Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23480 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil. |
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Moreira, Otacilio C.Verly, ThaianeAraujo, Paula FinamoreGomes, Suzete A. O.Lopes, Catarina M.Sousa, Danielle M. deAzevedo, Lívia R.Mota, Fabio .F. dad`Avila-Levy, Claudia M.Santos-Mallet, Jacenir R.Britto, Constança2017-11-30T16:07:33Z2017-11-30T16:07:33Z2017MOREIRA, Otacílio C.; et al. Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors. Parasites & Vectors, v.10:404, 14p, 2017.1756-3305https://www.arca.fiocruz.br/handle/icict/2348010.1186/s13071-017-2343-xengBioMed CentralTrypanosoma cruziReação em Cadeia da Polimerase MultiplexReação em Cadeia da Polimerase em Tempo RealTriatominaeCarga ParasitáriaInfecção naturalTrypanosoma cruziMultiplex PCRReal-time qPCRTriatominesNatural infectionParasite loadDevelopment of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectorsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.Universidade Federal Fluminense. Laboratório de Biodiversidade de Parasitas e Vetores. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Vigilância Entomológica de Diptera e Hemiptera. Rio de janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Vigilância Entomológica de Diptera e Hemiptera. Rio de janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Estudos Integrados em Protozoologia. Rio de Janeiro, RJ. Brasil .Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Vigilância Entomológica de Diptera e Hemiptera. Rio de janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.Chagas disease is a complex anthropozoonosis with distinct domestic and sylvatic mammal species acting as potential reservoirs. The diversity of vector species and their habitats are among the factors that hinder the control of the disease. Control programs periodically monitor the prevalence of T. cruzi infection in insect bugs through microscopical observation of diluted feces. However, microscopy presents limited sensitivity in samples with low parasite numbers, difficulties in examining all evolutionary stages of the insect and may in turn be limited to differentiate T. cruzi from other morphologically similar trypanosomatids. Here, we report two highly sensitive and accurate methodologies to infer T. cruzi infection rates and to quantify parasite load in the gut of field-collected triatomines.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23480/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALotacilio_moreira_etal_IOC_2017.pdfotacilio_moreira_etal_IOC_2017.pdfapplication/pdf2626749https://www.arca.fiocruz.br/bitstream/icict/23480/2/otacilio_moreira_etal_IOC_2017.pdfc4d8e1d885f6e699789d9818f7b79e3dMD52TEXTotacilio_moreira_etal_IOC_2017.pdf.txtotacilio_moreira_etal_IOC_2017.pdf.txtExtracted texttext/plain67996https://www.arca.fiocruz.br/bitstream/icict/23480/3/otacilio_moreira_etal_IOC_2017.pdf.txtf15606bc3a0b2f8a7ffe71dc03365c5bMD53icict/234802022-06-24 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| dc.title.pt_BR.fl_str_mv |
Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors |
| title |
Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors |
| spellingShingle |
Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors Moreira, Otacilio C. Trypanosoma cruzi Reação em Cadeia da Polimerase Multiplex Reação em Cadeia da Polimerase em Tempo Real Triatominae Carga Parasitária Infecção natural Trypanosoma cruzi Multiplex PCR Real-time qPCR Triatomines Natural infection Parasite load |
| title_short |
Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors |
| title_full |
Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors |
| title_fullStr |
Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors |
| title_full_unstemmed |
Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors |
| title_sort |
Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors |
| author |
Moreira, Otacilio C. |
| author_facet |
Moreira, Otacilio C. Verly, Thaiane Araujo, Paula Finamore Gomes, Suzete A. O. Lopes, Catarina M. Sousa, Danielle M. de Azevedo, Lívia R. Mota, Fabio .F. da d`Avila-Levy, Claudia M. Santos-Mallet, Jacenir R. Britto, Constança |
| author_role |
author |
| author2 |
Verly, Thaiane Araujo, Paula Finamore Gomes, Suzete A. O. Lopes, Catarina M. Sousa, Danielle M. de Azevedo, Lívia R. Mota, Fabio .F. da d`Avila-Levy, Claudia M. Santos-Mallet, Jacenir R. Britto, Constança |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Moreira, Otacilio C. Verly, Thaiane Araujo, Paula Finamore Gomes, Suzete A. O. Lopes, Catarina M. Sousa, Danielle M. de Azevedo, Lívia R. Mota, Fabio .F. da d`Avila-Levy, Claudia M. Santos-Mallet, Jacenir R. Britto, Constança |
| dc.subject.other.pt_BR.fl_str_mv |
Trypanosoma cruzi Reação em Cadeia da Polimerase Multiplex Reação em Cadeia da Polimerase em Tempo Real Triatominae Carga Parasitária Infecção natural |
| topic |
Trypanosoma cruzi Reação em Cadeia da Polimerase Multiplex Reação em Cadeia da Polimerase em Tempo Real Triatominae Carga Parasitária Infecção natural Trypanosoma cruzi Multiplex PCR Real-time qPCR Triatomines Natural infection Parasite load |
| dc.subject.en.pt_BR.fl_str_mv |
Trypanosoma cruzi Multiplex PCR Real-time qPCR Triatomines Natural infection Parasite load |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil. |
| publishDate |
2017 |
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2017-11-30T16:07:33Z |
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2017-11-30T16:07:33Z |
| dc.date.issued.fl_str_mv |
2017 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
MOREIRA, Otacílio C.; et al. Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors. Parasites & Vectors, v.10:404, 14p, 2017. |
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https://www.arca.fiocruz.br/handle/icict/23480 |
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1756-3305 |
| dc.identifier.doi.none.fl_str_mv |
10.1186/s13071-017-2343-x |
| identifier_str_mv |
MOREIRA, Otacílio C.; et al. Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors. Parasites & Vectors, v.10:404, 14p, 2017. 1756-3305 10.1186/s13071-017-2343-x |
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https://www.arca.fiocruz.br/handle/icict/23480 |
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eng |
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BioMed Central |
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BioMed Central |
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