Transplantation of adipose tissue mesenchymal stem cells in experimental chronic chagasic cardiopathy.

Detalhes bibliográficos
Autor(a) principal: Larocca, Ticiana Ferreira
Data de Publicação: 2013
Outros Autores: Freitas Souza, Bruno Solano de, Silva, Cristina Aragão, Kaneto, Carla Martins, Alcantara, Adriano Costa de, Azevedo, Carine Machado, Castro, Murilo Fagundes de, Macambira, Simone Garcia, Soares, Milena Botelho Pereira, Santos, Ricardo Ribeiro dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.5935/abc.20130058
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8566
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil
id CRUZ_050dc21406d3d62883e3c1c549285c01
oai_identifier_str oai:www.arca.fiocruz.br:icict/8566
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Larocca, Ticiana FerreiraFreitas Souza, Bruno Solano deSilva, Cristina AragãoKaneto, Carla MartinsAlcantara, Adriano Costa deAzevedo, Carine MachadoCastro, Murilo Fagundes deMacambira, Simone GarciaSoares, Milena Botelho PereiraSantos, Ricardo Ribeiro dos2014-10-09T14:26:11Z2014-10-09T14:26:11Z2013LAROCCA, T. F. et al. Transplantation of adipose tissue mesenchymal stem cells in experimental chronic chagasic cardiopathy. Arquivos Brasileiros de Cardiologia, v. 100, n. 5, p. 460-468, 2013.1678-4170https://www.arca.fiocruz.br/handle/icict/856610.5935/abc.20130058engSociedade Brasileira de CardiologiaTransplantation of adipose tissue mesenchymal stem cells in experimental chronic chagasic cardiopathy.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilHospital São Rafael. Salvador, BA, BrasilHospital São Rafael. Salvador, BA, BrasilHospital São Rafael. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilBACKGROUND: Chagas disease, caused by the protozoan Trypanosoma cruzi, is a major cause of heart failure in Latin America. Tissue therapy has been investigated as a possible therapeutic option for patients with cardiovascular disease. OBJECTIVE: This study evaluated the effects of therapy with mesenchymal stem cells in an experimental model of chronic Chagasic cardiomyopathy. METHODS: C57BL/6 mice were infected with 1000 trypomastigotes from the Colombian strain of T. cruzi and, after six months of infection, were treated with mesenchymal human stem cells from adipose tissue (STAT) or with Dulbecco/Vogt modified Eagle's minimal essential medium - DMEM (control). The treated group received two intraperitoneal injections of STAT (1x10(6) cells/dose), with a month interval between the two doses. Before and after the first and second months of treatment, the chagasic and normal control animals underwent cardiopulmonary exercise testing and electrocardiography. All animals were sacrificed under anesthesia after two months of treatment for histopathological analysis of the heart. RESULTS: No improvement was observed in arrhythmias and cardiovascular function in the group of animals treated with STAT; however, sections of mice hearts in this group revealed a significant reduction in the number of inflammatory cells (p<0.0001) and areas of fibrosis (p<0.01) in comparison with chagasic animals treated with DMEM. CONCLUSION: Thus, it is concluded that administration of intraperitoneal STAT can reduce inflammation and fibrosis in the heart of mice chronically infected with T. cruzi; however, there were no effects on the cardiac function two months after transplantation.Chagas Cardiomyopathy / therapyStem CellsTissue TherapyAdipose TissueTecido Adiposo/citologiaCardiomiopatia Chagásica/cirurgiaTransplante de Células-Tronco MesenquimaisTrypanosoma cruziAnálise de VariânciaAnimaisArritmias Cardíacas/metabolismoCardiomiopatia Chagásica/patologiaCardiomiopatia Chagásica/fisiopatologiaModelos Animais de DoençasFibroseInflamação/metabolismoInjeções IntraperitoneaisCamundongosCamundongos Endogâmicos C57BLCondicionamento Físico Animal/métodosDistribuição Aleatóriainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8566/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALLarocca T F Transplantation of....pdfLarocca T F Transplantation of....pdfapplication/pdf1434012https://www.arca.fiocruz.br/bitstream/icict/8566/2/Larocca%20T%20F%20Transplantation%20of....pdfda88203f57a22ad3e82c312d3666d4b0MD52TEXTLarocca T F Transplantation of....pdf.txtLarocca T F Transplantation of....pdf.txtExtracted texttext/plain34396https://www.arca.fiocruz.br/bitstream/icict/8566/3/Larocca%20T%20F%20Transplantation%20of....pdf.txt03df259767cf93fa02e22ff9f1e74f3aMD53icict/85662023-03-15 14:33:24.536oai:www.arca.fiocruz.br:icict/8566TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:24Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Transplantation of adipose tissue mesenchymal stem cells in experimental chronic chagasic cardiopathy.
title Transplantation of adipose tissue mesenchymal stem cells in experimental chronic chagasic cardiopathy.
spellingShingle Transplantation of adipose tissue mesenchymal stem cells in experimental chronic chagasic cardiopathy.
