IL-10 and IL-12 are the main regulatory cytokines in visceral leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Bacellar, Maria Olívia Amado Ramos
Data de Publicação: 2000
Outros Autores: D'Oliveira Junior, Argemiro, Jerônimo, Selma, Carvalho Filho, Edgar Marcelino de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/7814
Resumo: Texto completo: acesso restrito. p.1228–1231
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spelling Bacellar, Maria Olívia Amado RamosD'Oliveira Junior, ArgemiroJerônimo, SelmaCarvalho Filho, Edgar Marcelino deBacellar, Maria Olívia Amado RamosD'Oliveira Junior, ArgemiroJerônimo, SelmaCarvalho Filho, Edgar Marcelino de2013-01-11T14:50:46Z20001043-4666http://www.repositorio.ufba.br/ri/handle/ri/7814v. 12, n. 8Texto completo: acesso restrito. p.1228–1231Visceral leishmaniasis (VL) is characterized by the absence of cytokines such as IFN-γ and IL-12. Cure of VL is associated with a restoration of the ability to make these cytokines. The aim of the present study was to evaluate the role of IL-12 in the recovery of the ability to produce IFN-γ and to test whether or not IL-4 IL-10 and/or TGF-β could suppress IFN-γ production by PBMC from treated VL patients. High stimulation index (SI) of proliferation was observed in PBMC from subjects stimulated with Leishmania chagasi antigen (181±83). Neutralizing IL-12 inhibited lymphoproliferation [stimulation index (SI) of 210±114 to 1±0.6 (P<0.01)] and/or the production of IFN-γ [2792±402 pg/ml to 407±449 pg/ml (P<0.01)]. Recombinant IL-10 abrogated the lymphoproliferation (SI=2±3) while recombinant IL-4 or TGF-β had no effect on this response (147±22 and 194±12 respectively). IFN-γ was high when PBMCs were stimulated with L. chagasi (873±400 pg/ml) and this was abrogated by the addition of IL-10 (5±2 pg/ml). In contrast neither IL-4 or TGF-β suppressed IFN-γ production (837±244 pg/ml and 759±523 pg/ml). These results indicate that IL-12 plays an important role in the ability of treated VL patients to make IFN-γ and that IL-10 but not IL-4 or TGF-β inhibits this response.Submitted by Suelen Reis (suelen_suzane@hotmail.com) on 2013-01-11T14:50:46Z No. of bitstreams: 1 1-s2.0-S104346660090694X-main.pdf: 93695 bytes, checksum: 5a4a1f0c36faf35ea6d01fabccdf4571 (MD5)Made available in DSpace on 2013-01-11T14:50:46Z (GMT). No. of bitstreams: 1 1-s2.0-S104346660090694X-main.pdf: 93695 bytes, checksum: 5a4a1f0c36faf35ea6d01fabccdf4571 (MD5) Previous issue date: 2000http://dx.doi.org/10.1006/cyto.2000.0694reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAcytokinesinterleukin 10interleukin 12leishmaniasisvisceral leishmaniasisIL-10 and IL-12 are the main regulatory cytokines in visceral leishmaniasisCytokineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL1-s2.0-S104346660090694X-main.pdf1-s2.0-S104346660090694X-main.pdfapplication/pdf93695https://repositorio.ufba.br/bitstream/ri/7814/1/1-s2.0-S104346660090694X-main.pdf5a4a1f0c36faf35ea6d01fabccdf4571MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/7814/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52ri/78142022-08-08 13:17:34.934oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:17:34Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv IL-10 and IL-12 are the main regulatory cytokines in visceral leishmaniasis
dc.title.alternative.pt_BR.fl_str_mv Cytokine
title IL-10 and IL-12 are the main regulatory cytokines in visceral leishmaniasis
spellingShingle IL-10 and IL-12 are the main regulatory cytokines in visceral leishmaniasis
Bacellar, Maria Olívia Amado Ramos
cytokines
interleukin 10
interleukin 12
leishmaniasis
visceral leishmaniasis
title_short IL-10 and IL-12 are the main regulatory cytokines in visceral leishmaniasis
title_full IL-10 and IL-12 are the main regulatory cytokines in visceral leishmaniasis
title_fullStr IL-10 and IL-12 are the main regulatory cytokines in visceral leishmaniasis
title_full_unstemmed IL-10 and IL-12 are the main regulatory cytokines in visceral leishmaniasis
title_sort IL-10 and IL-12 are the main regulatory cytokines in visceral leishmaniasis
author Bacellar, Maria Olívia Amado Ramos
author_facet Bacellar, Maria Olívia Amado Ramos
D'Oliveira Junior, Argemiro
Jerônimo, Selma
Carvalho Filho, Edgar Marcelino de
author_role author
author2 D'Oliveira Junior, Argemiro
Jerônimo, Selma
Carvalho Filho, Edgar Marcelino de
author2_role author
author
author
dc.contributor.author.fl_str_mv Bacellar, Maria Olívia Amado Ramos
D'Oliveira Junior, Argemiro
Jerônimo, Selma
Carvalho Filho, Edgar Marcelino de
Bacellar, Maria Olívia Amado Ramos
D'Oliveira Junior, Argemiro
Jerônimo, Selma
Carvalho Filho, Edgar Marcelino de
dc.subject.por.fl_str_mv cytokines
interleukin 10
interleukin 12
leishmaniasis
visceral leishmaniasis
topic cytokines
interleukin 10
interleukin 12
leishmaniasis
visceral leishmaniasis
description Texto completo: acesso restrito. p.1228–1231
publishDate 2000
dc.date.issued.fl_str_mv 2000
dc.date.accessioned.fl_str_mv 2013-01-11T14:50:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7814
dc.identifier.issn.none.fl_str_mv 1043-4666
dc.identifier.number.pt_BR.fl_str_mv v. 12, n. 8
identifier_str_mv 1043-4666
v. 12, n. 8
url http://www.repositorio.ufba.br/ri/handle/ri/7814
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1006/cyto.2000.0694
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/7814/1/1-s2.0-S104346660090694X-main.pdf
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