Infection with Leishmania amazonensis upregulates purinergic receptor expression and induces host-cell susceptibility to UTP-mediated apoptosis

Detalhes bibliográficos
Autor(a) principal: Silva, Camila Marques da
Data de Publicação: 2011
Outros Autores: Chaves, Mariana M., Chaves, Suzana Passos, Figliuolo, Vanessa Ribeiro, Fernandes, José Roberto Meyer, Corte-Real, Suzana, Lameu, Claudiana, Ulrich, Henning, Ojcius, David M., Bergmann, Bartira Rossi, Silva, Robson Coutinho
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16158
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofisiologia. Rio de Janeiro, RJ, Brasil.
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spelling Silva, Camila Marques daChaves, Mariana M.Chaves, Suzana PassosFigliuolo, Vanessa RibeiroFernandes, José Roberto MeyerCorte-Real, SuzanaLameu, ClaudianaUlrich, HenningOjcius, David M.Bergmann, Bartira RossiSilva, Robson Coutinho2016-10-08T17:40:13Z2016-10-08T17:40:13Z2011SILVA, Camila Marques da; et al. Infection with Leishmania amazonensis upregulates purinergic receptor expression and induces host-cell susceptibility to UTP-mediated apoptosis. Cellular Microbiology, v.13, n;9, p.1410-1428, 2011.1462-5814https://www.arca.fiocruz.br/handle/icict/1615810.1111/j.1462-5822.2011.01630.x1462-5822engWiley Online LibraryLeishmania amazonensisMacrófagosSusceptibilidade das células de acolhimentoApoptoseReceptor purinérgicoLeishmania amazonensismacrophagesapoptosishost-cell susceptibilitypurinergic receptorInfection with Leishmania amazonensis upregulates purinergic receptor expression and induces host-cell susceptibility to UTP-mediated apoptosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofisiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofisiologia. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofisiologia. Laboratório de Imunofarmoclogia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofisiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Instituto Butantan. Centro de Toxinologia Aplicada - CAT-CEPID. São Paulo, SP, Brasil / Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil.Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil.University of California. Research Institute and School of Natural Sciences. Merdec, CA, USA.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Rio de janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofisiologia. Rio de Janeiro, RJ, Brasil.Nucleotides are released into the extracellular milieu from infected cells and cells at inflammatory sites. The extracellular nucleotides bind to specific purinergic (P2) receptors and thereby induce a variety of cellular responses including anti-parasitic effects. Here we investigated whether extracellular nucleotides affect leishmanial infection in macrophages, and found that UTP reduces strongly the parasite load in peritoneal macrophages. Ultrastructural analysis of infected cells revealed that UTP induced morphological damage in the intracellular parasites. Uridine nucleotides also induced dose-dependent apoptosis of macrophages and production of ROI and RNI only in infected macrophages. The intracellular calcium measurements of infected cells showed that the response to UTP, but not UDP, increased the sensitivity and amplitude of cytosolic Ca(2+) changes. Infection of macrophages with Leishmania upregulated the expression of P2Y(2) and P2Y(4) receptor mRNA. The data suggest indirectly that Leishmania amazonensis infection induces modulation and heteromerization of P2Y receptors on macrophages. Thus UTP modulates the host response against L. amazonensis infection. UTP and UTP homologues should therefore be considered as novel components of therapeutic strategies against cutaneous leishmaniasis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16158/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALsuzana_cortereal_etal_IOC_2011.df.pdfsuzana_cortereal_etal_IOC_2011.df.pdfapplication/pdf1424487https://www.arca.fiocruz.br/bitstream/icict/16158/2/suzana_cortereal_etal_IOC_2011.df.pdfa4c4e0a455733877cd736ba3f87cf022MD52TEXTsuzana_cortereal_etal_IOC_2011.df.pdf.txtsuzana_cortereal_etal_IOC_2011.df.pdf.txtExtracted texttext/plain74089https://www.arca.fiocruz.br/bitstream/icict/16158/3/suzana_cortereal_etal_IOC_2011.df.pdf.txtfe67c89db18b83b516710549ecfbecfeMD53icict/161582018-04-02 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dc.title.pt_BR.fl_str_mv Infection with Leishmania amazonensis upregulates purinergic receptor expression and induces host-cell susceptibility to UTP-mediated apoptosis
