Leptospiral proteins recognized during the humoral immune response to leptospirosis in humans.

Detalhes bibliográficos
Autor(a) principal: Guerreiro, Hygia
Data de Publicação: 2001
Outros Autores: Croda, Julio Henrique Rosa, Flannery, Brendan, Mazel, Mary, Matsunaga, James, Reis, Mitermayer Galvão dos, Levett, Paul N., Ko, Albert Icksang, Haake, David A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/5532
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
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spelling Guerreiro, HygiaCroda, Julio Henrique RosaFlannery, BrendanMazel, MaryMatsunaga, JamesReis, Mitermayer Galvão dosLevett, Paul N.Ko, Albert IcksangHaake, David A.2012-09-19T19:47:16Z2012-09-19T19:47:16Z2001GUERREIRO, H. et al. Leptospiral proteins recognized during the humoral immune response to leptospirosis in humans. Infection and Immunity, v. 69, n. 8, p. 4958-68, Aug. 2001.0019-9567https://www.arca.fiocruz.br/handle/icict/553210.1128/IAI.69.8.4958–4968.2001engLeptospiral proteins recognized during the humoral immune response to leptospirosis in humans.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversity of California at Berkeley. School of Public Health. Berkeley, CA, United States of AmericaVeterans Affairs Greater Los Angeles Healthcare System.Division of Infectious Diseases. Los Angeles, CA, United States of AmericaLos Angeles Healthcare System. Los Angeles, CA, United States of AmericaFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversity of the West Indies. School of Clinical Medicine and Research. Bridgetown, BarbadosFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilVeterans Affairs Greater Los Angeles Healthcare System. Division of Infectious Diseases. Los Angeles, CA, United States of AmericaLeptospirosis is an emerging zoonosis caused by pathogenic spirochetes belonging to the genus Leptospira. An understanding of leptospiral protein expression regulation is needed to develop new immunoprotective and serodiagnostic strategies. We used the humoral immune response during human leptospirosis as a reporter of protein antigens expressed during infection. Qualitative and quantitative immunoblot analysis was performed using sera from 105 patients from Brazil and Barbados. Sera from patients with other diseases and healthy individuals were evaluated as controls. Seven proteins, p76, p62, p48, p45, p41, p37, and p32, were identified as targets of the humoral response during natural infection. In both acute and convalescent phases of illness, antibodies to lipopolysaccharide were predominantly immunoglobulin M (IgM) while antibodies to proteins were exclusively IgG. Anti-p32 reactivity had the greatest sensitivity and specificity: positive reactions were observed in 37 and 84% of acute- and convalescent-phase sera, respectively, while only 5% of community control individuals demonstrated positive reactions. Six immunodominant antigens were expressed by all pathogenic leptospiral strains tested; only p37 was inconsistently expressed. Two-dimensional immunoblots identified four of the seven infection-associated antigens as being previously characterized proteins: LipL32 (the major outer membrane lipoprotein), LipL41 (a surface-exposed outer membrane lipoprotein), and heat shock proteins GroEL and DnaK. Fractionation studies demonstrated LipL32 and LipL41 reactivity in the outer membrane fraction and GroEL and DnaK in the cytoplasmic fraction, while p37 appeared to be a soluble periplasmic protein. Most of the other immunodominant proteins, including p48 and p45, were localized to the inner membrane. These findings indicate that leptospiral proteins recognized during natural infection are potentially useful for serodiagnosis and may serve as targets for vaccine design.Anticorpos Antibacterianos/sangueProteínas de Bactérias/imunologiaLeptospirose/imunologiaAnimaisAnticorpos Antibacterianos/imunologiaAntígenos de BactériasFracionamento CelularEletroforese em Gel Bidimensional/métodosHumanosLeptospira/imunologiaLeptospirose/sangueCoelhosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALGuerreiro