Functional transcriptomics of wild-caught Lutzomyia intermedia salivary glands: identification of a protective Salivary protein against Leishmania braziliensis infection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7267 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil |
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Moura, Tatiana Rodrigues deOliveira, FabianoCarneiro, Marcia WeberMiranda, José CarlosClarêncio, JorgeBarral Netto, ManoelBrodskyn, Claudia IdaBarral, Aldina Maria PradoRibeiro, José Marcos ChavesValenzuela, Jesus GOliveira, Camila Indiani de2013-11-26T19:10:35Z2013-11-26T19:10:35Z2013MOURA, T. R. de et al. Functional transcriptomics of wild-caught Lutzomyia intermedia salivary glands: identification of a protective Salivary protein against Leishmania braziliensis infection. PLoS Neglected Tropical Diseases (Online), v.7, n. 5, p. e2242-e2257, 2013.1935-2727https://www.arca.fiocruz.br/handle/icict/726710.1371/journal.pntd.0002242engPublic Library of ScienceFunctional transcriptomics of wild-caught Lutzomyia intermedia salivary glands: identification of a protective Salivary protein against Leishmania braziliensis infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilNational Institute of Allergy and Infectious Diseases. National Institutes of Health. Laboratory of Malaria and Vector Research. Rockville, Maryland, United States of AmericaFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, Bahia, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia. Salvador, Bahia, BrazilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, Bahia, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia. Salvador, Bahia, BrazilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, Bahia, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia. Salvador, Bahia, BrazilNational Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research.Rockville, Maryland, United States of AmericaNational Institute of Allergy and Infectious Diseases. National Institutes of Health. Laboratory of Malaria and Vector Research. Rockville, Maryland, United States of AmericaFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia. Salvador, BA, Brazil,Background: Leishmania parasites are transmitted in the presence of sand fly saliva. Together with the parasite, the sand fly injects salivary components that change the environment at the feeding site. Mice immunized with Phlebotomus papatasi salivary gland (SG) homogenate are protected against Leishmania major infection, while immunity to Lutzomyia intermedia SG homogenate exacerbated experimental Leishmania braziliensis infection. In humans, antibodies to Lu. intermedia saliva are associated with risk of acquiring L. braziliensis infection. Despite these important findings, there is no information regarding the repertoire of Lu. intermedia salivary proteins. Methods and Findings: A cDNA library from the Salivary Glands (SGs) of wild-caught Lu. intermedia was constructed, sequenced, and complemented by a proteomic approach based on 1D SDS PAGE and mass/mass spectrometry to validate the transcripts present in this cDNA library. We identified the most abundant transcripts and proteins reported in other sand fly species as well as novel proteins such as neurotoxin-like proteins, peptides with ML domain, and three small peptides found so far only in this sand fly species. DNA plasmids coding for ten selected transcripts were constructed and used to immunize BALB/c mice to study their immunogenicity. Plasmid Linb-11—coding for a 4.5-kDa protein—induced a cellular immune response and conferred protection against L. braziliensis infection. This protection correlated with a decreased parasite load and an increased frequency of IFN-c-producing cells. Conclusions: We identified the most abundant and novel proteins present in the SGs of Lu. intermedia, a vector of cutaneous leishmaniasis in the Americas. We also show for the first time that immunity to a single salivary protein from Lu. intermedia can protect against cutaneous leishmaniasis caused by L. braziliensis.LeishmaniaLeishmania braziliensisLutzomyiaGlândulas salivaresInfecçãoSAlivainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALMoura T R de Functional Transcriptomics....pdfMoura T R de Functional Transcriptomics....pdfapplication/pdf2866121https://www.arca.fiocruz.br/bitstream/icict/7267/1/Moura%20T%20R%20de%20Functional%20Transcriptomics....pdfaef5ca382007e522140bb8e55395da18MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7267/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTMoura T R de Functional Transcriptomics....pdf.txtMoura T R de Functional Transcriptomics....pdf.txtExtracted