Use of immunoblotting as an alternative method for serogrouping Leptospira.

Detalhes bibliográficos
Autor(a) principal: Doungchawee, Galayanee
Data de Publicação: 2007
Outros Autores: Sirawaraporn, Worachart, Ko, Albert Icksang, Kongtim, Suraphol, Naigowit, Pimjai, Visith Thongboonkerd
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12142
Resumo: Mahidol University. Faculty of Science. Department of Pathobiology. Bangkok, Thailand
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spelling Doungchawee, GalayaneeSirawaraporn, WorachartKo, Albert IcksangKongtim, SurapholNaigowit, PimjaiVisith Thongboonkerd2015-11-10T12:55:07Z2015-11-10T12:55:07Z2007DOUNGCHAWEE, D. et al. Use of immunoblotting as an alternative method for serogrouping Leptospira. Journal of Medical Microbiology, v. 56, n. 5, p. 587-592, 2007.0022-2615https://www.arca.fiocruz.br/handle/icict/1214210.1099/jmm.0.47143-0engMicrobiology SocietyUse of immunoblotting as an alternative method for serogrouping Leptospira.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMahidol University. Faculty of Science. Department of Pathobiology. Bangkok, ThailandMahidol University. Faculty of Science. Department of Biochemistry. Bangkok, ThailandFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Weill Medical College of Cornell University. Division of International Medicine and Infectious Disease. New York, NY, USAMahidol University. Faculty of Science. Department of Pathobiology. Bangkok, ThailandNational Institute of Health. Research Center for Leptospira Laboratory. Nonthaburi, ThailandMahidol University. Medical Molecular Biology Unit. Office for Research and Development. Department of Medicine Siriraj Hospital. Bangkok, ThailandLeptospirosis is a worldwide zoonotic disease caused by a spirochaete bacterium, Leptospira. Serological detection of this micro-organism basically relies on a conventional microscopic agglutination test (MAT), which has some limitations and disadvantages. In the present study, immunoblotting has been applied as an alternative method for differentiating serogroups and serovars of leptospires. Leptospiral whole-cell lysates from a total of 26 serovars were subjected to immunoblotting using rabbit antisera against individual serovars. The findings clearly demonstrated that the pattern of immunoreactive bands could be used to differentiate between leptospires of different serogroups, consistent with MAT results. There was a multi-band pattern that was unique for the pathogenic Leptospira antigens and was not observed in the non-pathogenic Leptospira biflexa and non-leptospiral bacteria (i.e. Escherichia coli, Burkholderia pseudomallei and Helicobacter pylori). For pathogenic Leptospira species, a prominent smear-like band at approximately 19-30 kDa was present when the antigens were probed with the homologous antisera. The molecular size of the prominent band, although it showed a cross-reaction between members within the same serogroup, differed among different serovars. The results obtained from polyclonal antibodies (antisera) were confirmed using mAb. With its simplicity and safety of experimental procedures, it is proposed that immunoblotting may potentially be useful as an alternative method for differentiating between serogroups of leptospires.ImmunoblottingLeptospira/classificaçãoLeptospira/imunologiaSorotipagem/métodosAnticorpos Antibacterianos/imunologiaAntígenos de Bactérias/análiseAntígenos de Bactérias/imunologiaBurkholderia pseudomallei/imunologiaEpitoposEscherichia coli/imunologiaHelicobacter pylori/imunologiaPeso Molecularinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/12142/1/license.txt8b4c200b4e10021c5683c6ccaba07169MD51ORIGINALDoungchawee G Use of immunoblotting as an....pdfDoungchawee G Use of immunoblotting as an....pdfapplication/pdf155445https://www.arca.fiocruz.br/bitstream/icict/12142/2/Doungchawee%20%20G%20Use%20of%20immunoblotting%20as%20an....pdfc9aa29f37f22843335663c13e0f3cb52MD52TEXTDoungchawee G Use of immunoblotting as an....pdf.txtDoungchawee G Use of immunoblotting as an....pdf.txtExtracted texttext/plain24948https://www.arca.fiocruz.br/bitstream/icict/12142/3/Doungchawee%20%20G%20Use%20of%20immunoblotting%20as%20an....pdf.txt695c1478dcae3d753d5fcc3eefa8a97aMD53icict/121422023-03-15 14:33:22.811oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:22Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Use of immunoblotting as an alternative method for serogrouping Leptospira.
