Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity Schistosoma mansoni infections in endemic area in Brazil

Silva-Moraes, Vanessa; Shollenberger, Lisa Marie; Castro-Borges, William; Rabello, Ana Lucia Teles; Harn, Donald A.; Medeiros, Lia Carolina Soares; Jeremias, Wander de Jesus; Siqueira, Liliane Maria Vidal; Pereira, Caroline Stephane Salviano; Pedrosa, Maria Luysa Camargos; Almeida, Nathalie Bonatti Franco; Almeida, Aureo; Lambertucci, Jose Roberto; Carneiro, Nídia Francisca de Figueiredo; Coelho, Paulo Marcos Zech; Grenfell, Rafaella Fortini Queiroz

Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity Schistosoma mansoni infections in endemic area in Brazil

Detalhes bibliográficos
Autor(a) principal:	Silva-Moraes, Vanessa
Data de Publicação:	2019
Outros Autores:	Shollenberger, Lisa Marie, Castro-Borges, William, Rabello, Ana Lucia Teles, Harn, Donald A., Medeiros, Lia Carolina Soares, Jeremias, Wander de Jesus, Siqueira, Liliane Maria Vidal, Pereira, Caroline Stephane Salviano, Pedrosa, Maria Luysa Camargos, Almeida, Nathalie Bonatti Franco, Almeida, Aureo, Lambertucci, Jose Roberto, Carneiro, Nídia Francisca de Figueiredo, Coelho, Paulo Marcos Zech, Grenfell, Rafaella Fortini Queiroz
Tipo de documento:	Artigo
Idioma:	por
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/34288
Resumo:	Fundação Oswaldo Cruz. Intituto René Rachou. Biologia do Schistosoma Mansoni e sua interação com o hospedeiro. Belo Horizonte, MG, Brasil.

