Interaction of Bothrops jararaca venom metalloproteinases with protein inhibitors

Detalhes bibliográficos
Autor(a) principal: Asega, Amanda F.
Data de Publicação: 2014
Outros Autores: Oliveira, Ana K., Menezes, Milene C., Ferreira, Ana Gisele C. Neves, Serrano, Solange M. T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10692
Resumo: Instituto Butantan. Laboratório Especial de Toxinologia Aplicada-CeTICS. São Paulo, SP, Brasil.
id CRUZ_0ab22f2b3ed5ca516129eb4bef5cf3ec
oai_identifier_str oai:www.arca.fiocruz.br:icict/10692
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Asega, Amanda F.Oliveira, Ana K.Menezes, Milene C.Ferreira, Ana Gisele C. NevesSerrano, Solange M. T.2015-06-08T14:01:51Z2015-06-08T14:01:51Z2014ASEGA, Amanda F. et al. Interaction of Bothrops jararaca venom metalloproteinases with protein inhibitors. Toxicon, v. 80, p.1-8, Mar. 2014.0041-0101https://www.arca.fiocruz.br/handle/icict/1069210.1016/j.toxicon.2014.01.001engElsevierInibiçãoHemorrhageInhibitionMetalloproteinaseProteolysisSnake venomHemorragiaProteolysisMetalloproteinaseVenenos de SerpentesInteraction of Bothrops jararaca venom metalloproteinases with protein inhibitorsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Butantan. Laboratório Especial de Toxinologia Aplicada-CeTICS. São Paulo, SP, Brasil.Instituto Butantan. Laboratório Especial de Toxinologia Aplicada-CeTICS. São Paulo, SP, Brasil.Instituto Butantan. Laboratório Especial de Toxinologia Aplicada-CeTICS. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxonologia. Rio de Janeiro, RJ, Brasil.Instituto Butantan. Laboratório Especial de Toxinologia Aplicada-CeTICS. São Paulo, SP, Brasil.Snake venom metalloproteinases (SVMPs) play important roles in the local and systemic hemorrhage observed upon envenomation. In a previous study on the structural elements important for the activities of HF3 (highly hemorrhagic, P-III-SVMP), bothropasin (hemorrhagic, P-III-SVMP) and BJ-PI (non-hemorrhagic, P-I-SVMP), from Bothrops jararaca, it was demonstrated that they differ in their proteolysis profile of plasma and extracellular matrix proteins. In this study, we evaluated the ability of proteins DM43 and a2- macroglobulin to interfere with the proteolytic activity of these SVMPs on fibrinogen and collagen VI and with their ability to induce hemorrhage. DM43 inhibited the proteolytic activity of bothropasin and BJ-PI but not that of HF3, and was not cleaved the three proteinases. On the other hand, a2-macroglobulin did not inhibit any of the proteinases and was rather cleaved by them. In agreement with these findings, binding analysis showed interaction of bothropasin and BJ-PI but not HF3 to DM43 while none of the proteinases bound to a2-macroglobulin. Moreover, DM43 promoted partial inhibition of the hemorrhagic activity of bothropasin but not that of HF3. Our results demonstrate that metalloproteinases of B. jararaca venom showing different domain composition, glycosylation level and hemorrhagic potency show variable susceptibilities to protein inhibitors.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10692/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALana_ferreiraetal_IOC_2014.pdfapplication/pdf1325949https://www.arca.fiocruz.br/bitstream/icict/10692/2/ana_ferreiraetal_IOC_2014.pdf8a1af05b523f11b0f45b9059129701abMD52TEXTana_ferreiraetal_IOC_2014.pdf.txtana_ferreiraetal_IOC_2014.pdf.txtExtracted texttext/plain38150https://www.arca.fiocruz.br/bitstream/icict/10692/3/ana_ferreiraetal_IOC_2014.pdf.txt56d121f81407bf9513176606f8745d55MD53icict/106922022-06-24 12:17:36.144oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:17:36Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Interaction of Bothrops jararaca venom metalloproteinases with protein inhibitors
title Interaction of Bothrops jararaca venom metalloproteinases with protein inhibitors
spellingShingle Interaction of Bothrops jararaca venom metalloproteinases with protein inhibitors
Asega, Amanda F.
Inibição
Hemorrhage
Inhibition
Metalloproteinase
Proteolysis
Snake venom
Hemorragia
Proteolysis
Metalloproteinase
Venenos de Serpentes
title_short Interaction of Bothrops jararaca venom metalloproteinases with protein inhibitors
title_full Interaction of Bothrops jararaca venom metalloproteinases with protein inhibitors
title_fullStr Interaction of Bothrops jararaca venom metalloproteinases with protein inhibitors
title_full_unstemmed Interaction of Bothrops jararaca venom metalloproteinases with protein inhibitors
title_sort Interaction of Bothrops jararaca venom metalloproteinases with protein inhibitors
author Asega, Amanda F.
author_facet Asega, Amanda F.
Oliveira, Ana K.
Menezes, Milene C.
Ferreira, Ana Gisele C. Neves
Serrano, Solange M. T.
author_role author
author2 Oliveira, Ana K.
Menezes, Milene C.
Ferreira, Ana Gisele C. Neves
Serrano, Solange M. T.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Asega, Amanda F.
Oliveira, Ana K.
Menezes, Milene C.
Ferreira, Ana Gisele C. Neves
Serrano, Solange M. T.
dc.subject.other.pt_BR.fl_str_mv Inibição
topic Inibição
Hemorrhage
Inhibition
Metalloproteinase
Proteolysis
Snake venom
Hemorragia
Proteolysis
Metalloproteinase
Venenos de Serpentes
dc.subject.en.pt_BR.fl_str_mv Hemorrhage
Inhibition
Metalloproteinase
Proteolysis
Snake venom
dc.subject.decs.pt_BR.fl_str_mv Hemorragia
Proteolysis
Metalloproteinase
Venenos de Serpentes
description Instituto Butantan. Laboratório Especial de Toxinologia Aplicada-CeTICS. São Paulo, SP, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-08T14:01:51Z
dc.date.available.fl_str_mv 2015-06-08T14:01:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ASEGA, Amanda F. et al. Interaction of Bothrops jararaca venom metalloproteinases with protein inhibitors. Toxicon, v. 80, p.1-8, Mar. 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10692
dc.identifier.issn.pt_BR.fl_str_mv 0041-0101
dc.identifier.doi.pt_BR.fl_str_mv 10.1016/j.toxicon.2014.01.001
identifier_str_mv ASEGA, Amanda F. et al. Interaction of Bothrops jararaca venom metalloproteinases with protein inhibitors. Toxicon, v. 80, p.1-8, Mar. 2014.
0041-0101
10.1016/j.toxicon.2014.01.001
url https://www.arca.fiocruz.br/handle/icict/10692
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/10692/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/10692/2/ana_ferreiraetal_IOC_2014.pdf
https://www.arca.fiocruz.br/bitstream/icict/10692/3/ana_ferreiraetal_IOC_2014.pdf.txt
bitstream.checksum.fl_str_mv 7d48279ffeed55da8dfe2f8e81f3b81f
8a1af05b523f11b0f45b9059129701ab
56d121f81407bf9513176606f8745d55
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813008889943162880

Registros relacionados