Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Costa, Sidnei Ferro
Data de Publicação: 2020
Outros Autores: Gomes, Vinícius Oliveira, Maciel, Marilene Oliveira dos Santos, Melo, Larissa Martins, Venturin, Gabriela Lovizutto, Bragato, Jaqueline Poleto, Rebech, Gabriela Torres, Santos, Catiule de Oliveira, Oliveira, Bárbara Maria Nascimento de, Oliveira, Geraldo Gileno de Sá, Lima, Valéria Marçal Felix de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/40815
Resumo: São Paulo Research Foundation (FAPESP), process #2017/10906-8
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spelling Costa, Sidnei FerroGomes, Vinícius OliveiraMaciel, Marilene Oliveira dos SantosMelo, Larissa MartinsVenturin, Gabriela LovizuttoBragato, Jaqueline PoletoRebech, Gabriela TorresSantos, Catiule de OliveiraOliveira, Bárbara Maria Nascimento deOliveira, Geraldo Gileno de SáLima, Valéria Marçal Felix de2020-04-17T12:58:38Z2020-04-17T12:58:38Z2020COSTA, Sidnei Ferro et al. Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis. PLoS Neglected Tropical Diseases, p. 1-21, 21 Jan. 2020.1935-2727https://www.arca.fiocruz.br/handle/icict/4081510.1371/journal.pntd.00080211935-2735São Paulo Research Foundation (FAPESP), process #2017/10906-8National Council for Scientific and Technological Development (CNPq), process #400063/2016-6CNPq/INCT-DT, process #573839/2008-5CNPq-PROEP, process #400913/2013-5Coordination for the Improvement of Higher Education Personnel (CAPES) - Finance Code 001Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia Estrutural e Molecular. Salvador, BA, Brasil.Universidade Estadual Paulista "Júlio de Mesquita Filho". Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brasil.Domestic dogs are the main reservoir of Leishmania infantum, a causative agent of visceral leishmaniasis (VL). The number of human disease cases is associated with the rate of canine infection. Currently available drugs are not efficient at treating canine leishmaniasis (CanL) and months after the treatment most dogs show disease relapse, therefore the development of new drugs or new therapeutic strategies should be sought. In CanL, dogs lack the ability to mount a specific cellular immune response suitable for combating the parasite and manipulation of cytokine signaling pathway has the potential to form part of effective immunotherapeutic methods. In this study, recombinant canine cytokines (rcaIL-12, rcaIL-2, rcaIL-15 and rcaIL-7) and soluble receptor IL-10R1 (rcasIL-10R1), with antagonistic activity, were evaluated for the first time in combination (rcaIL-12/rcaIL-2, rcaIL-12/rcaIL-15, rcaIL-12/rcasIL-10R1, rcaIL-15/rcaIL-7) or alone (rcasIL-10R1) to evaluate their immunomodulatory capacity in peripheral blood mononuclear cells (PBMCs) from dogs with leishmaniasis. All the combinations of recombinant proteins tested were shown to improve lymphoproliferative response. Further, the combinations rcaIL-12/rcaIL-2 and rcaIL-12/rcaIL-15 promoted a decrease in programmed cell death protein 1 (PD-1) expression in lymphocytes. These same combinations of cytokines and rcaIL-12/rcasIL-10R1 induced IFN-γ and TNF-α production in PBMCs. Furthermore, the combination IL-12/IL-15 led to an increased in T-bet expression in lymphocytes. These findings are encouraging and indicate the use of rcaIL-12 and rcaIL-15 in future in vivo studies aimed at achieving polarization of cellular immune responses in dogs with leishmaniasis, which may contribute to the development of an effective treatment against CanL.engPublic Library of Sciencehttps://www.arca.fiocruz.br/handle/icict/39899Leishmaniose visceralInterleukin-12Interleukin-15LeucócitosAnimalsLeishmaniasis visceralInterleukin-12Interleukin-15LeukocytesAnimaisCombined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/40815/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCosta Sidnei Ferro Combined in 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dc.title.en.fl_str_mv Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis
title Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis
spellingShingle Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis
Costa, Sidnei Ferro
Leishmaniose visceral
Interleukin-12
Interleukin-15
Leucócitos
Animals
Leishmaniasis visceral
Interleukin-12
Interleukin-15
Leukocytes
Animais
title_short Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis
title_full Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis
title_fullStr Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis
title_full_unstemmed Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis
title_sort Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis
author Costa, Sidnei Ferro
author_facet Costa, Sidnei Ferro
Gomes, Vinícius Oliveira
Maciel, Marilene Oliveira dos Santos
Melo, Larissa Martins
Venturin, Gabriela Lovizutto
Bragato, Jaqueline Poleto
Rebech, Gabriela Torres
Santos, Catiule de Oliveira
Oliveira, Bárbara Maria Nascimento de
Oliveira, Geraldo Gileno de Sá
Lima, Valéria Marçal Felix de
author_role author
author2 Gomes, Vinícius Oliveira
Maciel, Marilene Oliveira dos Santos
Melo, Larissa Martins
Venturin, Gabriela Lovizutto
Bragato, Jaqueline Poleto
Rebech, Gabriela Torres
Santos, Catiule de Oliveira
Oliveira, Bárbara Maria Nascimento de
Oliveira, Geraldo Gileno de Sá
Lima, Valéria Marçal Felix de
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Sidnei Ferro
Gomes, Vinícius Oliveira
Maciel, Marilene Oliveira dos Santos
Melo, Larissa Martins
Venturin, Gabriela Lovizutto
Bragato, Jaqueline Poleto
Rebech, Gabriela Torres
Santos, Catiule de Oliveira
Oliveira, Bárbara Maria Nascimento de
Oliveira, Geraldo Gileno de Sá
Lima, Valéria Marçal Felix de
dc.subject.other.pt_BR.fl_str_mv Leishmaniose visceral
Interleukin-12
Interleukin-15
Leucócitos
Animals
topic Leishmaniose visceral
Interleukin-12
Interleukin-15
Leucócitos
Animals
Leishmaniasis visceral
Interleukin-12
Interleukin-15
Leukocytes
Animais
dc.subject.en.en.fl_str_mv Leishmaniasis visceral
Interleukin-12
Interleukin-15
Leukocytes
Animais
description São Paulo Research Foundation (FAPESP), process #2017/10906-8
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-04-17T12:58:38Z
dc.date.available.fl_str_mv 2020-04-17T12:58:38Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COSTA, Sidnei Ferro et al. Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis. PLoS Neglected Tropical Diseases, p. 1-21, 21 Jan. 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/40815
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.pt_BR.fl_str_mv 10.1371/journal.pntd.0008021
dc.identifier.eissn.pt_BR.fl_str_mv 1935-2735
identifier_str_mv COSTA, Sidnei Ferro et al. Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis. PLoS Neglected Tropical Diseases, p. 1-21, 21 Jan. 2020.
1935-2727
10.1371/journal.pntd.0008021
1935-2735
url https://www.arca.fiocruz.br/handle/icict/40815
dc.language.iso.fl_str_mv eng
language eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
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