H Leptospiral proteins recognized ....pdfGuerreiro H Leptospiral proteins recognized ....pdfapplication/pdf1757480https://www.arca.fiocruz.br/bitstream/icict/5532/1/Guerreiro%20H%20Leptospiral%20proteins%20recognized%20....pdf8c766e77fd285d31b0bf9aee9d12605bMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82008https://www.arca.fiocruz.br/bitstream/icict/5532/2/license.txt06db3f0df647fd9ddff657403bbe0ee2MD52TEXTGuerreiro H Leptospiral proteins recognized ....pdf.txtGuerreiro H Leptospiral proteins recognized ....pdf.txtExtracted texttext/plain66368https://www.arca.fiocruz.br/bitstream/icict/5532/5/Guerreiro%20H%20Leptospiral%20proteins%20recognized%20....pdf.txtb57f0b78cfb95495e671382b42a5cc9aMD55THUMBNAILGuerreiro H Leptospiral proteins recognized ....pdf.jpgGuerreiro H Leptospiral proteins recognized ....pdf.jpgGenerated Thumbnailimage/jpeg2340https://www.arca.fiocruz.br/bitstream/icict/5532/4/Guerreiro%20H%20Leptospiral%20proteins%20recognized%20....pdf.jpgd3db54df7c6dec585ef2e36ac1840722MD54icict/55322023-03-15 14:33:01.312oai:www.arca.fiocruz.br:icict/5532TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGFzc2luYXIgZSBlbnRyZWdhciBlc3RhIGxpY2Vuw6dhLCBvL2EgU3IuL1NyYS4gKGF1dG9yIG91IGRldGVudG9yIGRvcyBkaXJlaXRvcyBkbyBhdXRvcik6CgpBbyBjb25jb3JkYXIgZSBhY2VpdGFyIGVzdGEgbGljZW7Dp2Egdm9jw6ogKGF1dG9yIG91IGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyk6CgphKSBEZWNsYXJhIHF1ZSBjb25oZWNlIGEgcG9sw610aWNhIGRlIGNvcHlyaWdodCBkYSBlZGl0b3JhIGRvIHNldSBkb2N1bWVudG8uCgpiKSBEZWNsYXJhIHF1ZSBjb25oZWNlIGUgYWNlaXRhIGFzIERpcmV0cml6ZXMgcGFyYSBvIFJlcG9zaXTDs3JpbyBJbnN0aXR1Y2lvbmFsIGRhIEZ1bmRhw6fDo28gT3N3YWxkbyBDcnV6IChGSU9DUlVaKS4KCmMpIENvbmNlZGUgw6AgRklPQ1JVWiBvIGRpcmVpdG8gbsOjby1leGNsdXNpdm8gZGUgYXJxdWl2YXIsIHJlcHJvZHV6aXIsIGNvbnZlcnRlciAoY29tbyBkZWZpbmlkbyBhIHNlZ3VpciksIGNvbXVuaWNhcgogCmUvb3UgZGlzdHJpYnVpciBubyBSZXBvc2l0w7NyaW8gZGEgRklPQ1JVWiwgbyBkb2N1bWVudG8gZW50cmVndWUgKGluY2x1aW5kbyBvIHJlc3Vtby9hYnN0cmFjdCkgZW0gZm9ybWF0byBkaWdpdGFsIG91IAoKcG9yIHF1YWxxdWVyIG91dHJvIG1laW8uCgpkKSBEZWNsYXJhIHF1ZSBhdXRvcml6YSBhIEZJT0NSVVogYSBhcnF1aXZhciBtYWlzIGRlIHVtYSBjw7NwaWEgZGVzdGUgZG9jdW1lbnRvIGUgY29udmVydMOqLWxvLCBzZW0gYWx0ZXJhciBvIHNldSBjb250ZcO6ZG8sIAoKcGFyYSBxdWFscXVlciBmb3JtYXRvIGRlIGFycXVpdm8sIG1laW8gb3Ugc3Vwb3J0ZSwgcGFyYSBlZmVpdG9zIGRlIHNlZ3VyYW7Dp2EsIHByZXNlcnZhw6fDo28gKGJhY2t1cCkgZSBhY2Vzc28uCgplKSBEZWNsYXJhIHF1ZSBvIGRvY3VtZW50byBzdWJtZXRpZG8gw6kgbyBzZXUgdHJhYmFsaG8gb3JpZ2luYWwsIGUgcXVlIGRldMOpbSBvIGRpcmVpdG8gZGUgY29uY2VkZXIgYSB0ZXJjZWlyb3Mgb3MgZGlyZWl0b3MgCgpjb250aWRvcyBuZXN0YSBsaWNlbsOnYS4gRGVjbGFyYSB0YW1iw6ltIHF1ZSBhIGVudHJlZ2EgZG8gZG9jdW1lbnRvIG7Do28gaW5mcmluZ2Ugb3MgZGlyZWl0b3MgZGUgcXVhbHF1ZXIgb3V0cmEgcGVzc29hIG91IGVudGlkYWRlLgoKZikgRGVjbGFyYSBxdWUsIG5vIGNhc28gZG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyBjb250ZXIgbWF0ZXJpYWwgZG8gcXVhbCBuw6NvIGRldMOpbSBvcyBkaXJlaXRvcyBkZSBhdXRvciwgb2J0ZXZlIGEgYXV0b3JpemHDp8OjbyAKCmlycmVzdHJpdGEgZG8gcmVzcGVjdGl2byBkZXRlbnRvciBkZXNzZXMgZGlyZWl0b3MsIHBhcmEgY2VkZXIgYSBGSU9DUlVaIG9zIGRpcmVpdG9zIHJlcXVlcmlkb3MgcG9yIGVzdGEgTGljZW7Dp2EgZSBhdXRvcml6YXIgYSAKCnV0aWxpesOhLWxvcyBsZWdhbG1lbnRlLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGVzc2UgbWF0ZXJpYWwgY3Vqb3MgZGlyZWl0b3Mgc8OjbyBkZSB0ZXJjZWlyb3MgZXN0w6EgY2xhcmFtZW50ZSBpZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyAKCm5vIHRleHRvIG91IGNvbnRlw7pkbyBkbyBkb2N1bWVudG8gZW50cmVndWUuCgpnKSBTRSBPIERPQ1VNRU5UTyBFTlRSRUdVRSDDiSBCQVNFQURPIEVNIFRSQUJBTEhPIEZJTkFOQ0lBRE8gT1UgQVBPSUFETyBQT1IgT1VUUkEgSU5TVElUVUnDh8ODTyBRVUUgTsODTyBBIEZJT0NSVVosIERFQ0xBUkEgUVVFIENVTVBSSVUgCgpRVUFJU1FVRVIgT0JSSUdBw4fDlUVTIEVYSUdJREFTIFBFTE8gUkVTUEVDVElWTyBDT05UUkFUTyBPVSBBQ09SRE8uIEEgRklPQ1JVWiBpZGVudGlmaWNhcsOhIGNsYXJhbWVudGUgbyhzKSBub21lKHMpIGRvKHMpIGF1dG9yKGVzKSBkb3MgCgpkaXJlaXRvcyBkbyBkb2N1bWVudG8gZW50cmVndWUgZSBuw6NvIGZhcsOhIHF1YWxxdWVyIGFsdGVyYcOnw6NvLCBwYXJhIGFsw6ltIGRvIHByZXZpc3RvIG5hIGFsw61uZWEgYykuCg==Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:01Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Leptospiral proteins recognized during the humoral immune response to leptospirosis in humans.
