Silencing of amygdala circuits during sepsis prevents the development of anxiety-related behaviours

Detalhes bibliográficos
Autor(a) principal: Bourhy, Lena
Data de Publicação: 2022
Outros Autores: Mazeraud, Aurélien, Costa, Luis H. A., Levy, Jarod, Rei, Damien, Hecquet, Estéban, Gabanyi, Ilana, Bozza, Fernando A., Chrétien, Fabrice, Lledo, Pierre-Marie, Sharshar, Tarek, Lepousez, Gabriel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/55222
Resumo: Institut Pasteur. Université Paris Cité. Laboratory for Experimental Neuropathology. Paris, France / Institut Pasteur. Université Paris Cité. CNRS UMR 3571. Perception and Memory Unit. Paris, France / Université Paris Cité. Collège doctoral. Paris, France.
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spelling Bourhy, LenaMazeraud, AurélienCosta, Luis H. A.Levy, JarodRei, DamienHecquet, EstébanGabanyi, IlanaBozza, Fernando A.Chrétien, FabriceLledo, Pierre-MarieSharshar, TarekLepousez, Gabriel2022-10-20T22:54:48Z2022-10-20T22:54:48Z2022BOURHY, Lena et al. Silencing of amygdala circuits during sepsis prevents the development of anxiety-related behaviours. Brain: a Journal of Neurology, v. 145, n. 4, p. 1391-1409, 2022.0006-8950https://www.arca.fiocruz.br/handle/icict/5522210.1093/brain/awab475engOxfordSilencing of amygdala circuits during sepsis prevents the development of anxiety-related behavioursinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstitut Pasteur. Université Paris Cité. Laboratory for Experimental Neuropathology. Paris, France / Institut Pasteur. Université Paris Cité. CNRS UMR 3571. Perception and Memory Unit. Paris, France / Université Paris Cité. Collège doctoral. Paris, France.Institut Pasteur. Université Paris Cité. Laboratory for Experimental Neuropathology. Paris, France / Université Paris Cité. Collège doctoral. Paris, France / GHU Paris Psychiatrie Neurosciences. Service hospitalo-universitaire de Neuro-anesthésie réanimation. Paris, France.Institut Pasteur. Université Paris Cité. Laboratory for Experimental Neuropathology. Paris, France / University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Basic and Oral Biology. Ribeirão Preto, SP, Brazil.Institut Pasteur. Université Paris Cité. CNRS UMR 3571. Perception and Memory Unit. Paris, France.Institut Pasteur. Université Paris Cité. CNRS UMR 3571. Perception and Memory Unit. Paris, France.Institut Pasteur. Université Paris Cité. CNRS UMR 3571. Perception and Memory Unit. Paris, France.Institut Pasteur. Université Paris Cité. CNRS UMR 3571. Perception and Memory Unit. Paris, France / Institut Pasteur. Université Paris Cité. Microenvironment and Immunity Unit. Paris, France.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil / D'Or Institute for Research and Education. Rio de Janeiro, RJ, Brazil.Institut Pasteur. Université Paris Cité. Laboratory for Experimental Neuropathology. Paris, France / GHU Paris Psychiatrie Neurosciences. Service hospitalo-universitaire de Neuropathologie. Paris, France.Institut Pasteur. Université Paris Cité. CNRS UMR 3571. Perception and Memory Unit. Paris, France.HU Paris Psychiatrie Neurosciences. Service hospitalo-universitaire de Neuro-anesthésie réanimation. Paris, France / Institut de Psychiatrie et Neurosciences de Paris. INSERM UMR 1266. Paris, France.Institut Pasteur. Université Paris Cité. CNRS UMR 3571. Perception and Memory Unit. Paris, France.Sepsis is a life-threatening condition induced by a deregulated host response to severe infection. Post-sepsis syndrome includes long-term psychiatric disorders, such as persistent anxiety and post-traumatic stress disorder, whose neurobiological mechanisms remain unknown. Using a reference mouse model of sepsis, we showed that mice that recovered from sepsis further developed anxiety-related behaviours associated with an exaggerated fear memory. In the brain, sepsis induced an acute pathological activation of a specific neuronal population of the central nucleus of the amygdala, which projects to the ventral bed nucleus of the stria terminalis. Using viral-genetic circuit tracing and in vivo calcium imaging, we observed that sepsis induced persistent changes in the connectivity matrix and in the responsiveness of these central amygdala neurons projecting to the ventral bed nucleus of the stria terminalis. The transient and targeted silencing of this subpopulation only