Metadados do item

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network_acronym_str	CRUZ
network_name_str	Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str	2135
spelling	Silva-Moraes, VanessaShollenberger, Lisa MarieCastro-Borges, WilliamRabello, Ana Lucia TelesHarn, Donald A.Medeiros, Lia Carolina SoaresJeremias, Wander de JesusSiqueira, Liliane Maria VidalPereira, Caroline Stephane SalvianoPedrosa, Maria Luysa CamargosAlmeida, Nathalie Bonatti FrancoAlmeida, AureoLambertucci, Jose RobertoCarneiro, Nídia Francisca de FigueiredoCoelho, Paulo Marcos ZechGrenfell, Rafaella Fortini Queiroz2019-07-19T14:21:06Z2019-07-19T14:21:06Z2019SILVA-MORAES, Vanessa et al. Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity Schistosoma mansoni infections in endemic area in Brazil. PLoS Neglected Tropical Diseases, v. 13, n. 3, p. 1-27, 2019.1935-2735https://www.arca.fiocruz.br/handle/icict/3428810.1371/journal.pntd.00069741935-2735porPublic Library of ScienceSchistosoma mansoniEnzyme-Linked Immunosorbent AssaySchistosomiasis mansoniImmunologic TestsAntigensReagent Kits, DiagnosticEnsayo de Inmunoadsorción EnzimáticaEsquistosomiasis mansoniPruebas InmunológicasJuego de Reactivos para DiagnósticoEnsaio de Imunoadsorção EnzimáticaEsquistossomose mansoniTestes ImunológicosAntígenosKit de Reagentes para DiagnósticoSerological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity Schistosoma mansoni infections in endemic area in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Intituto René Rachou. Biologia do Schistosoma Mansoni e sua interação com o hospedeiro. Belo Horizonte, MG, Brasil.Department of Infectious Diseases. College of Veterinary Medicine. University of Georgia. Athens, Georgia, United States of America.Universidade Federal de Ouro Preto. Laboratório de Enzimologia e Proteomica. Ouro Preto, Minas Gerais, Brasil.Fundação Oswaldo Cruz. Intituto René Rachou. Grupo de Pesquisas Clínicas e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.Department of Infectious Diseases. College of Veterinary Medicine. University of Georgia. Athens, Georgia, United States of America.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Intituto René Rachou. Biologia do Schistosoma Mansoni e sua interação com o hospedeiro. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Intituto René Rachou. Biologia do Schistosoma Mansoni e sua interação com o hospedeiro. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Intituto René Rachou. Biologia do Schistosoma Mansoni e sua interação com o hospedeiro. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Intituto René Rachou. Biologia do Schistosoma Mansoni e sua interação com o hospedeiro. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Intituto René Rachou. Biologia do Schistosoma Mansoni e sua interação com o hospedeiro. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Intituto René Rachou. Biologia do Schistosoma Mansoni e sua interação com o hospedeiro. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brasil.Universidade Federal de Montes Claros. Faculdade de Medicina. Montes Claros, MG, Brasil.Fundação Oswaldo Cruz. Intituto René Rachou. Biologia do Schistosoma Mansoni e sua interação com o hospedeiro. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Intituto René Rachou. Biologia do Schistosoma Mansoni e sua interação com o hospedeiro. Belo Horizonte, MG, Brasil. / Department of Infectious Diseases. College of Veterinary Medicine. University of Georgia. Athens, Georgia, United States of America.Despite decades of use of control programs, schistosomiasis remains a global public health problem. To further reduce prevalence and intensity of infection, or to achieve the goal of elimination in low-endemic areas, there needs to be better diagnostic tools to detect lowintensity infections in low-endemic areas in Brazil. The rationale for development of new diagnostic tools is that the current standard test Kato-Katz (KK) is not sensitive enough to detect low-intensity infections in low-endemic areas. In order to develop new diagnostic tools, we employed a proteomics approach to identify biomarkers associated with schistosome- specific immune responses in hopes of developing sensitive and specific new methods for immunodiagnosis. Immunoproteomic analyses were performed on egg extracts of Schistosoma mansoni using pooled sera from infected or non-infected individuals from a low-endemic area of Brazil. Cross reactivity with other soil-transmitted helminths (STH) was determined using pooled sera from individuals uniquely infected with different helminths. Using this approach, we identified 23 targets recognized by schistosome acute and chronic sera samples. To identify immunoreactive targets that were likely glycan epitopes, we compared these targets to the immunoreactivity of spots treated with sodium metaperiodate oxidation of egg extract. This treatment yielded 12/23 spots maintaining immunoreactivity, suggesting that they were protein epitopes. From these 12 spots, 11 spots cross-reacted with sera from individuals infected with other STH and 10 spots cross-reacted with the negative control group. Spot number 5 was exclusively immunoreactive with sera from S. mansoni-infected groups in native and deglycosylated conditions and corresponds to Major Egg Antigen (MEA). We expressed MEA as a recombinant protein and showed a similar recognition pattern to that of the native protein via western blot. IgG-ELISA gave a sensitivity of 87.10% and specificity of 89.09% represented by area under the ROC curve of 0.95. IgG-ELISA performed better than the conventional KK (2 slides), identifying 56/64 cases harboring 1–10 eggs per gram of feces that were undiagnosed by KK parasitological technique. The serological proteome approach was able to identify a new diagnostic candidate. The recombinant egg antigen provided good performance in IgG-ELISA to detect individuals with extreme low-intensity infections (1 egg per gram of feces). Therefore, the IgG-ELISA using this newly identified recombinant MEA can be a useful tool combined with other techniques in low-endemic areas to determine the true prevalence of schistosome infection that is underestimated by the KK method. Further, to overcome the complexity of ELISA in the field, a second generation of antibody-based rapid diagnostic tests (RDT) can be developed.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/34288/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjournal.pntd.000697.pdfjournal.pntd.000697.pdfapplication/pdf1902504https://www.arca.fiocruz.br/bitstream/icict/34288/2/journal.pntd.000697.pdf4ed08913175cd518c8a0e4a92a9a9da0MD52TEXTjournal.pntd.000697.pdf.txtjournal.pntd.000697.pdf.txtExtracted texttext/plain104249https://www.arca.fiocruz.br/bitstream/icict/34288/3/journal.pntd.000697.pdf.txt7d1a2ce3b66c8526de42ee700a783e70MD53icict/342882019-07-22 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dc.title.pt_BR.fl_str_mv	Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity Schistosoma mansoni infections in endemic area in Brazil
