described in humans.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/37798/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMVPaes_DFBarreto_etal_IOC_2005.pdfMVPaes_DFBarreto_etal_IOC_2005.pdfapplication/pdf1222094https://www.arca.fiocruz.br/bitstream/icict/37798/2/MVPaes_DFBarreto_etal_IOC_2005.pdfb726da9b8eca2343a30c1db08c54f62fMD52TEXTMVPaes_DFBarreto_etal_IOC_2005.pdf.txtMVPaes_DFBarreto_etal_IOC_2005.pdf.txtExtracted texttext/plain42990https://www.arca.fiocruz.br/bitstream/icict/37798/3/MVPaes_DFBarreto_etal_IOC_2005.pdf.txtdb60394bbfafb32ca34f533547f7b1cdMD53icict/377982019-12-11 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| dc.title.pt_BR.fl_str_mv |
Liver injury and viremia in mice infected with dengue-2 virus |
| title |
Liver injury and viremia in mice infected with dengue-2 virus |
| spellingShingle |
Liver injury and viremia in mice infected with dengue-2 virus Paes, M. V. Vírus da Dengue tipo 2 Camundongos Fígado Viremia Histopatologia Transaminases DENV-2 Mouse Liver Viremia Histopathology Transaminases |
| title_short |
Liver injury and viremia in mice infected with dengue-2 virus |
| title_full |
Liver injury and viremia in mice infected with dengue-2 virus |
| title_fullStr |
Liver injury and viremia in mice infected with dengue-2 virus |
| title_full_unstemmed |
Liver injury and viremia in mice infected with dengue-2 virus |
| title_sort |
Liver injury and viremia in mice infected with dengue-2 virus |
| author |
Paes, M. V. |
| author_facet |
Paes, M. V. Pinhão, A. T. Barreto, D. F. Costa, S. M. Oliveira, M. P. Nogueira, A. C. Takiya, C. M. Farias Filho, J. C. Schatzmayr, H. G. Alves, A. M. B. Barth, Ortud Monika |
| author_role |
author |
| author2 |
Pinhão, A. T. Barreto, D. F. Costa, S. M. Oliveira, M. P. Nogueira, A. C. Takiya, C. M. Farias Filho, J. C. Schatzmayr, H. G. Alves, A. M. B. Barth, Ortud Monika |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Paes, M. V. Pinhão, A. T. Barreto, D. F. Costa, S. M. Oliveira, M. P. Nogueira, A. C. Takiya, C. M. Farias Filho, J. C. Schatzmayr, H. G. Alves, A. M. B. Barth, Ortud Monika |
| dc.subject.other.pt_BR.fl_str_mv |
Vírus da Dengue tipo 2 Camundongos Fígado Viremia Histopatologia Transaminases |
| topic |
Vírus da Dengue tipo 2 Camundongos Fígado Viremia Histopatologia Transaminases DENV-2 Mouse Liver Viremia Histopathology Transaminases |
| dc.subject.en.pt_BR.fl_str_mv |
DENV-2 Mouse Liver Viremia Histopathology Transaminases |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil. |
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2005 |
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2005 |
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2019-12-10T17:16:13Z |
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2019-12-10T17:16:13Z |
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PAES, M. V. et al. Liver injury and viremia in mice infected with dengue-2 virus. Virology, v. 338, p. 236-246, 2005. |
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https://www.arca.fiocruz.br/handle/icict/37798 |
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0042-682 |
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10.1016/j.virol.2005.04.042 |
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1096-0341 |
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PAES, M. V. et al. Liver injury and viremia in mice infected with dengue-2 virus. Virology, v. 338, p. 236-246, 2005. 0042-682 10.1016/j.virol.2005.04.042 1096-0341 |
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