Larocca, Ticiana Ferreira
Chagas Cardiomyopathy / therapy
Stem Cells
Tissue Therapy
Adipose Tissue
Tecido Adiposo/citologia
Cardiomiopatia Chagásica/cirurgia
Transplante de Células-Tronco Mesenquimais
Trypanosoma cruzi
Análise de Variância
Animais
Arritmias Cardíacas/metabolismo
Cardiomiopatia Chagásica/patologia
Cardiomiopatia Chagásica/fisiopatologia
Modelos Animais de Doenças
Fibrose
Inflamação/metabolismo
Injeções Intraperitoneais
Camundongos
Camundongos Endogâmicos C57BL
Condicionamento Físico Animal/métodos
Distribuição Aleatória
title_short Transplantation of adipose tissue mesenchymal stem cells in experimental chronic chagasic cardiopathy.
title_full Transplantation of adipose tissue mesenchymal stem cells in experimental chronic chagasic cardiopathy.
title_fullStr Transplantation of adipose tissue mesenchymal stem cells in experimental chronic chagasic cardiopathy.
title_full_unstemmed Transplantation of adipose tissue mesenchymal stem cells in experimental chronic chagasic cardiopathy.
title_sort Transplantation of adipose tissue mesenchymal stem cells in experimental chronic chagasic cardiopathy.
author Larocca, Ticiana Ferreira
author_facet Larocca, Ticiana Ferreira
Freitas Souza, Bruno Solano de
Silva, Cristina Aragão
Kaneto, Carla Martins
Alcantara, Adriano Costa de
Azevedo, Carine Machado
Castro, Murilo Fagundes de
Macambira, Simone Garcia
Soares, Milena Botelho Pereira
Santos, Ricardo Ribeiro dos
author_role author
author2 Freitas Souza, Bruno Solano de
Silva, Cristina Aragão
Kaneto, Carla Martins
Alcantara, Adriano Costa de
Azevedo, Carine Machado
Castro, Murilo Fagundes de
Macambira, Simone Garcia
Soares, Milena Botelho Pereira
Santos, Ricardo Ribeiro dos
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Larocca, Ticiana Ferreira
Freitas Souza, Bruno Solano de
Silva, Cristina Aragão
Kaneto, Carla Martins
Alcantara, Adriano Costa de
Azevedo, Carine Machado
Castro, Murilo Fagundes de
Macambira, Simone Garcia
Soares, Milena Botelho Pereira
Santos, Ricardo Ribeiro dos
dc.subject.en.pt_BR.fl_str_mv Chagas Cardiomyopathy / therapy
Stem Cells
Tissue Therapy
Adipose Tissue
topic Chagas Cardiomyopathy / therapy
Stem Cells
Tissue Therapy
Adipose Tissue
Tecido Adiposo/citologia
Cardiomiopatia Chagásica/cirurgia
Transplante de Células-Tronco Mesenquimais
Trypanosoma cruzi
Análise de Variância
Animais
Arritmias Cardíacas/metabolismo
Cardiomiopatia Chagásica/patologia
Cardiomiopatia Chagásica/fisiopatologia
Modelos Animais de Doenças
Fibrose
Inflamação/metabolismo
Injeções Intraperitoneais
Camundongos
Camundongos Endogâmicos C57BL
Condicionamento Físico Animal/métodos
Distribuição Aleatória
dc.subject.decs.pt_BR.fl_str_mv Tecido Adiposo/citologia
Cardiomiopatia Chagásica/cirurgia
Transplante de Células-Tronco Mesenquimais
Trypanosoma cruzi
Análise de Variância
Animais
Arritmias Cardíacas/metabolismo
Cardiomiopatia Chagásica/patologia
Cardiomiopatia Chagásica/fisiopatologia
Modelos Animais de Doenças
Fibrose
Inflamação/metabolismo
Injeções Intraperitoneais
Camundongos
Camundongos Endogâmicos C57BL
Condicionamento Físico Animal/métodos
Distribuição Aleatória
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-10-09T14:26:11Z
dc.date.available.fl_str_mv 2014-10-09T14:26:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LAROCCA, T. F. et al. Transplantation of adipose tissue mesenchymal stem cells in experimental chronic chagasic cardiopathy. Arquivos Brasileiros de Cardiologia, v. 100, n. 5, p. 460-468, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8566
dc.identifier.issn.none.fl_str_mv 1678-4170
dc.identifier.doi.none.fl_str_mv 10.5935/abc.20130058
identifier_str_mv LAROCCA, T. F. et al. Transplantation of adipose tissue mesenchymal stem cells in experimental chronic chagasic cardiopathy. Arquivos Brasileiros de Cardiologia, v. 100, n. 5, p. 460-468, 2013.
1678-4170
10.5935/abc.20130058
url https://www.arca.fiocruz.br/handle/icict/8566
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Cardiologia
publisher.none.fl_str_mv Sociedade Brasileira de Cardiologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/8566/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/8566/2/Larocca%20T%20F%20Transplantation%20of....pdf
https://www.arca.fiocruz.br/bitstream/icict/8566/3/Larocca%20T%20F%20Transplantation%20of....pdf.txt
bitstream.checksum.fl_str_mv 7d48279ffeed55da8dfe2f8e81f3b81f
da88203f57a22ad3e82c312d3666d4b0
03df259767cf93fa02e22ff9f1e74f3a
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1822792112332079104

Registros relacionados