title Leptospiral proteins recognized during the humoral immune response to leptospirosis in humans.
spellingShingle Leptospiral proteins recognized during the humoral immune response to leptospirosis in humans.
Guerreiro, Hygia
Anticorpos Antibacterianos/sangue
Proteínas de Bactérias/imunologia
Leptospirose/imunologia
Animais
Anticorpos Antibacterianos/imunologia
Antígenos de Bactérias
Fracionamento Celular
Eletroforese em Gel Bidimensional/métodos
Humanos
Leptospira/imunologia
Leptospirose/sangue
Coelhos
title_short Leptospiral proteins recognized during the humoral immune response to leptospirosis in humans.
title_full Leptospiral proteins recognized during the humoral immune response to leptospirosis in humans.
title_fullStr Leptospiral proteins recognized during the humoral immune response to leptospirosis in humans.
title_full_unstemmed Leptospiral proteins recognized during the humoral immune response to leptospirosis in humans.
title_sort Leptospiral proteins recognized during the humoral immune response to leptospirosis in humans.
author Guerreiro, Hygia
author_facet Guerreiro, Hygia
Croda, Julio Henrique Rosa
Flannery, Brendan
Mazel, Mary
Matsunaga, James
Reis, Mitermayer Galvão dos
Levett, Paul N.
Ko, Albert Icksang
Haake, David A.
author_role author
author2 Croda, Julio Henrique Rosa
Flannery, Brendan
Mazel, Mary
Matsunaga, James
Reis, Mitermayer Galvão dos
Levett, Paul N.
Ko, Albert Icksang
Haake, David A.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Guerreiro, Hygia
Croda, Julio Henrique Rosa
Flannery, Brendan
Mazel, Mary
Matsunaga, James
Reis, Mitermayer Galvão dos
Levett, Paul N.
Ko, Albert Icksang
Haake, David A.
dc.subject.decs.pt_BR.fl_str_mv Anticorpos Antibacterianos/sangue
Proteínas de Bactérias/imunologia
Leptospirose/imunologia
Animais
Anticorpos Antibacterianos/imunologia
Antígenos de Bactérias
Fracionamento Celular
Eletroforese em Gel Bidimensional/métodos
Humanos
Leptospira/imunologia
Leptospirose/sangue
Coelhos
topic Anticorpos Antibacterianos/sangue
Proteínas de Bactérias/imunologia
Leptospirose/imunologia
Animais
Anticorpos Antibacterianos/imunologia
Antígenos de Bactérias
Fracionamento Celular
Eletroforese em Gel Bidimensional/métodos
Humanos
Leptospira/imunologia
Leptospirose/sangue
Coelhos
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2012-09-19T19:47:16Z
dc.date.available.fl_str_mv 2012-09-19T19:47:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GUERREIRO, H. et al. Leptospiral proteins recognized during the humoral immune response to leptospirosis in humans. Infection and Immunity, v. 69, n. 8, p. 4958-68, Aug. 2001.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/5532
dc.identifier.issn.none.fl_str_mv 0019-9567
dc.identifier.doi.none.fl_str_mv 10.1128/IAI.69.8.4958–4968.2001
identifier_str_mv GUERREIRO, H. et al. Leptospiral proteins recognized during the humoral immune response to leptospirosis in humans. Infection and Immunity, v. 69, n. 8, p. 4958-68, Aug. 2001.
0019-9567
10.1128/IAI.69.8.4958–4968.2001
url https://www.arca.fiocruz.br/handle/icict/5532
dc.language.iso.fl_str_mv eng
language eng
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