title Infection with Leishmania amazonensis upregulates purinergic receptor expression and induces host-cell susceptibility to UTP-mediated apoptosis
spellingShingle Infection with Leishmania amazonensis upregulates purinergic receptor expression and induces host-cell susceptibility to UTP-mediated apoptosis
Silva, Camila Marques da
Leishmania amazonensis
Macrófagos
Susceptibilidade das células de acolhimento
Apoptose
Receptor purinérgico
Leishmania amazonensis
macrophages
apoptosis
host-cell susceptibility
purinergic receptor
title_short Infection with Leishmania amazonensis upregulates purinergic receptor expression and induces host-cell susceptibility to UTP-mediated apoptosis
title_full Infection with Leishmania amazonensis upregulates purinergic receptor expression and induces host-cell susceptibility to UTP-mediated apoptosis
title_fullStr Infection with Leishmania amazonensis upregulates purinergic receptor expression and induces host-cell susceptibility to UTP-mediated apoptosis
title_full_unstemmed Infection with Leishmania amazonensis upregulates purinergic receptor expression and induces host-cell susceptibility to UTP-mediated apoptosis
title_sort Infection with Leishmania amazonensis upregulates purinergic receptor expression and induces host-cell susceptibility to UTP-mediated apoptosis
author Silva, Camila Marques da
author_facet Silva, Camila Marques da
Chaves, Mariana M.
Chaves, Suzana Passos
Figliuolo, Vanessa Ribeiro
Fernandes, José Roberto Meyer
Corte-Real, Suzana
Lameu, Claudiana
Ulrich, Henning
Ojcius, David M.
Bergmann, Bartira Rossi
Silva, Robson Coutinho
author_role author
author2 Chaves, Mariana M.
Chaves, Suzana Passos
Figliuolo, Vanessa Ribeiro
Fernandes, José Roberto Meyer
Corte-Real, Suzana
Lameu, Claudiana
Ulrich, Henning
Ojcius, David M.
Bergmann, Bartira Rossi
Silva, Robson Coutinho
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Camila Marques da
Chaves, Mariana M.
Chaves, Suzana Passos
Figliuolo, Vanessa Ribeiro
Fernandes, José Roberto Meyer
Corte-Real, Suzana
Lameu, Claudiana
Ulrich, Henning
Ojcius, David M.
Bergmann, Bartira Rossi
Silva, Robson Coutinho
dc.subject.other.pt_BR.fl_str_mv Leishmania amazonensis
Macrófagos
Susceptibilidade das células de acolhimento
Apoptose
Receptor purinérgico
topic Leishmania amazonensis
Macrófagos
Susceptibilidade das células de acolhimento
Apoptose
Receptor purinérgico
Leishmania amazonensis
macrophages
apoptosis
host-cell susceptibility
purinergic receptor
dc.subject.en.pt_BR.fl_str_mv Leishmania amazonensis
macrophages
apoptosis
host-cell susceptibility
purinergic receptor
description Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofisiologia. Rio de Janeiro, RJ, Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2016-10-08T17:40:13Z
dc.date.available.fl_str_mv 2016-10-08T17:40:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Camila Marques da; et al. Infection with Leishmania amazonensis upregulates purinergic receptor expression and induces host-cell susceptibility to UTP-mediated apoptosis. Cellular Microbiology, v.13, n;9, p.1410-1428, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16158
dc.identifier.issn.pt_BR.fl_str_mv 1462-5814
dc.identifier.doi.none.fl_str_mv 10.1111/j.1462-5822.2011.01630.x
dc.identifier.eissn.none.fl_str_mv 1462-5822
identifier_str_mv SILVA, Camila Marques da; et al. Infection with Leishmania amazonensis upregulates purinergic receptor expression and induces host-cell susceptibility to UTP-mediated apoptosis. Cellular Microbiology, v.13, n;9, p.1410-1428, 2011.
1462-5814
10.1111/j.1462-5822.2011.01630.x
1462-5822
url https://www.arca.fiocruz.br/handle/icict/16158
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley Online Library
publisher.none.fl_str_mv Wiley Online Library
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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