title	Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity Schistosoma mansoni infections in endemic area in Brazil
spellingShingle	Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity Schistosoma mansoni infections in endemic area in Brazil Silva-Moraes, Vanessa Schistosoma mansoni Enzyme-Linked Immunosorbent Assay Schistosomiasis mansoni Immunologic Tests Antigens Reagent Kits, Diagnostic Ensayo de Inmunoadsorción Enzimática Esquistosomiasis mansoni Pruebas Inmunológicas Juego de Reactivos para Diagnóstico Ensaio de Imunoadsorção Enzimática Esquistossomose mansoni Testes Imunológicos Antígenos Kit de Reagentes para Diagnóstico
title_short	Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity Schistosoma mansoni infections in endemic area in Brazil
title_full	Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity Schistosoma mansoni infections in endemic area in Brazil
title_fullStr	Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity Schistosoma mansoni infections in endemic area in Brazil
title_full_unstemmed	Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity Schistosoma mansoni infections in endemic area in Brazil
title_sort	Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity Schistosoma mansoni infections in endemic area in Brazil
author	Silva-Moraes, Vanessa
author_facet	Silva-Moraes, Vanessa Shollenberger, Lisa Marie Castro-Borges, William Rabello, Ana Lucia Teles Harn, Donald A. Medeiros, Lia Carolina Soares Jeremias, Wander de Jesus Siqueira, Liliane Maria Vidal Pereira, Caroline Stephane Salviano Pedrosa, Maria Luysa Camargos Almeida, Nathalie Bonatti Franco Almeida, Aureo Lambertucci, Jose Roberto Carneiro, Nídia Francisca de Figueiredo Coelho, Paulo Marcos Zech Grenfell, Rafaella Fortini Queiroz
author_role	author
author2	Shollenberger, Lisa Marie Castro-Borges, William Rabello, Ana Lucia Teles Harn, Donald A. Medeiros, Lia Carolina Soares Jeremias, Wander de Jesus Siqueira, Liliane Maria Vidal Pereira, Caroline Stephane Salviano Pedrosa, Maria Luysa Camargos Almeida, Nathalie Bonatti Franco Almeida, Aureo Lambertucci, Jose Roberto Carneiro, Nídia Francisca de Figueiredo Coelho, Paulo Marcos Zech Grenfell, Rafaella Fortini Queiroz
author2_role	author author author author author author author author author author author author author author author
dc.contributor.author.fl_str_mv	Silva-Moraes, Vanessa Shollenberger, Lisa Marie Castro-Borges, William Rabello, Ana Lucia Teles Harn, Donald A. Medeiros, Lia Carolina Soares Jeremias, Wander de Jesus Siqueira, Liliane Maria Vidal Pereira, Caroline Stephane Salviano Pedrosa, Maria Luysa Camargos Almeida, Nathalie Bonatti Franco Almeida, Aureo Lambertucci, Jose Roberto Carneiro, Nídia Francisca de Figueiredo Coelho, Paulo Marcos Zech Grenfell, Rafaella Fortini Queiroz
dc.subject.other.pt_BR.fl_str_mv	Schistosoma mansoni
topic	Schistosoma mansoni Enzyme-Linked Immunosorbent Assay Schistosomiasis mansoni Immunologic Tests Antigens Reagent Kits, Diagnostic Ensayo de Inmunoadsorción Enzimática Esquistosomiasis mansoni Pruebas Inmunológicas Juego de Reactivos para Diagnóstico Ensaio de Imunoadsorção Enzimática Esquistossomose mansoni Testes Imunológicos Antígenos Kit de Reagentes para Diagnóstico
dc.subject.en.pt_BR.fl_str_mv	Enzyme-Linked Immunosorbent Assay Schistosomiasis mansoni Immunologic Tests Antigens Reagent Kits, Diagnostic
dc.subject.es.pt_BR.fl_str_mv	Ensayo de Inmunoadsorción Enzimática Esquistosomiasis mansoni Pruebas Inmunológicas Juego de Reactivos para Diagnóstico
dc.subject.decs.pt_BR.fl_str_mv	Ensaio de Imunoadsorção Enzimática Esquistossomose mansoni Testes Imunológicos Antígenos Kit de Reagentes para Diagnóstico
description	Fundação Oswaldo Cruz. Intituto René Rachou. Biologia do Schistosoma Mansoni e sua interação com o hospedeiro. Belo Horizonte, MG, Brasil.
publishDate	2019
dc.date.accessioned.fl_str_mv	2019-07-19T14:21:06Z
dc.date.available.fl_str_mv	2019-07-19T14:21:06Z
dc.date.issued.fl_str_mv	2019
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	SILVA-MORAES, Vanessa et al. Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity Schistosoma mansoni infections in endemic area in Brazil. PLoS Neglected Tropical Diseases, v. 13, n. 3, p. 1-27, 2019.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/34288
dc.identifier.issn.pt_BR.fl_str_mv	1935-2735
dc.identifier.doi.none.fl_str_mv	10.1371/journal.pntd.0006974
dc.identifier.eissn.none.fl_str_mv	1935-2735
identifier_str_mv	SILVA-MORAES, Vanessa et al. Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity Schistosoma mansoni infections in endemic area in Brazil. PLoS Neglected Tropical Diseases, v. 13, n. 3, p. 1-27, 2019. 1935-2735 10.1371/journal.pntd.0006974
url	https://www.arca.fiocruz.br/handle/icict/34288
dc.language.iso.fl_str_mv	por
language	por
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Public Library of Science
publisher.none.fl_str_mv	Public Library of Science
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/34288/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/34288/2/journal.pntd.000697.pdf https://www.arca.fiocruz.br/bitstream/icict/34288/3/journal.pntd.000697.pdf.txt
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repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity Schistosoma mansoni infections in endemic area in Brazil

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