title Use of immunoblotting as an alternative method for serogrouping Leptospira.
spellingShingle Use of immunoblotting as an alternative method for serogrouping Leptospira.
Doungchawee, Galayanee
Immunoblotting
Leptospira/classificação
Leptospira/imunologia
Sorotipagem/métodos
Anticorpos Antibacterianos/imunologia
Antígenos de Bactérias/análise
Antígenos de Bactérias/imunologia
Burkholderia pseudomallei/imunologia
Epitopos
Escherichia coli/imunologia
Helicobacter pylori/imunologia
Peso Molecular
title_short Use of immunoblotting as an alternative method for serogrouping Leptospira.
title_full Use of immunoblotting as an alternative method for serogrouping Leptospira.
title_fullStr Use of immunoblotting as an alternative method for serogrouping Leptospira.
title_full_unstemmed Use of immunoblotting as an alternative method for serogrouping Leptospira.
title_sort Use of immunoblotting as an alternative method for serogrouping Leptospira.
author Doungchawee, Galayanee
author_facet Doungchawee, Galayanee
Sirawaraporn, Worachart
Ko, Albert Icksang
Kongtim, Suraphol
Naigowit, Pimjai
Visith Thongboonkerd
author_role author
author2 Sirawaraporn, Worachart
Ko, Albert Icksang
Kongtim, Suraphol
Naigowit, Pimjai
Visith Thongboonkerd
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Doungchawee, Galayanee
Sirawaraporn, Worachart
Ko, Albert Icksang
Kongtim, Suraphol
Naigowit, Pimjai
Visith Thongboonkerd
dc.subject.decs.pt_BR.fl_str_mv Immunoblotting
Leptospira/classificação
Leptospira/imunologia
Sorotipagem/métodos
Anticorpos Antibacterianos/imunologia
Antígenos de Bactérias/análise
Antígenos de Bactérias/imunologia
Burkholderia pseudomallei/imunologia
Epitopos
Escherichia coli/imunologia
Helicobacter pylori/imunologia
Peso Molecular
topic Immunoblotting
Leptospira/classificação
Leptospira/imunologia
Sorotipagem/métodos
Anticorpos Antibacterianos/imunologia
Antígenos de Bactérias/análise
Antígenos de Bactérias/imunologia
Burkholderia pseudomallei/imunologia
Epitopos
Escherichia coli/imunologia
Helicobacter pylori/imunologia
Peso Molecular
description Mahidol University. Faculty of Science. Department of Pathobiology. Bangkok, Thailand
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2015-11-10T12:55:07Z
dc.date.available.fl_str_mv 2015-11-10T12:55:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DOUNGCHAWEE, D. et al. Use of immunoblotting as an alternative method for serogrouping Leptospira. Journal of Medical Microbiology, v. 56, n. 5, p. 587-592, 2007.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12142
dc.identifier.issn.none.fl_str_mv 0022-2615
dc.identifier.doi.none.fl_str_mv 10.1099/jmm.0.47143-0
identifier_str_mv DOUNGCHAWEE, D. et al. Use of immunoblotting as an alternative method for serogrouping Leptospira. Journal of Medical Microbiology, v. 56, n. 5, p. 587-592, 2007.
0022-2615
10.1099/jmm.0.47143-0
url https://www.arca.fiocruz.br/handle/icict/12142
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Microbiology Society
publisher.none.fl_str_mv Microbiology Society
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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