texttext/plain68806https://www.arca.fiocruz.br/bitstream/icict/7267/5/Moura%20T%20R%20de%20Functional%20Transcriptomics....pdf.txt13844bbfed86b66b5c4490279c91b947MD55THUMBNAILMoura T R de Functional Transcriptomics....pdf.jpgMoura T R de Functional Transcriptomics....pdf.jpgGenerated Thumbnailimage/jpeg2031https://www.arca.fiocruz.br/bitstream/icict/7267/4/Moura%20T%20R%20de%20Functional%20Transcriptomics....pdf.jpg0fd41fbb846acd09071dbad667d6a926MD54icict/72672023-03-15 14:33:57.211oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:57Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Functional transcriptomics of wild-caught Lutzomyia intermedia salivary glands: identification of a protective Salivary protein against Leishmania braziliensis infection |
| title |
Functional transcriptomics of wild-caught Lutzomyia intermedia salivary glands: identification of a protective Salivary protein against Leishmania braziliensis infection |
| spellingShingle |
Functional transcriptomics of wild-caught Lutzomyia intermedia salivary glands: identification of a protective Salivary protein against Leishmania braziliensis infection Moura, Tatiana Rodrigues de Leishmania Leishmania braziliensis Lutzomyia Glândulas salivares Infecção SAliva |
| title_short |
Functional transcriptomics of wild-caught Lutzomyia intermedia salivary glands: identification of a protective Salivary protein against Leishmania braziliensis infection |
| title_full |
Functional transcriptomics of wild-caught Lutzomyia intermedia salivary glands: identification of a protective Salivary protein against Leishmania braziliensis infection |
| title_fullStr |
Functional transcriptomics of wild-caught Lutzomyia intermedia salivary glands: identification of a protective Salivary protein against Leishmania braziliensis infection |
| title_full_unstemmed |
Functional transcriptomics of wild-caught Lutzomyia intermedia salivary glands: identification of a protective Salivary protein against Leishmania braziliensis infection |
| title_sort |
Functional transcriptomics of wild-caught Lutzomyia intermedia salivary glands: identification of a protective Salivary protein against Leishmania braziliensis infection |
| author |
Moura, Tatiana Rodrigues de |
| author_facet |
Moura, Tatiana Rodrigues de Oliveira, Fabiano Carneiro, Marcia Weber Miranda, José Carlos Clarêncio, Jorge Barral Netto, Manoel Brodskyn, Claudia Ida Barral, Aldina Maria Prado Ribeiro, José Marcos Chaves Valenzuela, Jesus G Oliveira, Camila Indiani de |
| author_role |
author |
| author2 |
Oliveira, Fabiano Carneiro, Marcia Weber Miranda, José Carlos Clarêncio, Jorge Barral Netto, Manoel Brodskyn, Claudia Ida Barral, Aldina Maria Prado Ribeiro, José Marcos Chaves Valenzuela, Jesus G Oliveira, Camila Indiani de |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Moura, Tatiana Rodrigues de Oliveira, Fabiano Carneiro, Marcia Weber Miranda, José Carlos Clarêncio, Jorge Barral Netto, Manoel Brodskyn, Claudia Ida Barral, Aldina Maria Prado Ribeiro, José Marcos Chaves Valenzuela, Jesus G Oliveira, Camila Indiani de |
| dc.subject.decs.pt_BR.fl_str_mv |
Leishmania Leishmania braziliensis Lutzomyia Glândulas salivares Infecção SAliva |
| topic |
Leishmania Leishmania braziliensis Lutzomyia Glândulas salivares Infecção SAliva |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil |
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2013 |
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2013-11-26T19:10:35Z |
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2013-11-26T19:10:35Z |
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2013 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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MOURA, T. R. de et al. Functional transcriptomics of wild-caught Lutzomyia intermedia salivary glands: identification of a protective Salivary protein against Leishmania braziliensis infection. PLoS Neglected Tropical Diseases (Online), v.7, n. 5, p. e2242-e2257, 2013. |
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https://www.arca.fiocruz.br/handle/icict/7267 |
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1935-2727 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pntd.0002242 |
| identifier_str_mv |
MOURA, T. R. de et al. Functional transcriptomics of wild-caught Lutzomyia intermedia salivary glands: identification of a protective Salivary protein against Leishmania braziliensis infection. PLoS Neglected Tropical Diseases (Online), v.7, n. 5, p. e2242-e2257, 2013. 1935-2727 10.1371/journal.pntd.0002242 |
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Public Library of Science |
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Public Library of Science |
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