during the acute phase of sepsis with a viral pharmacogenetic approach, or with the anti-epileptic and neuroprotective drug levetiracetam, prevented the subsequent development of anxiety-related behaviours. Specific inhibition of brain anxiety and fear circuits during the sepsis acute phase constitutes a preventive approach to preclude the post-infection psychiatric outcomes.Cecal ligation and punctureFear conditioningNeuro-immune interactionsNeuroinflammationOptogeneticinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/55222/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSilencing_Fernando_Bozza_etal_INI_2022.pdfSilencing_Fernando_Bozza_etal_INI_2022.pdfapplication/pdf1961639https://www.arca.fiocruz.br/bitstream/icict/55222/2/Silencing_Fernando_Bozza_etal_INI_2022.pdf673f32a016851966bf2cf8a85e86f808MD52icict/552222023-06-02 21:04:15.674oai:www.arca.fiocruz.br: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ório 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dc.title.en_US.fl_str_mv Silencing of amygdala circuits during sepsis prevents the development of anxiety-related behaviours
title Silencing of amygdala circuits during sepsis prevents the development of anxiety-related behaviours
spellingShingle Silencing of amygdala circuits during sepsis prevents the development of anxiety-related behaviours
Bourhy, Lena
Cecal ligation and puncture
Fear conditioning
Neuro-immune interactions
Neuroinflammation
Optogenetic
title_short Silencing of amygdala circuits during sepsis prevents the development of anxiety-related behaviours
title_full Silencing of amygdala circuits during sepsis prevents the development of anxiety-related behaviours
title_fullStr Silencing of amygdala circuits during sepsis prevents the development of anxiety-related behaviours
title_full_unstemmed Silencing of amygdala circuits during sepsis prevents the development of anxiety-related behaviours
title_sort Silencing of amygdala circuits during sepsis prevents the development of anxiety-related behaviours
author Bourhy, Lena
author_facet Bourhy, Lena
Mazeraud, Aurélien
Costa, Luis H. A.
Levy, Jarod
Rei, Damien
Hecquet, Estéban
Gabanyi, Ilana
Bozza, Fernando A.
Chrétien, Fabrice
Lledo, Pierre-Marie
Sharshar, Tarek
Lepousez, Gabriel
author_role author
author2 Mazeraud, Aurélien
Costa, Luis H. A.
Levy, Jarod
Rei, Damien
Hecquet, Estéban
Gabanyi, Ilana
Bozza, Fernando A.
Chrétien, Fabrice
Lledo, Pierre-Marie
Sharshar, Tarek
Lepousez, Gabriel
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bourhy, Lena
Mazeraud, Aurélien
Costa, Luis H. A.
Levy, Jarod
Rei, Damien
Hecquet, Estéban
Gabanyi, Ilana
Bozza, Fernando A.
Chrétien, Fabrice
Lledo, Pierre-Marie
Sharshar, Tarek
Lepousez, Gabriel
dc.subject.en.en_US.fl_str_mv Cecal ligation and puncture
Fear conditioning
Neuro-immune interactions
Neuroinflammation
Optogenetic
topic Cecal ligation and puncture
Fear conditioning
Neuro-immune interactions
Neuroinflammation
Optogenetic
description Institut Pasteur. Université Paris Cité. Laboratory for Experimental Neuropathology. Paris, France / Institut Pasteur. Université Paris Cité. CNRS UMR 3571. Perception and Memory Unit. Paris, France / Université Paris Cité. Collège doctoral. Paris, France.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-10-20T22:54:48Z
dc.date.available.fl_str_mv 2022-10-20T22:54:48Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BOURHY, Lena et al. Silencing of amygdala circuits during sepsis prevents the development of anxiety-related behaviours. Brain: a Journal of Neurology, v. 145, n. 4, p. 1391-1409, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/55222
dc.identifier.issn.en_US.fl_str_mv 0006-8950
dc.identifier.doi.none.fl_str_mv 10.1093/brain/awab475
identifier_str_mv BOURHY, Lena et al. Silencing of amygdala circuits during sepsis prevents the development of anxiety-related behaviours. Brain: a Journal of Neurology, v. 145, n. 4, p. 1391-1409, 2022.
0006-8950
10.1093/brain/awab475
url https://www.arca.fiocruz.br/handle/icict/55222
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford
publisher.none.fl_str_mv Oxford
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/55222/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/55222/2/Silencing_Fernando_Bozza_etal_INI_2022.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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